FastQCFastQC Report
Thu 24 Aug 2023
SRR11005878_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11005878_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34117395
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC14575284.272096389539706No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT11880553.48225589908022No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC7086112.0769786204368765No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC6002691.759422136420439No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACG5759151.688039195255089No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC5676871.6639224653582136No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT5607161.6434900730257982No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT5055631.4818335338908495No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACACG4812481.4105649039148505No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCT4734891.387822839346322Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT3140450.9204835246067291No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT3126160.9162950453866715No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG2937650.861041706144329No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGATCACACGTC1849820.5421926263713862No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCTGAAGAGCACAC1783110.5226395508801303No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT1450120.4250383125675334Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC1447260.4242000305122944No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAATAGCACACGT1416730.4152515161254252No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC1357280.3978263873897758No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAATAGCACACGTC1270460.3723789580066122No Hit
ATTGATCATCGACACTTCGAACGCAAGATCGGAAGAGCACACGTCTGAAC1252640.36715581597012314Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA1199260.3515098383097537Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCT1156030.3388388826286415Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGTAAGAGCACAC1099830.3223663471375819No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCT1058000.31010573931567753Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACAC1029370.30171412559487615No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGATCACACGT1028340.30141222681274465No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAATAGCACACGT1016250.29786857994287075No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT1008130.295488562359465Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC1001640.29358630692642274No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGATCACACGTC950950.2787287833669599No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAATAGCACAC914180.26795128995047834No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACGT872600.255763958532004No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG859430.2519037575993126No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACG840760.24643147579116167No Hit
ATTGATCATCGACACTTCGAACGCACAGATCGGAAGAGCACACGTCTGAA819490.24019711938733893Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGCCACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGA796540.2334703455524667Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACAC778460.2281709960564105No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGT769310.22548907969087323No Hit
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC768690.22530735421036688Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAATAGCACACG696690.20420375002253252No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTG692020.20283494680646044Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTG685190.2008330354647534Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGATCACACGTC681180.1996576819537365No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAATAGCACACGTC674070.19757370104018784No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACG674010.19755611470336468No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAATAGCACACGT671970.19695817925137601No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAAGATCGGAAGAGCACAC658020.1928693559399831No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGATCACACGT636690.18661741319933717No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTGA633680.1857351653020402Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACG619160.18147927179082696No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAATAGCACACGT596720.1749019818189519No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAATAGCACACG588060.17236368720413736No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGT585600.17164264739438637No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGATCACACGTCT580980.17028849945900032Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGT557220.16332431007701498No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA522900.15326492541414724Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT517760.15175836255962685No Hit
ACCGCCTGGGAATACCAGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT516520.15139491159861412No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGTAAGAGCACACG500340.1466524627686258No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGATCACACGT489370.14343709418611825No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACGTC489050.14334330038972787No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTC478820.14034482996137307No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTC475220.13928964975198135No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCTTAAGAGCACAC472250.13841912607923318No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCT470720.13797067449024172Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACG469400.13758377508013142No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGTAAGAGCACACG454850.13331908840050655No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAATAGCACACGTC449880.13186235350031852No Hit
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA438200.12843887993206984Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAATAGCACACGT428000.12544920267212664No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGT425730.12478385292898242No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTC420540.12326263479377601No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAATATCACACGTC418710.12272625152066857No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGATCACACGT417730.12243900801922304No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCTGAATAGCACAC415700.1218440036233716No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA409040.11989192023599692Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCAGATCGGAAGAGCACACGTCT400310.117333108228222Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGATCATCGACACTTCGAACGCAAGATCGGAAGAGCACACGTCTGAACT400220.11730672872298721Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTGATCATCGACACTTCGAACGCACAGATCGGAAGAGCACACGTCTGAAC393310.11528136893218255Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCCGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTC393010.11519343724806658No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACGT383530.1124147960300017No Hit
CGCCACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGAA381550.11183444691483625Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAATAGCACACGTCT379520.11123944251898482Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAAGATCGGAAGAGCACACGT376950.11048616109172463No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACAC376010.11021064181482788No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTAGATCGGAAGAGCACACGTC372140.1090763230897318No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGACAGATCGGAAGAGCACA368310.10795372858918449No Hit
AGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGAGATCGGAAGAGC366540.10743493165290023No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA355200.10411111399331631No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGTGCACACGT347170.10175747591514533No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAATAGCACACG346110.10144678396460222No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGTGCACACGTC345240.1011917820806659No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)