FastQCFastQC Report
Thu 24 Aug 2023
SRR11005876_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11005876_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32335621
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC13444904.157922311125554No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT10950013.3863614371284227No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC6555392.027296769714118No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC5424201.6774689436148449No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACG5294621.6373954902551584No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC5215501.6129271183627492No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT5114451.5816767520871178No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT4684791.448801617262894No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACACG4447001.3752635213036424No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCT4370881.3517229188207023Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT2906240.8987735228588929No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT2893750.8949109095508014No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG2675320.8273600188473264No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGATCACACGTC1650560.5104463588313334No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCTGAAGAGCACAC1629280.5038653811534963No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT1342830.41527886537264896Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC1334620.41273986975540067No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAATAGCACACGT1274420.3941226302720458No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC1256580.3886054948504004No Hit
ATTGATCATCGACACTTCGAACGCAAGATCGGAAGAGCACACGTCTGAAC1182460.365683405307107Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAATAGCACACGTC1169970.36182079199901557No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA1123950.34758880925775326Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCT1072740.3317517854381087Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGTAAGAGCACAC1030960.31883105012889656No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCT977050.3021590338407294Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACAC931010.2879208659700706No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT928880.2872621496893472Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGATCACACGT923070.2854653695996746No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC921120.2848623194835194No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAATAGCACACGT896400.2772174995494906No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAATAGCACAC870340.2691582759458988No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGATCACACGTC842190.26045270632037654No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG811800.25105440220245034No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACGT798470.2469320134597075No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACG787370.24349926664467028No Hit
ATTGATCATCGACACTTCGAACGCACAGATCGGAAGAGCACACGTCTGAA762740.23588227979292556Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGCCACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGA739560.22871371482242447Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC723390.22371303770538378Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACAC715100.22114930157054968No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGT710920.21985660952668884No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTG630770.19506970347036168Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAATAGCACACGTC627990.19420997048425329No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTG625430.19341827392150593Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACG625020.19329147876887845No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAATAGCACACG608460.18817019162860674No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAAGATCGGAAGAGCACAC602280.18625898664509954No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGATCACACGTC601660.18606724763380916No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAATAGCACACGT585180.1809707010111233No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTGA581990.17998417287238738Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACG573490.17735549287889044No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGATCACACGT556610.17213524366827532No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGT537470.16621607483585982No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGATCACACGTCT534930.16543056340250897Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAATAGCACACGT523810.16199163145807527No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAATAGCACACG517580.16006496365107695No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGT509570.15758781932779334No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA486000.15029864433406118Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCGCCTGGGAATACCAGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT484590.1498625927116105No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGTAAGAGCACACG475630.1470916547419949No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT473770.1465164377081238No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCTTAAGAGCACAC453600.14027873471179045No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACGTC444130.13735007594256501No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTC437460.13528733528884446No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTC433160.1339575324686048No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCT432420.13372868268093566Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACG430700.13319676155283983No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGTAAGAGCACACG429210.1327359694128033No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGATCACACGT423610.13100413318179355No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAATAGCACACGTC416720.1288733561047119No Hit
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA412000.1274136655671465Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAATATCACACGTC406290.1256478111244562No Hit
TTGATCATCGACACTTCGAACGCAAGATCGGAAGAGCACACGTCTGAACT398870.12335312811836828Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGT391750.12115122205322731No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGTGCACACGTC390730.12083578045400767No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTC384720.11897714907037041No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAATAGCACACGT383840.11870500337692602No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGTGCACACGT382820.11838956177770638No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA374640.11585984385455285Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGATCATCGACACTTCGAACGCACAGATCGGAAGAGCACACGTCTGAAC371890.11500938856253912Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCAGATCGGAAGAGCACACGTCT370580.11460426258707078Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTC367820.11375071473035882No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGATCACACGT363360.11237143087494748No Hit
CGCCACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGAA357760.11063959464393772Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAATAGCACACGTCT354000.1094767903174026Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTAGATCGGAAGAGCACACGTC349190.10798926669755315No Hit
AGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGAGATCGGAAGAGC347170.10736456862851035No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAAGATCGGAAGAGCACACGT346350.10711097832325532No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACGT344520.10654503898347893No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGACAGATCGGAAGAGCACA339900.10511627409289588No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACAC339580.10501731202255246No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA330720.10227729969991917No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCTGAATAGCACAC326090.10084544224463789No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)