FastQCFastQC Report
Thu 24 Aug 2023
SRR10970371_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10970371_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10805514
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length125
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG159378314.749719448792534TruSeq Adapter, Index 12 (100% over 49bp)
GAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAAC127967411.842786932671597Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAA2937272.7183065979091787Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAACT2199812.035821711026426Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTGA1417261.311608128960825Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGCAATTTTTGACAGTCTCAGATCGGAAGAGCACACGTCTGAACTCCAG1070220.9904387704277648Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTGGTGACTCTAGATAACCTAGATCGGAAGAGCACACGTCTGAACTCCAG929330.8600516365996103Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAA876480.8111414227958059Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTG824520.7630548625451783Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAAC611510.5659240273067991Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC482310.446355444081605TruSeq Adapter, Index 12 (100% over 50bp)
TGGCAATTTTTGACAGTCTAGATCGGAAGAGCACACGTCTGAACTCCAGT432600.4003511540496824Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTCTAGCATCACCAGTATTAGAGGAGATCGGAAGAGCACACGTCTGAACT361710.3347457603590167Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGT338860.3135991494712792TruSeq Adapter, Index 12 (100% over 46bp)
CCTCTAGCATCACCAGTATTAGAGGCAGATCGGAAGAGCACACGTCTGAA333540.30867573722083Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CAAGCATCAAGCACGCAGCAGATCGGAAGAGCACACGTCTGAACTCCAGT318860.29509008086056804Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTGGTGACTCTAGATAACCTCAGATCGGAAGAGCACACGTCTGAACTCCA318130.2944144998562771Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCG292170.270389728799574TruSeq Adapter, Index 12 (100% over 45bp)
CGCTTGACTAGCTTGCTGTTTAGATCGGAAGAGCACACGTCTGAACTCCA291010.26931620282015273Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCG286340.2649943352995517TruSeq Adapter, Index 12 (100% over 45bp)
ATTGATCATCGACACTTCGAACGAGATCGGAAGAGCACACGTCTGAACTC282080.2610519036854702Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGA280930.2599876322403543Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC265650.245846703821771TruSeq Adapter, Index 12 (100% over 50bp)
TTAGCAGTAAACTGAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTC261090.24162663617852886Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGT256090.23699936902585103TruSeq Adapter, Index 12 (100% over 46bp)
GGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCG252780.23393611817077836TruSeq Adapter, Index 12 (100% over 45bp)
GGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCG251350.2326127197651125TruSeq Adapter, Index 12 (100% over 45bp)
GAGGGCGCACCACCGGCCCGTCTCAGATCGGAAGAGCACACGTCTGAACT241220.22323787651378735Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCG240630.2226918589897713TruSeq Adapter, Index 12 (100% over 45bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTAT235310.2177684467393222TruSeq Adapter, Index 12 (100% over 48bp)
CGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTGAA231680.2144090507864781Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCG229640.21252112578818552TruSeq Adapter, Index 12 (100% over 45bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC229630.21251187125388019No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT229080.21200287186708563Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCG228930.21186405385250529TruSeq Adapter, Index 12 (100% over 45bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCG221320.20482135324612968TruSeq Adapter, Index 12 (100% over 50bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC217440.20123059393565174TruSeq Adapter, Index 12 (98% over 50bp)
TTAGCAGTAAACTGAGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGT212160.19634419982242401Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA196400.1817590537571836Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGTCTGAACTCCAG192420.1780757491036521Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTTGGTGACTCTAGATAACCTAGATCGGAAGAGCACACGTCTGAACTCCA192130.17780736760879678Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTGATCATCGACACTTCGAACGAGATCGGAAGAGCACACGTCTGAACTCC189950.17578987913022925Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCTTCTGGCGCCAAGCGCCCGGAGATCGGAAGAGCACACGTCTGAACTC184550.17079243060533722Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCT184540.17078317607103188Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGT183160.1695060503368928TruSeq Adapter, Index 12 (100% over 46bp)
GCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCG181510.16797905217650913TruSeq Adapter, Index 12 (100% over 45bp)
GGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTC181420.16789576136776094TruSeq Adapter, Index 12 (100% over 44bp)
CGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTG172330.15948338968419273Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC170050.15737335586257165Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCG168870.1562813208145397TruSeq Adapter, Index 12 (100% over 45bp)
ACCATGATCACGAAGGTGGTAGATCGGAAGAGCACACGTCTGAACTCCAG161920.14984941947231756Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACGATTAAAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGA159640.14773938565069647Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTAT157700.14594400599545748TruSeq Adapter, Index 12 (100% over 48bp)
CGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGT156790.14510184337367013TruSeq Adapter, Index 12 (100% over 46bp)
GACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCG154310.14280671886594196TruSeq Adapter, Index 12 (100% over 45bp)
GCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCG152850.14145555685736003TruSeq Adapter, Index 12 (100% over 45bp)
GGAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCG148860.13776299766952316TruSeq Adapter, Index 12 (100% over 45bp)
GCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGT144660.13387609326127384TruSeq Adapter, Index 12 (100% over 46bp)
GGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTC143980.13324678492850964TruSeq Adapter, Index 12 (100% over 44bp)
AACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGTCTGAACTCCA142360.13174755037104205Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCG139900.12947093493192458TruSeq Adapter, Index 12 (100% over 45bp)
GACCTGGTATTGCAGTACCTCCAGGAGATCGGAAGAGCACACGTCTGAAC138180.12787915503140343Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACAAGCATCAAGCACGCAGCAGATCGGAAGAGCACACGTCTGAACTCCAG138140.127842136894182Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATC137500.12724984669863923TruSeq Adapter, Index 12 (100% over 42bp)
GGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGT137470.12722208309572317TruSeq Adapter, Index 12 (100% over 46bp)
TTGGTGACTCTAGATAACCTCGAGATCGGAAGAGCACACGTCTGAACTCC133290.12335368775608453Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGT130100.1204014913126761TruSeq Adapter, Index 12 (100% over 46bp)
GGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAACTC127220.1177361854327337Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTAT125620.1162554599438768TruSeq Adapter, Index 12 (100% over 48bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTC123940.11470069818057707TruSeq Adapter, Index 12 (100% over 50bp)
GGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCG123400.11420095332808787TruSeq Adapter, Index 12 (100% over 45bp)
GGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCG120630.11163744732550437TruSeq Adapter, Index 12 (100% over 45bp)
CGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCG118840.10998088568484572TruSeq Adapter, Index 12 (100% over 45bp)
GGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTC115590.10697316203560514TruSeq Adapter, Index 12 (100% over 44bp)
TTTGGTGACTCTAGATAACCTCAGATCGGAAGAGCACACGTCTGAACTCC113060.10463176485635019Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTCGGTTTCTATCTACTTCAAATTCCAGATCGGAAGAGCACACGTCTGAA112330.10395618385205924Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTAT110120.10191093177057564TruSeq Adapter, Index 12 (100% over 48bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGT109900.10170733201585783TruSeq Adapter, Index 12 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCCGTCACCTTGTAATCTCGTATG108890.1007726240510169TruSeq Adapter, Index 12 (97% over 49bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)