FastQCFastQC Report
Sat 26 Aug 2023
SRR11179726.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11179726.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89849690
Total Bases9 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA8861660.9862760795279316Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCCACAGAATTCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT5879350.6543539549218256No Hit
CTGTAGGCACCATCAACTGTAGGCACCATCAATAGATCGGAAGAGCACAC5499380.6120644378405757No Hit
TCCACAGAATTCGCACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC4470640.4975687729139633No Hit
TGGTCCTATAGTGTAGTGGTTATCACTTTCGGCTGTAGGCACCATCAATA4057670.4516064551808693No Hit
TTGGAAGAGATGTATTTATTAGATCTGTAGGCACCATCAATAGATCGGAA3974980.44240330712326337No Hit
AAATGGTCAGAATGGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA3619320.4028194198555387No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGAGCCTGTAGGCACCATCA3197220.3558409606087678No Hit
TCGTTGGGCCCCCGGTTCGATTCCGGGCTTGCGCACCACTGTAGGCACCA2795570.3111385247962458No Hit
CCCCCCATCGTGAGTGCCACTGTAGGCACCATCAATAGATCGGAAGAGCA2790140.3105341821435333No Hit
TCCCGCTAGAGACCACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC2698100.30029040723457145No Hit
AAATTCTAGAGCTAATACATGCTCTGTAGGCACCATCAATAGATCGGAAG2623450.29198208697214206No Hit
AATTCCCCGTCGCGGAGCCTGTAGGCACCATCAATAGATCGGAAGAGCAC2602980.28970383759810414No Hit
ACCTCCCCTGGAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2562460.2851940835855972No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGCTGTAGGCACCATCAATA2471270.27504491111766777No Hit
TTTGGAAGAGATGTATTTATTAGATCTGTAGGCACCATCAATAGATCGGA2372340.2640342999513966No Hit
GCCCCCTATGAGGAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC2356880.26231364849450234No Hit
AGATCGGGGTTCAATTCCCCGTCGCGGAGCCTGTAGGCACCATCAATAGA2331050.2594388472570134No Hit
TCCTGAGTGCGAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2310020.25709827156888354No Hit
TAAGGTTAGGGGTTCGAGCCCCCTACAGGGCCTGTAGGCACCATCAATAG2197280.24455064897831036No Hit
CGTTGGGCCCCCGGTTCGATTCCGGGCTTGCGCACCACTGTAGGCACCAT2195890.24439594616297508No Hit
GCCCCCTATGAGGAGCCACTGTAGGCACCATCAATAGATCGGAAGAGCAC2141320.23832246944869817No Hit
GCGTAGTCGGTAGCGCTCTCCCCTGTAGGCACCATCAATAGATCGGAAGA2117410.23566135843095282No Hit
ACCTCCCCTGGAGCACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC2088480.23244153652616945No Hit
TTGGAAGAGATGTATTTATTAGATACTGTAGGCACCATCAATAGATCGGA1983320.22073754511562588No Hit
ATCCGATTGGAAGCACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC1968050.2190380400867271No Hit
GTTTAGTGGTAAAATCCAACGTTGCCATCGTTGGGCCCCCGGTTCGATTC1951190.217161572844603No Hit
CGTGGAGACCGGGGTTCGACTCCCCGTATCGGAGCCACTGTAGGCACCAT1910350.2126162037954722No Hit
CCGTGGAGACCGGGGTTCGACTCCCCGTATCGGAGCCACTGTAGGCACCA1891710.2105416279121275No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCCTGTAGGCACCATCAATAGAT1876470.20884546179291216No Hit
CCGTGGAGACCGGGGTTCGACTCCCCGTATCGGAGCCTGTAGGCACCATC1836990.20445145664943304No Hit
ATGTATTTATTAGATACTGTAGGCACCATCAATAGATCGGAAGAGCACAC1834550.2041798919951755No Hit
TGATGTATTTATTAGATCTGTAGGCACCATCAATAGATCGGAAGAGCACA1814300.20192612795881656No Hit
TCGTGGAGACCGGGGTTCGACTCCCCGTATCGGAGCCACTGTAGGCACCA1782490.19838577072441763No Hit
ATTTGATAGTTCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1777970.19788270833210445No Hit
