FastQCFastQC Report
Fri 29 Sept 2023
SRR11114804.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11114804.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39340442
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGTGGAATTCTCGGGTGCCAA30419967.732490651731874No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGCTGGAATTCTCGGGTGCCA20526075.217549411366552No Hit
GCCCGGATGGTGGAATCGGTAGACACTGGAATTCTCGGGTGCCAAGGAAC9567772.432044357813773RNA PCR Primer, Index 1 (100% over 24bp)
GCGGGAATAGCTCAGTTGGTAGAGCACGACCTGGAATTCTCGGGTGCCAA8469212.1527999100772686No Hit
GCCGAGGTGGTGGAATTGGTAGACACTGGAATTCTCGGGTGCCAAGGAAC5560821.4135123342030576RNA PCR Primer, Index 1 (100% over 24bp)
ATCTCCGGGGGTAGAGCACTGTTTCGGTGGAATTCTCGGGTGCCAAGGAA4856581.2345006189813525RNA PCR Primer, Index 1 (100% over 23bp)
ATTCATCTCCGGGGGTAGAGCTGGAATTCTCGGGTGCCAAGGAACTCCAG4275401.086769691098031RNA PCR Primer, Index 1 (100% over 29bp)
ATCTCCGGGGGTAGAGCACTGTTTCGGCTGGAATTCTCGGGTGCCAAGGA3962551.007245927740212RNA PCR Primer, Index 1 (100% over 22bp)
TCGGACGCGGGTTCAACTCCCGCCAGCTCCTGGAATTCTCGGGTGCCAAG3073030.7811376394805122No Hit
GCGGGAATAGCTCAGTTGGTAGAGCACGTGGAATTCTCGGGTGCCAAGGA2700580.6864640717559808RNA PCR Primer, Index 1 (100% over 22bp)
ACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCTGGAATTCTCGGGT2563060.6515076775192307No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCTGGAATTCTCGGGTGCCA2507100.6372831296608208No Hit
GCGGGAATAGCTCAGTTGGTAGAGCACGACTGGAATTCTCGGGTGCCAAG2257950.573951355198297No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTGGAATTCTCGGGTGCCAAGGA2078190.5282579184036621RNA PCR Primer, Index 1 (100% over 22bp)
GCCCGGACGGTGGAATCGGTAGACACTGGAATTCTCGGGTGCCAAGGAAC1939170.49292023714425987RNA PCR Primer, Index 1 (100% over 24bp)
TTCGGACGCGGGTTCAACTCCCGCCAGCTCCTGGAATTCTCGGGTGCCAA1651570.419814805334419No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGATGGAATTCTCGGGTGCCAA1520970.3866174152288375No Hit
GCCGAGGTGGTGGAATTGGTAGACACGTGGAATTCTCGGGTGCCAAGGAA1498810.380984534947523RNA PCR Primer, Index 1 (100% over 23bp)
GGAGCGGTAGTTCAGTCGGTTAGAATACCTGCTGGAATTCTCGGGTGCCA1462270.37169638307571634No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGTATGC1448580.3682165035156443RNA PCR Primer, Index 12 (100% over 50bp)
AGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCTGGAATTCTCGGGTG1340990.34086805633754697No Hit
AACCCGATGCAAACTGCGAATACCGGAGATGGAATTCTCGGGTGCCAAGG1323740.33648325557704717Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCCATGCGAGAGTAGGGAACTGCCAGGCATGGAATTCTCGGGTGCCAA1314330.33409131498827593No Hit
AGGTAACCGTAGGGGAACCTGCGGTTGGATCTGGAATTCTCGGGTGCCAA1278510.3249861808873423No Hit
GCGGGAATAGCTCAGTTGGTAGAGCTGGAATTCTCGGGTGCCAAGGAACT1260270.3203497306918921RNA PCR Primer, Index 1 (100% over 25bp)
GCCCGGAAGGTGGAATCGGTAGACACTGGAATTCTCGGGTGCCAAGGAAC1236090.31420338388673924RNA PCR Primer, Index 1 (100% over 24bp)
CGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCTGGAATTCTCGGGTG1206680.30672761632927253No Hit
ACGTGCCGGTTCGAGTCCGGCCTTCGGCACCATGGAATTCTCGGGTGCCA1159290.2946814883269486No Hit
