FastQCFastQC Report
Fri 29 Sept 2023
SRR11114802.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11114802.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60676986
Total Bases3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGAACTGCCAGGCNTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC20450093.370320668201944RNA PCR Primer, Index 1 (100% over 33bp)
AGGGAACTGCCAGGCNTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG1879580.3097681878925232RNA PCR Primer, Index 6 (100% over 34bp)
AATGAACCGTGAGGCNTAACCTTGGAATTCTCGGGTGCCAAGGAACTCCA1826780.30106637135865644RNA PCR Primer, Index 1 (100% over 28bp)
AGGAAAAGCCTCTAANCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1623950.267638540912365RNA PCR Primer, Index 1 (100% over 33bp)
ACTGTTTCGGCAAGGNGGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGT1033140.17026883965528544RNA PCR Primer, Index 1 (100% over 30bp)
AGTCCGGATTGGAGTNTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC997350.1643703924252269RNA PCR Primer, Index 1 (100% over 31bp)
AGGGAACTGCCAGACNTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC937660.1545330547565431RNA PCR Primer, Index 1 (100% over 33bp)
AATGAACCGTGAGGCNTAACCTGGAATTCTCGGGTGCCAAGGAACTCCAG827320.13634823588633752RNA PCR Primer, Index 1 (100% over 29bp)
ATGCACCGAAGCTGCNGCAGCGACGCTTGGAATTCTCGGGTGCCAAGGAA802260.1322181691753773RNA PCR Primer, Index 1 (100% over 23bp)
ACCGAAGCTGCGGCANCGACGCTGGAATTCTCGGGTGCCAAGGAACTCCA797280.1313974296613876RNA PCR Primer, Index 1 (100% over 28bp)
AATGAACCGTGAGGCNTAACCTTTGGAATTCTCGGGTGCCAAGGAACTCC773340.12745194693750939RNA PCR Primer, Index 1 (100% over 27bp)
ATGCACCGAAGCTGCNGCAGCGACGCTGGAATTCTCGGGTGCCAAGGAAC749030.12344548557504159RNA PCR Primer, Index 1 (100% over 24bp)
ACCGAAGCTGCGGCANCGACGCTTGGAATTCTCGGGTGCCAAGGAACTCC675680.1113568824924824RNA PCR Primer, Index 1 (100% over 27bp)
ACAAGCAGTGGGAGCNCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT642060.1058160667373953RNA PCR Primer, Index 1 (100% over 30bp)
AGGTAGCGCCTCGTGNTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG640610.10557709639697661RNA PCR Primer, Index 6 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)