FastQCFastQC Report
Thu 24 Aug 2023
SRR10970373_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10970373_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10150606
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length125
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG258230425.439899844403378TruSeq Adapter, Index 7 (100% over 49bp)
GAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAAC112568811.089860053675613Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAA2680462.640689629762006Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAACT1585021.5615028304714025Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC875840.8628450360500645TruSeq Adapter, Index 7 (100% over 50bp)
ACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTGA731650.7207944038020981Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAA554880.5466471656963141Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAAC496740.4893697972318106Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTG425260.4189503562644437Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCG414720.4085667397591829TruSeq Adapter, Index 7 (100% over 45bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTAT392000.3861838396643511TruSeq Adapter, Index 7 (100% over 48bp)
GGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGT371170.365662897367901TruSeq Adapter, Index 7 (100% over 46bp)
GAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCG366820.36137743894305424TruSeq Adapter, Index 7 (100% over 45bp)
CGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTG354240.3489840902109687Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTAT348400.34323073913025487TruSeq Adapter, Index 7 (100% over 48bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT312340.3077057665325597Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC302880.2983861259120884TruSeq Adapter, Index 7 (100% over 50bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC266510.2625557528289444TruSeq Adapter, Index 7 (98% over 50bp)
GGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTC248460.2447735632729711TruSeq Adapter, Index 7 (100% over 44bp)
GGAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCG242060.23846852099273677TruSeq Adapter, Index 7 (100% over 45bp)
GGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCG241560.23797593956459348TruSeq Adapter, Index 7 (100% over 45bp)
GGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGT232030.2285873375441821TruSeq Adapter, Index 7 (100% over 46bp)
GCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCG223450.220134640237243TruSeq Adapter, Index 7 (100% over 45bp)
GGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTC213580.21041108284569413TruSeq Adapter, Index 7 (100% over 44bp)
GGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCG213070.20990864978898796TruSeq Adapter, Index 7 (100% over 45bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCG210870.20774129150515744TruSeq Adapter, Index 7 (100% over 50bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTAT209390.20628325047785326TruSeq Adapter, Index 7 (100% over 48bp)
GGGAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATC206230.20317013585198757TruSeq Adapter, Index 7 (100% over 42bp)
CGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCG202720.19971221422642157TruSeq Adapter, Index 7 (100% over 45bp)
GGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCG201280.19829357971336883TruSeq Adapter, Index 7 (100% over 45bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTAT199360.19640206702929855TruSeq Adapter, Index 7 (100% over 48bp)
GAGGGCGCACCACCGGCCCGTCTCAGATCGGAAGAGCACACGTCTGAACT198140.1952001683446289Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGA190300.18747649155134186Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTC183090.1803734673575154TruSeq Adapter, Index 7 (100% over 50bp)
GGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCG181890.17919127192997147TruSeq Adapter, Index 7 (100% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCCGTCACCAGATCATCTCGTATG176780.17415708973434688TruSeq Adapter, Index 7 (97% over 49bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC174640.17204884122189354No Hit
CGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGT172820.17025584482345194TruSeq Adapter, Index 7 (100% over 46bp)
GAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCG168170.1656748375417192TruSeq Adapter, Index 7 (100% over 45bp)
CTCTAGCATCACCAGTATTAGAGGAGATCGGAAGAGCACACGTCTGAACT156190.1538725865234056Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGT154750.15245395201035286TruSeq Adapter, Index 7 (100% over 46bp)
GCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCG151500.14925217272742142TruSeq Adapter, Index 7 (100% over 45bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG149500.1472818470148482TruSeq Adapter, Index 7 (97% over 49bp)
AGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGT149410.1471931823577824TruSeq Adapter, Index 7 (100% over 46bp)
GGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCG148680.14647401347269315TruSeq Adapter, Index 7 (100% over 45bp)
GACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTC148030.14583365761610687TruSeq Adapter, Index 7 (100% over 44bp)
GACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCG145140.14298653696143857TruSeq Adapter, Index 7 (100% over 45bp)
GCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTC142450.1403364488780276Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTA137710.13566677693922904TruSeq Adapter, Index 7 (100% over 47bp)
ACATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG135850.13383437402653595TruSeq Adapter, Index 7 (97% over 49bp)
CGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGT133370.13139117014294516TruSeq Adapter, Index 7 (100% over 46bp)
TGGCAATTTTTGACAGTCTCAGATCGGAAGAGCACACGTCTGAACTCCAG130060.12813028108863647Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCG127000.12511568274839946TruSeq Adapter, Index 7 (100% over 45bp)
ATATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG118440.11668268869858608TruSeq Adapter, Index 7 (97% over 49bp)
GGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGT117640.11589455841355678TruSeq Adapter, Index 7 (100% over 46bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGT117550.11580589375649099TruSeq Adapter, Index 7 (100% over 50bp)
GCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGT115560.11384541967248064TruSeq Adapter, Index 7 (100% over 46bp)
GGGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCT113660.11197361024553609TruSeq Adapter, Index 7 (100% over 43bp)
AAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCT112320.11065349201811203Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTGAA111080.10943189007631662Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTA110800.10915604447655637TruSeq Adapter, Index 7 (100% over 47bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA110760.1091166379623049Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTC108420.10681135687859425TruSeq Adapter, Index 7 (100% over 44bp)
GACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCG107270.10567841959386465TruSeq Adapter, Index 7 (100% over 45bp)
GCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCG105140.10358002270997417TruSeq Adapter, Index 7 (100% over 45bp)
GGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCG102580.10105800579788043TruSeq Adapter, Index 7 (100% over 45bp)
TTGGTGACTCTAGATAACCTAGATCGGAAGAGCACACGTCTGAACTCCAG102110.10059497925542574Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)