ATAACGTCGCAAGACCAAAGAGGGGGTGGAATTCTCGGGTGCCAAGGAAC1146960.2915473090007479RNA PCR Primer, Index 1 (100% over 24bp)
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCTGGAATTCTCGGGTGCCAA1144110.29082286365770876No Hit
GCGGGAGTGGCGAAATTGGTAGACGCTGGAATTCTCGGGTGCCAAGGAAC1143080.290561046568821RNA PCR Primer, Index 1 (100% over 24bp)
GCGAAGGTGGCGGAATTGGTAGACGCTGGAATTCTCGGGTGCCAAGGAAC1035680.2632608957469263RNA PCR Primer, Index 1 (100% over 24bp)
GCCGAGGTGGTGGAATTGGTAGACACGCTACCTGGAATTCTCGGGTGCCA945760.24040400969567144No Hit
GGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTGGAATTCTCGGGTGCCAA925560.235269344457289No Hit
TGGGGTATCGCCAAGCGGTAAGGCACCGGTGGAATTCTCGGGTGCCAAGG905680.2302160204504057Illumina Small RNA Adapter 2 (100% over 21bp)
AACCCGATGCAAACTGCGAATACCGGAGTGGAATTCTCGGGTGCCAAGGA868640.22080077290438171RNA PCR Primer, Index 1 (100% over 22bp)
GGAGAGATGCCGGAGCGGCTGAACGGTGGAATTCTCGGGTGCCAAGGAAC847670.21547038032770452RNA PCR Primer, Index 1 (100% over 24bp)
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCTGGAATTCTCGGGTGCC842880.21425280376870193No Hit
AGGCTGAGGCGTGATGACGAGGTGGAATTCTCGGGTGCCAAGGAACTCCA841140.21381051082242541RNA PCR Primer, Index 1 (100% over 28bp)
GCTGATATAGCTCAGTTGGTAGAGCGCACCCTGGAATTCTCGGGTGCCAA820540.2085741690446691No Hit
ACCATGCACCGAAGCTGCGGCAGCGACGCTGGAATTCTCGGGTGCCAAGG784810.19949191216509463Illumina Small RNA Adapter 2 (100% over 21bp)
ACGGTCCAGACTCCTACGGGAGGCTGGAATTCTCGGGTGCCAAGGAACTC774350.19683307066046693RNA PCR Primer, Index 1 (100% over 26bp)
GCCGACTTAGCTCAGTAGGTAGAGCAACTGTGGAATTCTCGGGTGCCAAG751960.19114172636901233No Hit
AGCGGTAGTTCAGTCGGTTAGAATACCTGCTGGAATTCTCGGGTGCCAAG742980.18885908806006807No Hit
GGGGCTATAGCTCAGCTGGGAGAGCGCTTGCTGGAATTCTCGGGTGCCAA739890.1880736367934046No Hit
AACCCGATGCAAACTGCGAATACCGGATGGAATTCTCGGGTGCCAAGGAA734250.1866399975882325RNA PCR Primer, Index 1 (100% over 23bp)
AACCCGATGCAAACTGCGAATTGGAATTCTCGGGTGCCAAGGAACTCCAG730770.18575541169567947RNA PCR Primer, Index 1 (100% over 29bp)
GCTGATATGGCTCAGTTGGTAGAGCGCACCCTGGAATTCTCGGGTGCCAA712310.18106303940357357No Hit
AGGCACGCTGATATGTAGGTGAAGCGACTGGAATTCTCGGGTGCCAAGGA694180.1764545502564511RNA PCR Primer, Index 1 (100% over 22bp)
AAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTTGGAATTCTCGGGTGCC692900.1761291853304546No Hit
AACCCGATGCAAACTGCGAATACCGTGGAATTCTCGGGTGCCAAGGAACT624040.1586255690772361RNA PCR Primer, Index 1 (100% over 25bp)
ACGGTCCAGACTCCTACGGGAGGCAGCTGGAATTCTCGGGTGCCAAGGAA588510.14959415046734859RNA PCR Primer, Index 1 (100% over 23bp)
ATCATCTCCGGGGGTAGAGCACTGTTTCGGTGGAATTCTCGGGTGCCAAG583650.1483587805139556No Hit
GCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTGGAATTCTCGGGTGCC583300.14826981354200341No Hit
GCCGAGGTGGTGGAATTGGTAGACACGCTGGAATTCTCGGGTGCCAAGGA579060.14719204222463997RNA PCR Primer, Index 1 (100% over 22bp)
ACGTACAAGCAGTGGGAGCCTCTGGAATTCTCGGGTGCCAAGGAACTCCA573550.14579144789476436RNA PCR Primer, Index 1 (100% over 28bp)
AACGTCGCAAGACCAAAGAGGGGGTGGAATTCTCGGGTGCCAAGGAACTC551240.14012043891118456RNA PCR Primer, Index 1 (100% over 26bp)
