FastQCFastQC Report
Thu 24 Aug 2023
SRR11005888_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11005888_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40320582
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC17295804.289571018592936No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT16520414.09726476666433No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC15035093.7288871475119083No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT6999691.7360091677248113No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC6588431.6340116320741602No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT6222091.5431548086285063No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACACG4783771.186433767250681No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCTGAAGAGCACAC4696861.1648790188594005No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGTAAGAGCACAC2939600.7290569367277486No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT2582250.6404297437968529No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGA2538830.6296610500314703Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAATAGCACAC2466420.6117024798897992No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG1979900.491039539062209No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAATAGCACACGT1970560.4887231042448742No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCT1921270.47649857831913234Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC1911940.4741846236247284No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGATCACACGTC1901740.4716548982353479No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA1815180.450186954146644Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG1777250.44077984787025143No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA1736960.43078743258219837No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC1445980.3586208155427915No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT1438270.35670864076317155No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGATCACACGT1400610.3473684978059096No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAATAGCACACGTC1306570.3240454217649934No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA1283410.3183014570573411Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACG1261620.31289726919120364No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAATAGCACACGT1225630.3039713067633796No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCTTAAGAGCACAC1167110.28945762737254144No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA1119340.2776100801322759Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACAC1100720.272992091235191No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACG1073090.26613951157748666No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC1054800.26160336673711704No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCTGAATAGCACAC1050460.26052699338516494No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTG1011600.2508892356762112Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT987850.2449989437156438Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACAC981270.24336702282720027No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAATAGCACACGT959030.23785122942917838No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTGA878020.2177597535670492Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGATCACACGTC863610.2141858964238165No Hit
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA788830.19563953714755405Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC778450.19306516954541975No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGTAATAGCACAC775640.19236825500187474No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGT767840.19043375911587784No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGATCACACGT767280.190294872231755No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTC758130.1880255597501048No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACG695220.1724231063926607No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGT686470.1702529988282411No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACG671150.16645345049830879No Hit
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC665460.16504226055070334Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGATCACACGT659540.16357402777569033No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAATAGCACACGTC617040.1530335053199381No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAAGATCGGAAGAGCACAC610140.15132222049771007No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG609940.15127261803909475No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT595310.14764419819138522No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATTGGAAGAGCACAC594460.1474333877422702No Hit
ACCGCCTGGGAATACCAGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT586890.1455559346836809No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAATAGCACACG584130.1448714207547897No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGTAGAGCACAC532580.13208638704669493No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGATGAGCACAC519620.12887214772842318No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCTTAATAGCACAC518920.1286985391232696No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACAC510120.1265160309441962No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG508250.1260522479561431Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGAA500650.12416735452876151Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGTAAGAGCACA497380.12335635433040129No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTC497330.12334395371574745No Hit
ATTGATCATCGACACTTCGAACGCACAGATCGGAAGAGCACACGTCTGAA497120.12329187113420138Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCTGAAGAGCACA481790.11948984268133828No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGTAAGAGCACACG454120.11262734253191088No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGTGCACACGT449630.11151376733599729No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAATATCACACGTC436290.10820528334635647No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT433130.10742156450023463No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAATAGCACACG420020.10417012333800142No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAATATCACACGT413360.10251836146611179No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT413250.10249108011387337Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)