FastQCFastQC Report
Fri 29 Sept 2023
SRR11114803.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11114803.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32975473
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCTGAGGCGTGATGACGAGGCACTGGAATTCTCGGGTGCCAAGGAAC575189617.44295222088247RNA PCR Primer, Index 1 (100% over 24bp)
AGGCTGAGGCGTGATGACGAGGCTGGAATTCTCGGGTGCCAAGGAACTCC461838014.00550039115436RNA PCR Primer, Index 1 (100% over 27bp)
AAGGCTGAGGCGTGATGACGAGGCTGGAATTCTCGGGTGCCAAGGAACTC32705489.918123084997143RNA PCR Primer, Index 1 (100% over 26bp)
AGGCTGAGGCGTGATGACGAGGCACTGGAATTCTCGGGTGCCAAGGAACT25349477.68737115613171RNA PCR Primer, Index 1 (100% over 25bp)
AGGCTGAGGCGTGATGACGAGGTGGAATTCTCGGGTGCCAAGGAACTCCA8126482.4644013445993633RNA PCR Primer, Index 1 (100% over 28bp)
ATTCATCTCCGGGGGTAGAGCTGGAATTCTCGGGTGCCAAGGAACTCCAG5701651.7290578364107165RNA PCR Primer, Index 1 (100% over 29bp)
AAGGCTGAGGCGTGATGACGAGGTGGAATTCTCGGGTGCCAAGGAACTCC5562371.686820383137491RNA PCR Primer, Index 1 (100% over 27bp)
AAGGCTGAGGCGTGATGACGAGGCATGGAATTCTCGGGTGCCAAGGAACT5022281.5230350145394427RNA PCR Primer, Index 1 (100% over 25bp)
AGGCTGAGGCGTGATGACGAGGCATGGAATTCTCGGGTGCCAAGGAACTC4810201.4587205466317346RNA PCR Primer, Index 1 (100% over 26bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGTATGC4792121.453237683656577RNA PCR Primer, Index 12 (100% over 50bp)
ACGGTCCTAAGGTAGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC4501331.3650539599538116RNA PCR Primer, Index 1 (100% over 33bp)
ACGGTCCTAAGGTAGCGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA2499150.7578814714803332RNA PCR Primer, Index 1 (100% over 32bp)
AGGCTGAGGCGTGATGACGAGTGGAATTCTCGGGTGCCAAGGAACTCCAG1682860.5103368797772817RNA PCR Primer, Index 1 (100% over 29bp)
ACGGTCCTAAGGTAGCGTGGAATTCTCGGGTGCCACGGAACTCCAGTCAC1600690.4854183592756956RNA PCR Primer, Index 1 (96% over 33bp)
ACGATGTCGACTTGGAGGTTGTGCCTGGAATTCTCGGGTGCCAAGGAACT1349640.40928601691323735RNA PCR Primer, Index 1 (100% over 25bp)
AAGGCTGAGGCGTGATGACGAGTGGAATTCTCGGGTGCCAAGGAACTCCA1229980.3729984403862835RNA PCR Primer, Index 1 (100% over 28bp)
ACGGTCCTAAGGTAGCGATGGAATTCTCGGGTGCCCAGGAACTCCAGTCA828070.2511169437963786RNA PCR Primer, Index 1 (96% over 32bp)
AGGTAGCGCCTCGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC745260.22600433964965416RNA PCR Primer, Index 2 (100% over 34bp)
TGAGGCGAACCGGGGGAACTGATGGAATTCTCGGGTGCCAAGGAACTCCA624660.18943170276890342RNA PCR Primer, Index 1 (100% over 28bp)
AACGGTCCTAAGGTAGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA611090.18531652298058016RNA PCR Primer, Index 1 (100% over 32bp)
AGGGAACTGCCAGGCATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC588750.17854179074247092RNA PCR Primer, Index 1 (100% over 33bp)
AGGCTGAGGCGTGATGACGAGGCTGGAATTATCGGGTGCCAAGGAACTCC569370.1726646953631264RNA PCR Primer, Index 1 (96% over 27bp)
ACGGTGCTGAAGCAACAAATGCCCTGTGGAATTCTCGGGTGCCAAGGAAC528970.16041316526377045RNA PCR Primer, Index 1 (100% over 24bp)
GCGGGAATAGCTCAGTTGGTAGAGCACGTGGAATTCTCGGGTGCCAAGGA523250.15867854268534679RNA PCR Primer, Index 1 (100% over 22bp)
ATGGGGTGACTGCGTACCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT516540.1566436969683498RNA PCR Primer, Index 1 (100% over 30bp)
TGCTGAAGCAACAAATGCCCTGTGGAATTCTCGGGTGCCAAGGAACTCCA471000.14283343259397674RNA PCR Primer, Index 1 (100% over 28bp)
ATGGGGTGACTGCGTACCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC454880.1379449507820555RNA PCR Primer, Index 1 (100% over 31bp)
AGGGAACTGCCAGGCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC438460.13296549226147567RNA PCR Primer, Index 2 (100% over 34bp)
GGCTGAGGCGTGATGACGAGGCACTGGAATTCTCGGGTGCCAAGGAACTC403890.12248194286705151RNA PCR Primer, Index 1 (100% over 26bp)
AAGAACCTTACCTGGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC388570.11783606561155317RNA PCR Primer, Index 1 (100% over 33bp)
ATGTCGACTTGGAGGTTGTGCCTGGAATTCTCGGGTGCCAAGGAACTCCA383590.11632585224782066RNA PCR Primer, Index 1 (100% over 28bp)
TGCCCGGTAGCTAAATGCGGAAGTGGAATTCTCGGGTGCCAAGGAACTCC347000.10522972634842874RNA PCR Primer, Index 1 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)