FastQCFastQC Report
Thu 24 Aug 2023
SRR11005904_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11005904_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52463999
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC22416474.272733765491266No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT22108374.214007780840343No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC20211113.8523769413765048No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT10204431.9450347275281092No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC9361871.784436981252611No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACACG5809371.1073059832896077No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCTGAAGAGCACAC5621391.071475698983602No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACG4581560.8732769303384593No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT4405020.8396271889224457No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT3854720.7347362140655729No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCT3845000.7328835150366636Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGTAAGAGCACAC3715610.708220888766028No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA3232920.6162168461462497No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC3195960.6091720152708908No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGA3158810.6020909690852959Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAATAGCACAC2946430.5616098765174191No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA2853040.5438090985020032Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG2691530.5130241787325438No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT2490070.4746245134687503No Hit
ATTGATCATCGACACTTCGAACGCACAGATCGGAAGAGCACACGTCTGAA2355450.44896501313214804Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAATAGCACACGT2319330.44208029204941085No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGATCACACGTC2237560.42649436616526315No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC2236730.4263361624416011No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG2148110.4094445793200019No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC2020430.3851078908414892No Hit
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC1801320.3433440138636782Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT1732040.3301387681102998Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGATCACACGT1615650.30795403148738243No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACAC1525020.2906793285048667No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAATAGCACACGTC1508990.2876238999623342No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA1508790.2875857785831385Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA1406530.26809431740039485Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTC1384800.26395242955078585No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACG1326900.2529162902736408No Hit
CGCCACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGAA1302740.24831122766680444Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAATAGCACACGT1251750.2385921820408696No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTGA1229560.23436261501911054Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA1216340.23184279185427706Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATTGATCATCGACACTTCGAACGCAAGATCGGAAGAGCACACGTCTGAAC1200050.2287378055187901Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCTGAATAGCACAC1194480.22767612510819088No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCTTAAGAGCACAC1145900.2184164421015638No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT1108040.21120006501982436Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGATCACACGTC1085330.20687138241215658No Hit
TTGATCATCGACACTTCGAACGCACAGATCGGAAGAGCACACGTCTGAAC1067790.2035281374566967Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGCCACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTG1067440.20346142504310433Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGT1002980.19117490452834143No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG1002960.19117109239042185No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC965770.18408242192898794No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACG877680.1672918604622572No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACAC864230.1647281977113487No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGATCACACGT830810.15835811524775303No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGTAATAGCACAC816420.1556152820146249No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGTAAGAGCACA812950.15495387608558014No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGT809230.15424481843254076No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCTGAAGAGCACA803730.15319648050465998No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCT779710.14861810286326058Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAATAGCACACGTC772660.14727432424661338No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAATAGCACACGT764330.14568656880311392No Hit
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA758520.14457914273747985Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACG727130.13859599227272018No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT718390.13693008800186962No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAAGATCGGAAGAGCACAC713320.13596371103925953No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACA710310.13538998428236473No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACACGTCTG702360.13387465945933708Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT698090.1330607680135096No Hit
CCGCCACGCAGTTTTATCCGGTAAAGAGATCGGAAGAGCACACGTCTGAA688630.13125762677755465Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTC654610.12477318017637198No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGTAGAGCACAC634230.12088861163633371No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAATAGCACACG623220.11879002971161233No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACAC610180.11630451578805497No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACG609230.11612343923687557No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGT603930.11511322268819044No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACA573420.10929780629189172No Hit
TTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAAC566400.10795974588212384Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGTGCACACGT552780.10536367995889905No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG525570.1001772663193288Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)