FastQCFastQC Report
Sat 30 Sept 2023
SRR11150216_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11150216_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences144437049
Total Bases14.5 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTCTGAACTCCAG62934914.357255318889823Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTCTGAACTCCAGT34681312.401136705583067Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATAACTCGACGGATCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA29064222.0122413329006745Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTCTGAACT27321061.8915548461530807Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTCTGAACTC26819831.8568525309596986Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTCTGA25940361.7959630288486441Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTGATGATTCATGATAACTCGACGGAGATCGGAAGAGCACACGTCTGAAC22983541.5912496246028953Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GATAACTCGACGGATCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC20365171.4099685739217782Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTCTGAACTCC18154121.2568880440087087Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTCTGAA15992961.107261614019821Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTCTGAACTCCA14415590.9980534841860416Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTGATGATTCATGATAACTCGACGAGATCGGAAGAGCACACGTCTGAACT11001460.7616785358166657Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCTCTCAGGCGACGCCAAGATCGGAAGAGCACACGTCTGAACTCCAGTC9901290.6855090206114638Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTCTGAAC7845920.5432068886979268Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTGATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTC7365450.5099418778626529No Hit
TAACTCGACGGATCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC6325370.43793265258417186Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCGATGTCGGCTCTTCCTATAGATCGGAAGAGCACACGTCTGAACTCCAG4207370.29129437558641896Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGATGTCGGCTCTTCCTATAGATCGGAAGAGCACACGTCTGAACTCCAGT3919550.27136735533831075Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTCTG3169080.21940907972995213Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGATTCATGATAACTCGACGGAGATCGGAAGAGCACACGTCTGAACTCC3047900.2110192655625358Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCCACAGACGGCGCCAAGATCGGAAGAGCACACGTCTGAACTCCAGTC2768260.1916585820027381Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATCCCACTCTTGACACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTC2534070.17544459801307627Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCGATGTCGGCTCTTCCTAAGATCGGAAGAGCACACGTCTGAACTCCAGT2345820.16241123840739782Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AACGGGTGAGTAAACCCGTAAGGCGCAGATCGGAAGAGCACACGTCTGAA2293130.15876328240408732Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTGATGATTCATGATAACTCAGATCGGAAGAGCACACGTCTGAACTCCAG2164980.14989090506826958Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTCTCAGGCGACGCCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2138520.14805896512050729Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCTCTCAGGCGACGCCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1936240.13405424808976815Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATCCCACTGTCAACACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTC1888780.13076838754854372Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATGATAACTCGACGGATCGCAAGATCGGAAGAGCACACGTCTGAACTCCA1861780.12889906107123528Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTGATGATTCATGATAACTCGACAGATCGGAAGAGCACACGTCTGAACTC1826550.1264599361899176Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCCGCTCACGCCCGGTCGTAAGATCGGAAGAGCACACGTCTGAACTCCAG1789220.1238754192492537Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATAACTCGACGGATCGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTC1761820.12197839904635548Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AACTCGACGGATCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG1734110.12005991620612522Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGATGTCGGCTCTTCCTAAGATCGGAAGAGCACACGTCTGAACTCCAGTC1557810.10785390665244067Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTGATCCTTCGATGTCGGCTCTTCCTATAGATCGGAAGAGCACACGTCTG1538290.10650245284366062Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGAGAGTGACACCCAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGT1507970.1044032684439572Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTGATCCTTCGATGTCGGCTCTTCCTAAGATCGGAAGAGCACACGTCTGA1494720.10348591378379655Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCGCTCACGCCCGGTCGTACTCAGATCGGAAGAGCACACGTCTGAACTC1491930.10329275004780802Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GATGTCGGCTCTTCCTATAGATCGGAAGAGCACACGTCTGAACTCCAGTC1457220.10088962700975704Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)