FastQCFastQC Report
Thu 24 Aug 2023
SRR11005887_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11005887_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40184673
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC16739074.165535949489996No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT16254814.045027316758308No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC14762453.6736518921032406No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT6902251.7176324913730168No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC6508681.6196921647216094No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT6107331.5198157765275333No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACACG4714951.17332048465344No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCTGAAGAGCACAC4658611.1593002137904669No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGTAAGAGCACAC3162890.7870886494460213No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT2538070.6316015063753286No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGA2491560.620027441805984Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAATAGCACAC2320350.5774216453123806No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG1960900.48797211812573416No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAATAGCACACGT1935730.48170853598833563No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCT1893230.4711323643220887Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC1878630.4674991382908603No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA1799000.4476831253547839Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGATCACACGTC1782480.4435721052153392No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG1745500.4343695916102142No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA1728090.43003709399352336No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC1415020.35212928073347766No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT1406150.34992197149395743No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGATCACACGT1342500.33408259910438987No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAATAGCACACGTC1262830.3142566321243923No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA1260390.3136494354452007Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACG1238330.30815978022267343No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAATAGCACACGT1190080.2961527147427578No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA1099200.2735371269538513Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCTGAATAGCACAC1090060.27126262791786315No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACAC1082800.2694559689461701No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACG1050350.261380750815118No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCTTAAGAGCACAC1044680.2599697650892916No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC1042990.25954920673362203No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTG991470.24672839816315043Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT962260.23945945758971338Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACAC954320.2374835798713604No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAATAGCACACGT951090.23667979082472565No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTGA855790.21296428118252947Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGATCACACGTC819540.20394342887896585No Hit
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA777480.19347675169585182Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC763520.1900027903673622No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGT754420.1877382453752952No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTC747460.186006241733011No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGATCACACGT732050.18217144631237886No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGTAATAGCACAC686530.17084374433008326No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACG681750.169654236081503No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGT673410.16757881792393833No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACG657160.16353498758096152No Hit
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC650360.161842800114362Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGATCACACGT636500.15839372389567535No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAATAGCACACGTC603030.15006467764463333No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG598680.14898217536820568No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAAGATCGGAAGAGCACAC595460.1481808748325512No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATTGGAAGAGCACAC587620.14622988222400116No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT584440.14543853573226787No Hit
ACCGCCTGGGAATACCAGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT576060.143353163530782No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGTAGAGCACAC567590.14124539473047348No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAATAGCACACG555110.1381397330270673No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGATGAGCACAC534570.13302833147354465No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG502220.12497799845229549Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACAC494000.1229324424264943No Hit
CGCCACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGAA493740.12286774114100667Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTC492220.12248948747200208No Hit
ATTGATCATCGACACTTCGAACGCACAGATCGGAAGAGCACACGTCTGAA490120.12196690016614045Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGTGCACACGT484670.12061066168188055No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCTGAAGAGCACA473980.11795044344394691No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGTAAGAGCACA466610.11611641085147066No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGTAAGAGCACACG451060.11224677627711441No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCTTAATAGCACAC440750.10968112145643191No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAATATCACACGTC432800.10770275522709864No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT427090.10628181545735112No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT410780.10222305404849258Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAATAGCACACG404100.10056072871365658No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)