FastQCFastQC Report
Sat 30 Sept 2023
SRR11150220_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11150220_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81644327
Total Bases8.2 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9946361.2182548825468302No Hit
AGCGCCGGAGGCACGACCCGGCCAATTAAGACCAGATCGGAAGAGCACAC4814490.5896906958397734No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG4067950.4982526220101TruSeq Adapter, Index 9 (100% over 49bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC2453430.30050220145730394No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACA1805690.221165397076517No Hit
ACGCGGCGACCCGCTCTCGCCGCGGAAGCAGATCGGAAGAGCACACGTCT1607210.1968550735925596Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGAGAGGTGATCCAGCCGCACCTTCCAGATCGGAAGAGCACACGTCTGA1271070.15568381131979936Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAAAAGATAACTCTTCCCGAGGCCCCCGCAGATCGGAAGAGCACACGTC1170830.14340616709352016No Hit
GGGAGAGTCTCCGGACTCCCTAACGTTGAGATCGGAAGAGCACACGTCTG1058950.12970282674998349Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGAGAGTCTCCGGACTCCCTAACGTTGCAGATCGGAAGAGCACACGTCT1053610.12904877028381898Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAA1027320.1258287057715596No Hit
AGACCACGATCACGGCAGCGACGTCTCCACAGATCGGAAGAGCACACGTC964590.11814537953139106No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA942680.11546178830036777No Hit
AAGACGGGTCGAATGGGGAGCCCACAGATCGGAAGAGCACACGTCTGAAC892190.109277647668037Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAA835640.10235126318084538No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)