FastQCFastQC Report
Sun 1 Oct 2023
SRR11150215_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11150215_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences184044181
Total Bases18.5 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTC102634125.576602283339781No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG14577220.7920500349858929TruSeq Adapter, Index 6 (100% over 49bp)
TTGCAGTTAAAAAGCTCGTAGTTGAACCTTAGATCGGAAGAGCACACGTC7264080.3946921853508642No Hit
GGAATGAGTACAATCTAAATCCCTTAACGAAGATCGGAAGAGCACACGTC6003000.3261716815703073No Hit
ATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTCTGA5361940.2913398277992826Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGCAGTTAAAAAGCTCGTAGTTGAACCTTGAGATCGGAAGAGCACACGT4799350.260771624178653No Hit
ATTAAATCAGTTATAGTTTGTTTGATGAGATCGGAAGAGCACACGTCTGA4705800.2556886055528156Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAGAGGGTGAGAGCCCCGTCGTGCCCGGAGATCGGAAGAGCACACGTCT4058200.22050140232360835Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTAAATCAGTTATAGTTTGTTTGATGAGATCGGAAGAGCACACGTCTGAA4014860.21814653297840478Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAATCCTTTGCAGACGACTTAAATAAGATCGGAAGAGCACACGTCTGAA3901060.21196323506691037Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTGATGATTCATGATAACTCGACGGATCGCAGATCTGAAGAGCACACGTC3728650.20259537572665773No Hit
TTTATTAGCTGATGATTCATGATAACTCAGATCGGAAGAGCACACGTCTG3358330.18247412016791772Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTATTAGCTGATGATTCATGATAACTAGATCGGAAGAGCACACGTCTGA3276340.17801921159354667Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGATCCTTCGATGTCGGCTCTTCCTATAGATCGGAAGAGCACACGTCTG3075720.16711856812250966Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTGATGATTCATGATAACTCGACGGAGATCGGAAGAGCACACGTCTGAAC3016950.16392531312902525Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTATTAGCTGATGATTCATGATAACTCAGATCGGAAGAGCACACGTCTGA2554170.13878026385414488Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCATTAAATCAGTTATAGTTTGTTTGATGAGATCGGAAGAGCACACGTC2507600.1362498931710316No Hit
TGATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTCT2436590.1323915804759945Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGTCTCAAACAATGGAGATGAAGAAGAAGATCGGAAGAGCACACGTCTG2412940.13110656293990625Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAGAATCCTTTGCAGACGACTTAAATAAGATCGGAAGAGCACACGTCTGA2343420.12732920906638173Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTGATGATTCATGATAACTCGACGGATCGCAGATTGGAAGAGCACACGTC2186940.1188269027641792No Hit
TTGCAGTTAAAAAGCTCGTAGTTGAACCTAGATCGGAAGAGCACACGTCT2161730.11745712297201072Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTATTAGCTGATGATTCATGATAACTAGATCGGAAGAGCACACGTCTGAA2048130.11128469201642403Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)