FastQCFastQC Report
Sat 30 Sept 2023
SRR11150218_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11150218_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96391163
Total Bases9.7 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGCCGGAGGCACGACCCGGCCAATTAAGACCAGATCGGAAGAGCACAC43814174.545455064174296No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20846222.1626692065122195No Hit
ACGCGGCGACCCGCTCTCGCCGCGGAAGCAGATCGGAAGAGCACACGTCT14458371.4999684151544057Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG12661521.3135560984983654TruSeq Adapter, Index 8 (100% over 49bp)
AGAGAGGGTGAGAGCCCCGTCGTGCCCGGAGATCGGAAGAGCACACGTCT8589920.8911522314550764Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGAGCGTATCGCCGACCGAAGGGACAGATCGGAAGAGCACACGTCTGAA8113190.8416943781454322Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGCCGACGCCCGGAGCACGCTGATGCCGAGGCAGATCGGAAGAGCACAC7876480.817137147727951No Hit
AGAAAAGATAACTCTTCCCGAGGCCCCCGCAGATCGGAAGAGCACACGTC7652130.7938621925331475No Hit
GGGAGAGGTGATCCAGCCGCACCTTCCAGATCGGAAGAGCACACGTCTGA7400720.76777992604986Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGAGAGTCTCCGGACTCCCTAACGTTGCCGTCAGATCGGAAGAGCACAC6204010.6436285035797317No Hit
AGTAAGCGCGAGTCATCAGCTCGCGTTGACTAGATCGGAAGAGCACACGT5869960.6089728370639121No Hit
AGACCACGATCACGGCAGCGACGTCTCCACAGATCGGAAGAGCACACGTC5489840.5695376867690661No Hit
AGGAAAAGCCCAAAGCCAATCCCAGGGAACAGATCGGAAGAGCACACGTC4970150.515622993365066No Hit
GGGAGAGTCTCCGGACTCCCTAACGTTGCAGATCGGAAGAGCACACGTCT4895900.5079200050734941Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGGCAGCGACGTCTCCACAAGCGTATCAGATCGGAAGAGCACACGTCTG4341070.4503597492645669Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCGCCGGAGGCACGACCCGGCCAATTAAGACAGATCGGAAGAGCACACG4232960.4391439908241381No Hit
AGACCACGATCACGGCAGCGACGTCTCCAGATCGGAAGAGCACACGTCTG3657340.37942689829357074Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC3544650.36773599256189077No Hit
AGCCGCTGGACCCTACCTCCGGCTGAGCCGTTTCCAGATCGGAAGAGCAC3519170.3650925967144934No Hit
AGCCGACGGGTTCGGAACTGGGACCCCCGAGCCCAGATCGGAAGAGCACA3233210.3354259767568112No Hit
CTGATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTC3186800.33061122003476606No Hit
AGAAAAGATAACTCTTCCCGAGGCCCCCGCCAGATCGGAAGAGCACACGT3041330.3155195876202884No Hit
AATCGGGCGGTAAATTCCGTCCAAGGCTAAATAGATCGGAAGAGCACACG2983960.30956779720564215No Hit
AGTTGGGCACCGTAACCCGGCTTCCGGTTCAGATCGGAAGAGCACACGTC2975100.3086486258081563No Hit
AGCTCCCATAGTGTGACGGGCGGTGTGTACAGATCGGAAGAGCACACGTC2839950.29462763095824457No Hit
ATTCCCAAACAACCCGACTCGTAGACAGATCGGAAGAGCACACGTCTGAA2818450.2923971360320655Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCCCCAGGTGGCGAAGAGCCGACAGATCGGAAGAGCACACGTCTGAACT2784440.2888688042907004Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACGATCACGGCAGCGACGTCTCCACAGATCGGAAGAGCACACGTCTGAAC2669800.27697559785641346Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGAGAATCTTAGCGACAAAGGGCTGAATCTCAGATCGGAAGAGCACACG2534600.262949415808999No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACA2423520.2514255378369073No Hit