GCGAAGGTGGCGGAATTGGTAGACGCGTGGAATTCTCGGGTGCCAAGGAA551160.1401001036033098RNA PCR Primer, Index 1 (100% over 23bp)
ATTCATCTCCGGGGGTAGAGCACTGTTCGGTGGAATTCTCGGGTGCCAAG547250.13910621543092985No Hit
AGTCCGGTTCGACTCCGGAACGCGCCTGGAATTCTCGGGTGCCAAGGAAC545770.13873001223524636RNA PCR Primer, Index 1 (100% over 24bp)
GGCTACGTAGCTCAGTTGGTTAGAGCTGGAATTCTCGGGTGCCAAGGAAC537400.1366024306488473RNA PCR Primer, Index 1 (100% over 24bp)
TGGGGTATCGCCAAGCGGTAAGGCACCGGATGGAATTCTCGGGTGCCAAG535860.13621097597225776No Hit
ACAGGGGTTCGAATCCCCTAGGGGACTGGAATTCTCGGGTGCCAAGGAAC518870.13189226496235096RNA PCR Primer, Index 1 (100% over 24bp)
CCCCATGCGAGAGTAGGGAACTGCCAGGCATGGAATTCTCGGGTGCCAAG516360.13125424467777966No Hit
ATTCATCTCAGGGGGTAGAGCACTGTTTCGGTGGAATTCTCGGGTGCCAA514750.13084499660679969No Hit
GGTGGCTATAGCTCAGTTGGTAGAGCTGGAATTCTCGGGTGCCAAGGAAC510370.12973163850065536RNA PCR Primer, Index 1 (100% over 24bp)
CTTACGGGTTCAAGTCCCGTCCTCGGTACCATGGAATTCTCGGGTGCCAA501660.1275176318557885No Hit
AACCCGATGCAAACTGCGAATACCGGTGGAATTCTCGGGTGCCAAGGAAC496930.12631530677769204RNA PCR Primer, Index 1 (100% over 24bp)
AAGGCTGAGGCGTGATGACGAGGTGGAATTCTCGGGTGCCAAGGAACTCC490640.1247164431960373RNA PCR Primer, Index 1 (100% over 27bp)
ATTCATCTCCGGGGGTAGAGCACTGTTCGGCTGGAATTCTCGGGTGCCAA482350.12260919691751303No Hit
ACGGGCGGAGGGGAGGAAAGTCCGGGTGGAATTCTCGGGTGCCAAGGAAC480170.12205505977792522RNA PCR Primer, Index 1 (100% over 24bp)
GCCGAAGTGGCGAAATCGGTAGACGCTGGAATTCTCGGGTGCCAAGGAAC477880.12147296159000957RNA PCR Primer, Index 1 (100% over 24bp)
GGGTGATTAGCTCAGCTGGGAGAGCACCTGGAATTCTCGGGTGCCAAGGA477080.12126960851126178RNA PCR Primer, Index 1 (100% over 22bp)
ATCATCTCCGGGGGTAGAGCACTGTTTCGGCTGGAATTCTCGGGTGCCAA476270.12106371351902961No Hit
TCGGACGCGGGTTCAACTCCCGCCAGCTCCTGGCATTCTCGGGTGCCAAG470490.11959448752507662No Hit
AACCCGATGCAAACTGCGAATACCTGGAATTCTCGGGTGCCAAGGAACTC452480.11501650133976635RNA PCR Primer, Index 1 (100% over 26bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGTGGAATTCTCGGGTGCCAAG450230.1144445708057881No Hit
AGCAGGTACTGCAAACGCTGCAGAAATCTGGAATTCTCGGGTGCCAAGGA439950.11183148374387863RNA PCR Primer, Index 1 (100% over 22bp)
AAGTCGAACGGTAACAGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCCA429850.10926415112468742RNA PCR Primer, Index 1 (100% over 28bp)
CTCCGGGGGTAGAGCACTGTTTCGGTGGAATTCTCGGGTGCCAAGGAACT426370.1083795652321344RNA PCR Primer, Index 1 (100% over 25bp)
CATCTCCGGGGGTAGAGCACTGTTTCGGTGGAATTCTCGGGTGCCAAGGA425970.10827788869276049RNA PCR Primer, Index 1 (100% over 22bp)
GCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTGGAATTCTCGGGTGCCA418780.10645025289751446No Hit
ACCCGATGCAAACTGCGAATACCGGAGATGGAATTCTCGGGTGCCAAGGA417350.10608675926925275RNA PCR Primer, Index 1 (100% over 22bp)
GGGTGATTAGCTCAGCTGGGAGAGCACCTCCTGGAATTCTCGGGTGCCAA417110.10602575334562839No Hit
GCGGGAACAGCTCAGTTGGTAGAGCACGACCTGGAATTCTCGGGTGCCAA403550.10257891866085288No Hit
AGGGCTTACGGGTTCAAGTCCCGTCCTCGGTGGAATTCTCGGGTGCCAAG394900.10038016349689209No Hit
ATGCACCGAAGCTGCGGCAGCGACGCTGGAATTCTCGGGTGCCAAGGAAC394360.10024290016873731RNA PCR Primer, Index 1 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)