TCCACAGAATTCGCACCCTGTAGGCACCATCAATAGATCGGAAGAGCACA1774100.19745198898293362No Hit
ATGGGTTCCCCCCCATCGTGAGTGCCACTGTAGGCACCATCAATAGATCG1725640.1920585368741951No Hit
TCCCGCTAGAGACCACCCTGTAGGCACCATCAATAGATCGGAAGAGCACA1716030.1909889728055823No Hit
TGGTCCTATAGTGTAGTGGTTATCACTTTCGGTCTGTAGGCACCATCAAT1707300.19001735008768533No Hit
AAAATCAGTTATCGTTTATTTGATAGTTCTGTAGGCACCATCAATAGATC1698050.18898785293527445No Hit
ATCCGGGTAGGACCTCCACTGTAGGCACCATCAATAGATCGGAAGAGCAC1697020.18887321703614113No Hit
ATTTGATAGTTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1690210.18811528453798784Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCCGATATAGTGTAACGGCTATCACATCACGCTTTCACCGTGGAGACCG1643770.182946652347938No Hit
TCTAGAGCTAATACATGCTCTGTAGGCACCATCAATAGATCGGAAGAGCA1642900.18284982396711663No Hit
TGGGAGAGGTCTCCGGTTCGATTCCGGACTCGTCCACCCTGTAGGCACCA1639110.1824280083771018No Hit
CGTGGAGACCGGGGTTCGACTCCCCGTATCGGAGCCTGTAGGCACCATCA1625840.18095109732710263No Hit
AAAATCAGTTATCGTTTATTTGATAGTTCCTGTAGGCACCATCAATAGAT1612990.17952093101267239No Hit
TCCCGCTAGAGACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1594190.1774285476110157No Hit
TCGTGGAGACCGGGGTTCGACTCCCCGTATCGGAGCCTGTAGGCACCATC1577670.17558992134530457No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA1577390.1755587581882586No Hit
TATCGTTGGGCCCCCGGTTCGATTCCGGGCTTGCGCACCACTGTAGGCAC1562000.17384589752062585No Hit
TCCGATATAGTGTAACGGCTATCACATCACGCTTTCACCGTGGAGACCGG1561780.17382141218294686No Hit
GTCTAGTCGGTTATGGCATCTGCCTGTAGGCACCATCAATAGATCGGAAG1559060.17351868437164336No Hit
TCACCGTGGAGACCGGGGTTCGACTCCCCGTATCGGAGCCACTGTAGGCA1556230.17320371389150035No Hit
ATCCGATTGGAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1553260.17287316183283435No Hit
CCCCCCATCGTGAGTGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA1534500.17078523031075565No Hit
TCCTGGGCGAAATCACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC1497010.16661270617628174No Hit
ATTTATTAGATACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1494720.16635783607044163Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGTGGAGACCGGGGTTCGACTCCCCGTATCGGAGCCTGTAGGCACCAT1490450.1658825979254909No Hit
TCACCGTGGAGACCGGGGTTCGACTCCCCGTATCGGAGCCTGTAGGCACC1457740.16224207340058713No Hit
AGAGCTAATACATGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1400160.15583359274806624No Hit
ACCTCCCCTGGAGCACCCTGTAGGCACCATCAATAGATCGGAAGAGCACA1373940.15291538568469185No Hit
CCCTCACTGGGGTCGCCACTGTAGGCACCATCAATAGATCGGAAGAGCAC1356880.15101665904467784No Hit
TTTGGAAGAGATGTATTTATTAGATACTGTAGGCACCATCAATAGATCGG1344110.14959539648940356No Hit
AAAAATCTCGACCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1341430.14929712055767805No Hit
TCGTTGGGCCCCCGGTTCGATTCCGGGCTTGCGCCTGTAGGCACCATCAA1296550.1443021116711699No Hit
ATGTACACGGAGTCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1284560.1429676607676665No Hit
TCTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCA1281290.1426037196121656No Hit
TCCTGGGCGAAATCACCCTGTAGGCACCATCAATAGATCGGAAGAGCACA1252120.13935718642991424No Hit
GTCTAGTGGTATGATTCTCGCTTTGGGTGCGAGAGGCCCTGGGTTCATTT1246880.13877399020519715No Hit
TTGGGAGAGGTCTCCGGTTCGATTCCGGACTCGTCCACCCTGTAGGCACC1243550.13840337123032923No Hit
AAAAATCCAACGTTGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1237850.13776897839046523No Hit
ATCCGGGTAGGACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1234510.1373972464457028No Hit
TGATGTATTTATTAGATACTGTAGGCACCATCAATAGATCGGAAGAGCAC1230600.136962075216954No Hit
TTCCGATATAGTGTAACGGCTATCACATCACGCCTGTAGGCACCATCAAT1184050.13178120035806468No Hit
TAGAGATGTATTTATTAGATCTGTAGGCACCATCAATAGATCGGAAGAGC1181760.1315263302522246No Hit
AAAGATTGGAAATCTTTTGGGCCTGTAGGCACCATCAATAGATCGGAAGA1167470.1299358962729866No Hit
TCCACAGAGTTCGCACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC1166510.12982905116311474No Hit
ATTTGATAGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG1144020.12732598187038818Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCACAGAATTCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1142920.1272035551819934No Hit
TTGCCATCGTTGGGCCCCCGGTTCGATTCCGGGCTTGCGCACCACTGTAG1132030.12599153096688478No Hit
ATTAAATCAGTTATCGTTTATTTGATAGTTCCTGTAGGCACCATCAATAG1126610.12538830128406675No Hit
AATTCCCCGTCGCGGAGCCACTGTAGGCACCATCAATAGATCGGAAGAGC1121770.12484962385512961No Hit
CCCTCACTGGGGTCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1115840.12418963270769215No Hit
ATCCGGGTAGGACCTCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA1110990.12364984230886049No Hit
AAGTATAAGCAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1101770.12262368406613312No Hit
ACTGTAGGCACCATCAACTGTAGGCACCATCAATAGATCGGAAGAGCACA1098770.12228979309778364No Hit
TCCGATATAGTGTAACGGCTATCACATCACGCCTGTAGGCACCATCAATA1085940.1208618527231424No Hit
TATCGTTGGGCCCCCGGTTCGATTCCGGGCTTGCGCCTGTAGGCACCATC1066710.11872161161602228No Hit
ACCGTGGAGACCGGGGTTCGACTCCCCGTATCGGAGCCACTGTAGGCACC1053450.1172458135359176No Hit
TATGACTCTTAATCATAAGGTTAGGGGTTCGAGCCCCCTACAGGGCCTGT1052940.11718905207129819No Hit
GCCCCCTATGAGGAGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA1049940.1168551611029487No Hit
ATTAAATCAGTTATCGTTTATTTGATAGTTCTGTAGGCACCATCAATAGA1040840.11584235849895531No Hit
TTTTGATGATTCATAATCTGTAGGCACCATCAATAGATCGGAAGAGCACA1033140.11498537168019166No Hit
CCCTCACTGGGGTCGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA1026270.11422076136267136No Hit
AAAATCAGTTATCGTTTATTTGATAGTTCCCTGTAGGCACCATCAATAGA997840.11105658795261286No Hit
TAGTGTAACGGCTATCACATCACGCCTGTAGGCACCATCAATAGATCGGA994580.11069375976700643No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCGCTTGTCGCGTGCCAGATCGG993690.11059470544639609No Hit
AAATTCTAGAGCTAATACATGCTTCTGTAGGCACCATCAATAGATCGGAA988940.11006604474650941No Hit
GTCTAGTGGTATGATTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAG979000.10895975267137817No Hit
TCCACAGAGTTCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT976840.10871935117416655No Hit
TGGAAGAGATGTATTTATTAGATCTGTAGGCACCATCAATAGATCGGAAG972100.10819180344417438No Hit
TACCGTGGAGACCGGGGTTCGACTCCCCGTATCGGAGCCTGTAGGCACCA964010.10729141079952531No Hit
ATCCTGTCTGTGACGCCACTGTAGGCACCATCAATAGATCGGAAGAGCAC924700.1029163261442527No Hit
TTTGCCAGATCGGGGTTCAATTCCCCGTCGCGGAGCCTGTAGGCACCATC924350.10287737219794525No Hit
CCGATGAAACCCTGGTTCGATTCTAGGAGATGGCCTGTAGGCACCATCAA923160.10274492878049996No Hit
ACCTCGGTTGGATCACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC922170.10263474476094464No Hit
GCCAGATCGGGGTTCAATTCCCCGTCGCGGAGCCTGTAGGCACCATCAAT915370.10187792523268582No Hit

[FAIL]Adapter Content

Adapter graph