FastQCFastQC Report
Thu 24 Aug 2023
SRR11005880_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11005880_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37123934
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC21629095.826184800350092No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT18057744.864177379477078No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC12923303.48112352532466No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT8295502.2345422766886722No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC5694461.5339053237191942No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCTGAAGAGCACAC5685061.5313732644821532No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT3739121.007199290894117No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACACG3703440.9975882405135188No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGTAAGAGCACAC3616130.9740697200894712No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA2974850.8013294065224876No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAATAGCACAC2782210.7494383542433838No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT2458390.6622116072073612No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG2388230.6433127480508936No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC2374360.6395766138362384No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT2250520.6062180802282431No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG2062640.5556092196478962No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAATAGCACACGT1934810.5211759077041781No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA1696110.4568777651635735Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGCCACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGA1601160.43130127318942Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCTTAAGAGCACAC1534280.4132859410858774No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGATCACACGTC1430290.3852743623561016No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACAC1386380.37344641330307293No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA1356670.36544348990599973Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACG1350920.36389462388334165No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGATCACACGT1279610.3446859915223424No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA1127480.3037070370828695Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC1088220.29313164924816426No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATTGGAAGAGCACAC1063850.28656715099213353No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCTGAATAGCACAC1053170.2836903007100487No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAATAGCACACGTC1050370.2829360702990152No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAATAGCACACGT1024560.27598368211731006No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCT920390.24792361714682504Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGATGAGCACAC858850.23134671018432473No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGTAGAGCACAC855350.2304039221705329No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGT854290.23011839208635596No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTG781380.21047877091905182Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCTGAAGAGCACA770230.20747531767511493No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGTAAGAGCACA769920.2073918135938934No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACG765700.20625508061726433No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACG749890.20199637247496455No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGTAATAGCACAC747880.20145494278704404No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACG734410.19782655577396513No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGT726180.19560965710153458No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTGA710600.1914129036001411Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC674950.18180993425966116No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGATCACACGTC656310.17678891466620966No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAATAGCACACGT655250.1765033845820327No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGATCACACGT651440.1754770924870193No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACA613310.16520609049676685No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACAC605520.16310771374607014No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT599090.1613756774807325Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCTTAATAGCACAC580590.1563923694078327No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG574070.15463609002214043Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA572650.15425358745654488Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT571400.15391687745161922No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAAGATCGGAAGAGCACAC567840.15295792735759092No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGTGCACACGT556670.1499490867535752No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC533390.14367819962183964No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACG530940.14301824801218535No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACAC518260.1396026617222194No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAATAGCACACGTC498520.13428533732443335No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT458060.12338670788499947No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACA449560.12109707985150497No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAATATCACACGT439720.11844649869273015No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTC433130.11667136354676204No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGTAAGAGCACACGT432820.11658785946554047No Hit
AGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGAGATCGGAAGAGC427550.11516829008477389No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAATAGCACACG396380.10677208940194755No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAATAGCACACG389680.10496732377554599No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACG381470.10275581246319423No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGATCACACGT378530.10196387053160907No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAATAGCACACGT376780.10149247652471315No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAATATCACACGTC372050.10021836586607442No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)