FastQCFastQC Report
Thu 24 Aug 2023
SRR11005893_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11005893_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62704224
Total Bases3.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC28786064.590768877069589No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT20927793.337540705391713No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC19629843.1305450809821043No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT15822452.5233467525249975No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC13028762.077812174184629No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACACG7466861.1908065396040943No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCTGAAGAGCACAC7366541.17480761742622No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT6169650.9839289295725915No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT4914540.7837653807820028No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGTAAGAGCACAC4713630.7517244771261343No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAATAGCACAC3965430.6324023721272749No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT3890930.6205211948719754No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACG3855940.6149410285342181No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA3840390.6124611318050918Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCT3580450.5710061893119034Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC3512260.5601313238482946No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA3319340.5293646565181956No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGA3264110.5205566374603409Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG2775400.44261770945446993No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG2703460.43114479815586265No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA2662770.4246556021489079Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC2631560.41967826601282876No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAATAGCACACGT2456780.39180454573522827No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGATCACACGTC2455310.3915701117679089No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA2421180.38612709727497785Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC2219770.3540064541744429No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAATAGCACACGT2131420.3399164943018831No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT1997800.3186069251092239Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCTTAAGAGCACAC1792840.2859201319515572No Hit
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC1777350.2834498039557909Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGATCACACGT1755710.27999868079062745No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGATCACACGTC1728960.2757326205009729No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAATAGCACACGTC1674240.26700593567667785No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACG1672070.2666598664868255No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTC1645100.2623587208415178No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCTGAATAGCACAC1618140.2580591699851034No Hit
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA1613680.25734789413867876Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGATCACACGT1464200.23350898976120016No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACAC1461260.2330401218265615No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC1411400.22508850440442416No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG1375540.21936959143294718No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGT1336360.213121208548885No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACAC1264920.20172803669494418No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTG1245310.19860065567512647Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGTAATAGCACAC1220690.194674285419751No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAATAGCACACGTC1212480.19336496373832807No Hit
ATTGATCATCGACACTTCGAACGCACAGATCGGAAGAGCACACGTCTGAA1176610.18764445597795773Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAATAGCACACGT1171770.1868725781535866No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTGA1154280.18408329237915455Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACG1135400.18107233094854983No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG1133200.1807214773920175Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGT1125430.179482326421901No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAAGATCGGAAGAGCACAC1096790.1749148510314074No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACG1085390.17309679169301256No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGTAAGAGCACA997920.15914717324306574No Hit
TTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAAC975560.15558122527758259Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCTGAAGAGCACA967350.15427190359615964No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGATGAGCACAC917570.14633304448516896No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACAC899470.14344647658824386No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTC896350.14294890245352532No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAATAGCACACG895970.14288830047557882No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATTGGAAGAGCACAC895200.1427655017307925No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGTAGAGCACAC893450.14248641367445994No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT879930.14033025909067942No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT867430.13833677297401847Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA865000.13794923927293956Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACG848540.1353242167545204No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT819230.13064989050817374No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCTTAATAGCACAC797600.12720036213190358No Hit
CGCCACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGAA776330.12380824615579326Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACACGTCTG762740.12164092804975944Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACA742330.11838596391847542No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGATCACACGT739070.11786606273925024No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAATAGCACACGT714400.11393171853940813No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGTAAGAGCACACG684850.10921911735962157No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACA681480.10868167350256978No Hit
AGTGCGGTAACGCGACCGATCCCGGAGAAGATCGGAAGAGCACACGTCTG648860.1034794721325313Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCGCCTGGGAATACCAGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT629380.10037282336832683No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)