FastQCFastQC Report
Thu 24 Aug 2023
SRR10970369_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10970369_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9909859
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length125
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG147731314.907507765751257TruSeq Adapter, Index 8 (100% over 49bp)
GAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAAC8345318.421219716647835Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAACT2069372.0881931821633386Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAA2022012.040402391194466Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTGA1064591.0742736097456078Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAGGGCGCACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTG827030.8345527418704948TruSeq Adapter, Index 8 (100% over 38bp)
AACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTG679890.6860743427328281Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC661540.6675574294245761TruSeq Adapter, Index 5 (100% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC583570.5888782070461346TruSeq Adapter, Index 8 (100% over 50bp)
AGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAAC565920.5710676610030475Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGGCAATTTTTGACAGTCTCAGATCGGAAGAGCACACGTCTGAACTCCAG426580.43046021139150414Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAA424100.42795765308063416Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGT387590.3911155547218179TruSeq Adapter, Index 8 (100% over 46bp)
TTGGTGACTCTAGATAACCTAGATCGGAAGAGCACACGTCTGAACTCCAG365070.3683907107053693Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCG354610.35783556557161916TruSeq Adapter, Index 8 (100% over 45bp)
GAGGGCGCACCACCGGCCCGTCTCAGATCGGAAGAGCACACGTCTGAACT326800.3297726032227098Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCG313030.3158773500208227TruSeq Adapter, Index 8 (100% over 45bp)
GGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCG291940.2945955134174966TruSeq Adapter, Index 8 (100% over 45bp)
GAGGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAAT284180.28676492773509693TruSeq Adapter, Index 8 (100% over 41bp)
CGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCG278360.28089198847329716TruSeq Adapter, Index 8 (100% over 45bp)
GAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCG274810.2773096973428179TruSeq Adapter, Index 8 (100% over 45bp)
GGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCG259380.2617393446263968TruSeq Adapter, Index 8 (100% over 45bp)
GGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGT252600.254897673115228TruSeq Adapter, Index 8 (100% over 46bp)
GGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCG248380.2506392876023766TruSeq Adapter, Index 8 (100% over 45bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTAT242390.24459480200475103TruSeq Adapter, Index 8 (100% over 48bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC240820.2430105211385954TruSeq Adapter, Index 8 (100% over 50bp)
ACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA234880.2370164903456245Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGT228260.2303362742093505TruSeq Adapter, Index 8 (100% over 46bp)
GCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCG225220.22726862208634854TruSeq Adapter, Index 8 (100% over 45bp)
TGGCAATTTTTGACAGTCTAGATCGGAAGAGCACACGTCTGAACTCCAGT214060.2160071096874335Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTC211840.2137669163607676TruSeq Adapter, Index 8 (100% over 44bp)
GAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCG204600.2064610606467761TruSeq Adapter, Index 8 (100% over 45bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC200160.2019806739934443TruSeq Adapter, Index 8 (98% over 50bp)
CACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC199330.2011431242361773Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTCTAGCATCACCAGTATTAGAGGAGATCGGAAGAGCACACGTCTGAACT187890.1895990649311963Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCG185320.18700568797194791TruSeq Adapter, Index 8 (100% over 45bp)
AAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCT176900.178509098868107Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCG175370.1769651818456751TruSeq Adapter, Index 8 (100% over 50bp)
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTAT172140.17370580146498552TruSeq Adapter, Index 8 (100% over 48bp)
GGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTC168670.1702042380219537TruSeq Adapter, Index 8 (100% over 44bp)
AGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGT166730.16824659160135375TruSeq Adapter, Index 8 (100% over 46bp)
ACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA163060.1645432089397034Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGT162180.16365520437778175TruSeq Adapter, Index 8 (100% over 46bp)
GGAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCG159510.1609609178092241TruSeq Adapter, Index 8 (100% over 45bp)
GGGAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATC157930.1593665459821376TruSeq Adapter, Index 8 (100% over 42bp)
CGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTG155890.15730798995222836Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCG152460.15384679035292026TruSeq Adapter, Index 8 (100% over 45bp)
GCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGT149050.150405772675474TruSeq Adapter, Index 8 (100% over 46bp)
GGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCG146890.14822612511439365TruSeq Adapter, Index 8 (100% over 45bp)
GAGGGCGCACCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACT145770.14709593749012978TruSeq Adapter, Index 8 (100% over 36bp)
CGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGA144790.14610702331889888Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGGTGACTCTAGATAACCTCAGATCGGAAGAGCACACGTCTGAACTCCA143730.14503738146022058Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTGAA142340.14363473789082165Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCG135010.13623806352845183TruSeq Adapter, Index 8 (100% over 45bp)
GGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGT134520.13574360644283637TruSeq Adapter, Index 8 (100% over 46bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTAT133900.13511796686511887TruSeq Adapter, Index 8 (100% over 48bp)
GGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTC133330.134542782092056TruSeq Adapter, Index 8 (100% over 44bp)
GGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAACTC125070.1262076483631099Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCG122990.124108728489477TruSeq Adapter, Index 8 (100% over 45bp)
ACGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTC119390.12047598255434311TruSeq Adapter, Index 8 (100% over 44bp)
GCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTA116190.1172468750564463TruSeq Adapter, Index 8 (100% over 47bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTAT115130.11617723319776802TruSeq Adapter, Index 8 (100% over 48bp)
GACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGT114900.11594514109635667TruSeq Adapter, Index 8 (100% over 46bp)
GGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCG113270.1143003144646155TruSeq Adapter, Index 8 (100% over 45bp)
GAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGT109380.1103749306624847TruSeq Adapter, Index 8 (100% over 46bp)
CGCTTGACTAGCTTGCTGTTTAGATCGGAAGAGCACACGTCTGAACTCCA109270.1102639300922445Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACGGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATC109210.11020338432665895TruSeq Adapter, Index 8 (100% over 42bp)
CCGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTC108720.10970892724104347TruSeq Adapter, Index 8 (100% over 44bp)
GGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTC106920.10789255427347655TruSeq Adapter, Index 8 (100% over 50bp)
CGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCG105100.10605599938404774TruSeq Adapter, Index 8 (100% over 45bp)
GGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGT104900.10585418016542919TruSeq Adapter, Index 8 (100% over 46bp)
ACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCG102970.1039066247057602TruSeq Adapter, Index 8 (100% over 45bp)
CGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT102630.10356353203410866TruSeq Adapter, Index 8 (100% over 43bp)
AGATCGGAAGAGCACACGTCTGAACTCCCGTCACACTTGAATCTCGTATG102600.10353325915131587TruSeq Adapter, Index 8 (97% over 49bp)
AGGGCGCACCACCGGCCCGTCTCAGATCGGAAGAGCACACGTCTGAACTC102130.10305898398756227Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCG101760.10268561843311796TruSeq Adapter, Index 8 (100% over 45bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT101370.10229207095681178Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTC101300.10222143423029531TruSeq Adapter, Index 8 (100% over 44bp)
TAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTC100080.10099033699672215No Hit
TTAGCAGTAAACTGAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTC99960.10086924546555104Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCG99840.1007481539343799TruSeq Adapter, Index 8 (100% over 45bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)