FastQCFastQC Report
Thu 24 Aug 2023
SRR11005899_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11005899_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46971781
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT23293174.958971004314272No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC21544244.586634686046927No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC13066012.781672255518691No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT12052132.565823510077253No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC10191792.169768695804828No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACACG6198031.3195220338781706No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT4172380.888273748870625No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT3298510.7022322615359209No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA3174560.6758440775324231Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT3037280.646618019444483No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCTGAAGAGCACAC3025310.644069680900539No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC2879800.613091507005025No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG2806700.5975289717032446No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACG2774790.5907355311905248No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCT2583620.550036627310342Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAATAGCACACGT2348820.5000491678184398No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA2315460.4929470313250417Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGATCACACGTC2305560.4908393829052384No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC2254600.4799903158877455No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA2221690.4729839815952476Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGCCACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGA2159810.45981011450257764Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGTAAGAGCACAC2052430.4369495804300033No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT1744700.37143577757888296Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC1661700.3537655938572991No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAATAGCACAC1652760.35186232346608276No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGATCACACGT1619310.34474102653250466No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAATAGCACACGTC1556530.33137555503803445No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACG1478890.3148464819760613No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAATAGCACACGT1439440.30644782236381457No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTC1374620.29264804755859697No Hit
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA1322600.28157331313453926Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG1288410.2742944748039254No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACAC1279730.2724465567954513No Hit
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC1243260.2646823206469433Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGT1217820.25926630288938796No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGATCACACGTC1158080.24654802848544322No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG1070200.22783892311854215Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAAC1002320.2133876933472035Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGATCACACGT954680.20324543367857395No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT945220.20123145852187296No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACAC911930.19414422459305938No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACG856020.1822413333656648No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC847660.18046154136671974No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGT837680.17833686144453412No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG828700.17642507530212662No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAATAGCACACGTC806020.1715966443767589No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTC777480.16552065590189138No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAAGATCGGAAGAGCACAC770050.16393885511813996No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACACGTCTG748190.15928499709219032Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA739850.1575094629688408Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA739740.1574860446530652No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACAC737250.1569559391414177No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTGA727930.15497176911388563Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGCCACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTG710780.1513206407906909Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACG691120.1471351490802531No Hit
ATTGATCATCGACACTTCGAACGCACAGATCGGAAGAGCACACGTCTGAA673400.14336267130258484Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACT668400.14229820240369426Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT658170.14012029903656412Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT649370.13824683377451666No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCTGAATAGCACAC640140.1362818241871646No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAATAGCACACG639690.13618602198626448No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGTGCACACGT618070.13158325846746158No Hit
TTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTC600160.12777033087163547Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCTTAAGAGCACAC599240.1275744685942396No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAATAGCACACGT553780.11789631736552635No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAATATCACACGTC534500.11379172529140422No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAATATCACACGT501100.10668107304681507No Hit
CGCCACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGAA499470.10633405618577674Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAATAGCACACGT499360.10631063787000113No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGTGCACACGTC490030.1043243389046713No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGTAAGAGCACACG473790.10086694392107466No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)