FastQCFastQC Report
Thu 24 Aug 2023
SRR11005898_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11005898_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45151514
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT21310234.7197154894960995No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC19879174.402769306916264No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC11689172.58887664320625No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT11005442.4374465051160854No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC9409152.0839057578445765No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACACG5631711.247291508320186No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT3839510.8503612968548518No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA2981300.6602879363026454Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT2962540.6561330368678224No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCTGAAGAGCACAC2875220.6367937075155442No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT2750950.609270820907578No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC2662370.5896524311454983No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG2548370.5644041083539303No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACG2537070.5619014237263451No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGATCACACGTC2426580.5374304835049385No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAATAGCACACGT2409380.5336210874346318No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCT2392710.5299290739176542Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA2171450.4809251800504408Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC2084180.4615969245239484No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA2074730.45950397145043687Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGCCACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGA2028340.44922967588639445Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGTAAGAGCACAC1918610.42492705781693163No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGATCACACGT1728130.38274021110344164No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAATAGCACACGTC1641300.36350940524386405No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT1622910.3594364521198558Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAATAGCACAC1602830.3549892036842884No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC1535550.340088263706949No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAATAGCACACGT1455510.3223612833890797No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACG1333790.29540316189618804No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTC1257970.2786108124746382No Hit
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA1237770.2741369868571849Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGATCACACGTC1206680.2672512819835897No Hit
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC1187820.2630742348971953Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACAC1151610.25505457026313666No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG1145110.2536149729109859No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGT1101170.24388329481044643No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGATCACACGT1005960.2227965157491729No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG993630.22006571031040068Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAAC946390.20960315970800003Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT857950.19001577665812047No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAATAGCACACGTC855950.18957282362668948No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACAC826750.1831057093677966No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC779400.17261879634866728No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG765370.1695114808331787No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGT757220.1677064472300973No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACG755280.16727678278960922No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTC718740.15918403090536454No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCTTAAGAGCACAC710910.15744986978731212No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAAGATCGGAAGAGCACAC695600.15405906433170768No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACACGTCTG687630.15229389650145508Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTGA681400.15091409780854745Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA676020.14972255415399802Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGCCACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTG666270.1475631581257718Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA666230.1475542990651432No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACAC663130.14686772186642513No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAATAGCACACG657010.1455122855902462No Hit
TTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACT645500.1429630908943607Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCTGAATAGCACAC637430.14117577541253654No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACG628150.13912047334669664No Hit
ATTGATCATCGACACTTCGAACGCACAGATCGGAAGAGCACACGTCTGAA618520.13698765450035627Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT615690.13636087596088142Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGTGCACACGT595530.13189590940405674No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT588580.13035664761983395No Hit
TTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTC587590.13013738586927562Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAATAGCACACGT556160.12317637898033719No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAATATCACACGTC541940.12002698292686266No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAATATCACACGT508040.11251892904410692No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGTAAGAGCACACG500280.11080027128215458No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGTAATAGCACAC499430.11061201624379638No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAATAGCACACGT495310.1096995329990485No Hit
CGCCACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGAA457830.10139859319003124Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGTGCACACGTC457720.10137423077330253No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)