FastQCFastQC Report
Sat 30 Sept 2023
SRR11150219_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11150219_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133783283
Total Bases13.5 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG25176211.8818651654706364TruSeq Adapter, Index 2 (100% over 49bp)
AGCGCCGGAGGCACGACCCGGCCAATTAAGACCAGATCGGAAGAGCACAC13144690.9825360616991288No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6568440.49097614086806346No Hit
GGGAGAGGTGATCCAGCCGCACCTTCCAGATCGGAAGAGCACACGTCTGA5169630.3864182343320129Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGACGGGTCGAATGGGGAGCCCACAGATCGGAAGAGCACACGTCTGAAC4665330.34872294171462365Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACGCGGCGACCCGCTCTCGCCGCGGAAGCAGATCGGAAGAGCACACGTCT4554310.34042444600496163Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAAAAGATAACTCTTCCCGAGGCCCCCGCAGATCGGAAGAGCACACGTC4146180.3099176449422309No Hit
GGGAGAGTCTCCGGACTCCCTAACGTTGCAGATCGGAAGAGCACACGTCT4111010.3072887664148592Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGACCACGATCACGGCAGCGACGTCTCCACAGATCGGAAGAGCACACGTC4086740.30547463841203537No Hit
GGGAGAGTCTCCGGACTCCCTAACGTTGAGATCGGAAGAGCACACGTCTG3277070.2449536239890301Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGGCAGCGACGTCTCCACAAGCGTATCAGATCGGAAGAGCACACGTCTG3038410.22711432488915673Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGAGAGTCTCCGGACTCCCTAACGTAGATCGGAAGAGCACACGTCTGAA2883480.21553365527739368Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATGGGCGAACAGCCCAACCCTTGGAACAGATCGGAAGAGCACACGTCTG2822440.210971052339925Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGAGAGTCTCCGGACTCCCTAACGTTAGATCGGAAGAGCACACGTCTGA2688020.20092345917389395Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTCCTCTACGCCTAGGACACCAGAATACAGATCGGAAGAGCACACGTCT2533780.1893943655127674Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGTCTCAAACAATGGAGATGAAGAAGAAGATCGGAAGAGCACACGTCTG2485270.1857683519397562Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTGATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTC2409720.18012115908382964No Hit
AGGAAAAGCCCAAAGCCAATCCCAGGGAACAGATCGGAAGAGCACACGTC2191220.1637887747155973No Hit
GGGAGAGTCTCCGGACTCCCTAACGAGATCGGAAGAGCACACGTCTGAAC2165070.16183412093422764Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGAGAGTCTCCGGACTCCCTAACGTTGCCAGATCGGAAGAGCACACGTC2120290.15848691648567184No Hit
AAAGGCGGACCGGCCGACCCATCCCAGATCGGAAGAGCACACGTCTGAAC2109340.15766842857339655Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACGATCACGGCAGCGACGTCTCCACAGATCGGAAGAGCACACGTCTGAAC1954630.14610420346763353Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGACCACGATCACGGCAGCGACGTCTCCAGATCGGAAGAGCACACGTCTG1935150.14464811720908358Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTCAACTAGCAATGGAGATGAAGAAGAAGATCGGAAGAGCACACGTCTG1912530.14295732300126018Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCGCCGGAGGCACGACCCGGCCAATTAAGACAGATCGGAAGAGCACACG1876780.14028509077625193No Hit
ATCTTCGGCGCAAGAGCGCTCGATCAGATCGGAAGAGCACACGTCTGAAC1866330.13950397674124948Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTTTCGGCCTGATCTTAGGCCCTGACTCAGATCGGAAGAGCACACGTCTG1714770.12817520706230537Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGAGCGTATCGCCGACCGAAGGGACAGATCGGAAGAGCACACGTCTGAA1708090.12767589206194022Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC1573380.11760662204709088No Hit
GGGAGAGTCTCCGGACTCCCTAACAGATCGGAAGAGCACACGTCTGAACT1533160.1146002673592634Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)