FastQCFastQC Report
Sat 30 Sept 2023
SRR11150217_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11150217_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences172400235
Total Bases17.4 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGCCGGAGGCACGACCCGGCCAATTAAGACCAGATCGGAAGAGCACAC70818414.107790804345481No Hit
AGGAGCGTATCGCCGACCGAAGGGACAGATCGGAAGAGCACACGTCTGAA11394850.6609532753827163Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGCCGACGCCCGGAGCACGCTGATGCCGAGGCAGATCGGAAGAGCACAC8250960.47859331514252285No Hit
CTGATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTC5134840.29784414156976063No Hit
ACGATCACGGCAGCGACGTCTCCACAGATCGGAAGAGCACACGTCTGAAC5102750.29598277519749316Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACGGCAGCGACGTCTCCACAAGCGTATCAGATCGGAAGAGCACACGTCTG4597410.2666707501877825Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCCGACGGGTTCGGAACTGGGACCCCCGAGCCCAGATCGGAAGAGCACA4260640.24713655407720297No Hit
AGCGCCGGAGGCACGACCCGGCCAATTAAGACAGATCGGAAGAGCACACG3875780.224812918613481No Hit
AGACCACGATCACGGCAGCGACGTCTCCACAGATCGGAAGAGCACACGTC3394950.19692258540134822No Hit
TTAAATCAGTTATAGTTTGTTTGATGAGATCGGAAGAGCACACGTCTGAA3378470.19596667023104694Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TAGAATCCTTTGCAGACGACTTAAATAGATCGGAAGAGCACACGTCTGAA2836120.16450789640744978Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAATCCTTTGCAGACGACTTAAATAGATCGGAAGAGCACACGTCTGAAC2807130.1628263441752269Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACGTGTGTCGCCCAGGGCATAAGGGGCAGATCGGAAGAGCACACGTCTGA2780300.16127008179542213Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAATCCTTTGCAGACGACTTAAATAAGATCGGAAGAGCACACGTCTGAA2711330.15726950720223787Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGAAAAGCCCAAAGCCAATCCCAGGGAACAGATCGGAAGAGCACACGTC2685970.15579851152755098No Hit
AGCCCCAGGTGGCGAAGAGCCGACAGATCGGAAGAGCACACGTCTGAACT2614660.15166220626091373Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGAGAGTCTCCGGACTCCCTAACGTTGCAGATCGGAAGAGCACACGTCT2436610.14133449412061416Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAAAAGATAACTCTTCCCGAGGCCCCCGCAGATCGGAAGAGCACACGTC2298420.13331884379391942No Hit
ACGATCACGGCAGCGACGTCTCCAGATCGGAAGAGCACACGTCTGAACTC2296200.133190073667823Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGACCACGATCACGGCAGCGACGTCTCCAGATCGGAAGAGCACACGTCTG2003100.11618893674941917Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCCGCTGGACCCTACCTCCGGCTGAGCCGTTTCCAGATCGGAAGAGCAC1974710.11454218725397908No Hit
ACGCGGCGACCCGCTCTCGCCGCGGAAGCAGATCGGAAGAGCACACGTCT1794690.10410020612790928Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAAATCAGTTATAGTTTGTTTGATGAGATCGGAAGAGCACACGTCTGAAC1757600.10194881694911843Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)