GGGAGAGTCTCCGGACTCCCTAACGTTGCCAGATCGGAAGAGCACACGTC2286830.23724477730391116No Hit
AATGGGCGAACAGCCCAACCCTTGGAACAGATCGGAAGAGCACACGTCTG2188740.22706853324303183Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGCGCGAGTCATCAGCTCGCGTTGACTACAGATCGGAAGAGCACACGTC2120850.22002535647380872No Hit
ACGATCACGGCAGCGACGTCTCCACAAGCGTATCAGATCGGAAGAGCACA1953090.20262127141260863No Hit
AGGCCGACGCCCGGAGCACGCTGATGCCAGATCGGAAGAGCACACGTCTG1945540.20183800458969461Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTGGATCGTGGCAGCAAGGCCACTCTGCCACTTACAGATCGGAAGAGCA1667880.17303245941746756No Hit
ACGTGTGTCGCCCAGGGCATAAGGGGCAGATCGGAAGAGCACACGTCTGA1662450.17246912976866977Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGCGGCGACCCGCTCTCGCCGCGGAAGCAGCTCGAGCAGATCGGAAGAG1648320.1710032277543949No Hit
AGCGGGTAATCCCGCCTGACCTGGGGTCAGATCGGAAGAGCACACGTCTG1537790.15953640895483334Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCGCCTCGTGGTGCGACAGGGTCCGGGCACAGATCGGAAGAGCACACGT1518270.15751132704976287No Hit
AACACCCTTTGTGGGTTCTAGGTTAGCGCGCAGATCGGAAGAGCACACGT1517720.15745426787723268No Hit
AGGCCGACGCCCGGAGCACGCTGATGCAGATCGGAAGAGCACACGTCTGA1502550.15588047215490075Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCGCCGTAGCGCCTGGTACTCGAACAGATCGGAAGAGCACACGTCTGAA1499850.1556003634897527Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGT1462710.1517473131847159TruSeq Adapter, Index 8 (100% over 50bp)
ACAGGCCGACGCCCGGAGCACGCTGATGCCGAGGCAGATCGGAAGAGCAC1455060.15095367196679638No Hit
AGGGACACCTTGCGTCCTTGAACCGATAACCAGATCGGAAGAGCACACGT1427150.14805817832076576No Hit
ATACTACAGCCCCAGGTGGCGAAGAGCCGACAGATCGGAAGAGCACACGT1419290.14724275087333472No Hit
AAGGGCCGCCGGGGGCGCACCGGACACCAGATCGGAAGAGCACACGTCTG1386370.14382750003752937Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGACGGGTCGAATGGGGAGCCCACAGATCGGAAGAGCACACGTCTGAAC1350010.14005537001353538Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATGCGCGTCACTAATTAGATGACGAGGCAGATCGGAAGAGCACACGTCTG1347320.13977629878788783Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTTGGCTACCCAGCGTTTACCGTGGGCAGATCGGAAGAGCACACGTCTG1266800.1314228359294721Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAATTCCGAAGGTCTAAAGGATCGATAGGCCAGATCGGAAGAGCACACGT1258230.1305337502775021No Hit
AACGAGAACAGAAATCTCGTGTGGAACAGATCGGAAGAGCACACGTCTGA1244560.12911557048025243Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCGACCGCCGCGCCCTCCTACTCAGATCGGAAGAGCACACGTCTGAACT1199880.12448029079180215Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGGGGTTGTACCCCGCCTCCGATTCAGATCGGAAGAGCACACGTCTGAAC1190620.12351962181429432Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCCTCCGTGGACGAACCTTGCGGAGGAACAGATCGGAAGAGCACACGTC1167770.12114907255554122No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAA1162690.12062205328926263No Hit
AGCCGGAGGTAGGGTCCAGCGGCTGGAAGAGATCGGAAGAGCACACGTCT1070090.11101536351418438Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATACCCCGTCGCGTATTTAAGTCGTCTGCAGATCGGAAGAGCACACGTC990700.10277913131933059No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)