FastQCFastQC Report
Wed 30 Aug 2023
SRR11179727.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11179727.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67916383
Total Bases6.8 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA10047021.4793220068860262No Hit
TTGGAAGAGATGTATTTATTAGATCTGTAGGCACCATCAATAGATCGGAA6367740.9375852656935515No Hit
AAATTCTAGAGCTAATACATGCTCTGTAGGCACCATCAATAGATCGGAAG5075010.7472438572001103No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA5056250.7444816370742241No Hit
TCCACAGAATTCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT4791840.7055499407263782No Hit
ATTAAATCAGTTATCGTTTATTTGATAGTTCTGTAGGCACCATCAATAGA4430560.6523551173212507No Hit
AAAATCAGTTATCGTTTATTTGATAGTTCTGTAGGCACCATCAATAGATC4235520.6236374513642754No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3742300.5510157983531013Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATGTATTTATTAGATACTGTAGGCACCATCAATAGATCGGAAGAGCACAC3237720.47672150031900257No Hit
TTTTGATGATTCATAATCTGTAGGCACCATCAATAGATCGGAAGAGCACA3041560.4478389256977952No Hit
TCTACATGGTATAACTGTGGTAATTCTAGAGCTAATACATGCTCTGTAGG3018790.44448627365800675No Hit
TCTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCA2910300.42851221920931803No Hit
TTGGAAGAGATGTATTTATTAGATACTGTAGGCACCATCAATAGATCGGA2892140.4258383430106989No Hit
TCCACAGAATTCGCACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC2841340.41835855716874676No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCCTGTAGGCACCATCAATAGAT2375050.34970207409308No Hit
TTTGGAAGAGATGTATTTATTAGATCTGTAGGCACCATCAATAGATCGGA2190010.3224568069238905No Hit
TGATGTATTTATTAGATCTGTAGGCACCATCAATAGATCGGAAGAGCACA2173250.3199890665555614No Hit
ACCTCCCCTGGAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1937170.28522867597351287No Hit
TGATGTATTTATTAGATACTGTAGGCACCATCAATAGATCGGAAGAGCAC1889990.2782818984927392No Hit
CTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA1791440.26377140843910957No Hit
ATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAG1784090.262689195330087No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGAGCCTGTAGGCACCATCA1725340.2540388524518451No Hit
TAGAGATGTATTTATTAGATCTGTAGGCACCATCAATAGATCGGAAGAGC1703650.2508452194811376No Hit
TGGTCCTATAGTGTAGTGGTTATCACTTTCGGCTGTAGGCACCATCAATA1613500.23757154440924808No Hit
ATAACTGTGGTAATTCTAGAGCTAATACATGCTCTGTAGGCACCATCAAT1582950.23307336611256227No Hit
ATTAAATCAGTTATCGTTTATTTGATAGTTCCTGTAGGCACCATCAATAG1574810.23187483349930457No Hit
ACCTCCCCTGGAGCACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC1543050.22719849494929667No Hit
TCCTGAGTGCGAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1532250.22560830425848796No Hit
ATTTGATAGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG1517150.22338498208893132Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGGCGCTACCACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1432030.21085192360729813Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCGCTAGAGACCACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC1397900.20582662654458497No Hit
CTGTAGGCACCATCAACTGTAGGCACCATCAATAGATCGGAAGAGCACAC1385420.20398907285742823No Hit
AATTCTAGAGCTAATACATGCTCTGTAGGCACCATCAATAGATCGGAAGA1326120.1952577480458581No Hit
TCTAGAGCTAATACATGCTCTGTAGGCACCATCAATAGATCGGAAGAGCA1322750.19476154965437426No Hit
AAAATCAGTTATCGTTTATTTGATAGTTCCTGTAGGCACCATCAATAGAT1302500.1917799421091079No Hit
TACATGGTATAACTGTGGTAATTCTAGAGCTAATACATGCTCTGTAGGCA1288210.1896758842413619No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATAGGCTGTAGGCACCAT1235050.1818486122854923No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGCTGTAGGCACCATCAATA1212200.17848418105540162No Hit
TGGAAGAGATGTATTTATTAGATCTGTAGGCACCATCAATAGATCGGAAG1194970.17594723794404657No Hit
CCCCCCATCGTGAGTGCCACTGTAGGCACCATCAATAGATCGGAAGAGCA1164550.17146820083160202No Hit
TTTGGAAGAGATGTATTTATTAGATACTGTAGGCACCATCAATAGATCGG1155030.17006647718562987No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT1147030.16888855815540119No Hit
TAGAGATGTATTTATTAGATACTGTAGGCACCATCAATAGATCGGAAGAG1126200.16582155148044322No Hit
TCCACAGAATTCGCACCCTGTAGGCACCATCAATAGATCGGAAGAGCACA1121260.16509418647927704No Hit
ATGTCTAAGTATAAGCAACTGTAGGCACCATCAATAGATCGGAAGAGCAC1096020.16137785193890553No Hit
AGAGATGTATTTATTAGATCTGTAGGCACCATCAATAGATCGGAAGAGCA1078330.15877317848331235No Hit
TCCGATATAGTGTAACGGCTATCACATCACGCCTGTAGGCACCATCAATA1070720.15765268300580731No Hit
AGTTATCGTTTATTTGATAGTTCTGTAGGCACCATCAATAGATCGGAAGA1069530.15747746755006078No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT1061230.15625537655619853No Hit
TATACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGA1045110.15388186971028772No Hit
ATCCGGGTAGGACCTCCACTGTAGGCACCATCAATAGATCGGAAGAGCAC1022480.15054983125352833No Hit
AAATTCTAGAGCTAATACATGCTTCTGTAGGCACCATCAATAGATCGGAA1009310.14861068205001435No Hit
GTCTAGTCGGTTATGGCATCTGCCTGTAGGCACCATCAATAGATCGGAAG995340.1465537409434775No Hit
TTCCGATATAGTGTAACGGCTATCACATCACGCCTGTAGGCACCATCAAT989110.14563643649868693No Hit
ACCTGCGGAAGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCA985630.14512404172053744No Hit
GTCTAGTGGTATGATTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAG978690.14410219696181406No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAAT977830.14397557066606448No Hit
ATCCGGGTAGGACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT976120.1437237904733531No Hit
TATGCATGTCTAAGTATAAGCAATCTGTAGGCACCATCAATAGATCGGAA972110.14313335855945097No Hit
TATACAGTGAAACTGCGAATGGCTCATTCTGTAGGCACCATCAATAGATC939120.1382759149585454No Hit
ATTTGATAGTTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT938300.13815517825794696Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCACAGAATTCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACAC930170.13695811804347707No Hit
TCCCGCTAGAGACCACCCTGTAGGCACCATCAATAGATCGGAAGAGCACA925560.13627934220230778No Hit
GCCCCCTATGAGGAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC925010.13619836026897958No Hit
GTTTAGTGGTAAAATCCAACGTTGCCATCGTTGGGCCCCCGGTTCGATTC924560.1361321023235292No Hit
ATCCGATTGGAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT920770.13557406318295837No Hit
ACTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTCTGTAGGCAC920210.13549160885084238No Hit
TCCCGCTAGAGACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT900420.13257773164981415No Hit
ACCTCCCCTGGAGCACCCTGTAGGCACCATCAATAGATCGGAAGAGCACA898470.1322906138861959No Hit
TCCGATATAGTGTAACGGCTATCACATCACGCTGTAGGCACCATCAATAG892900.1314704877613992No Hit
TCGTTGGGCCCCCGGTTCGATTCCGGGCTTGCGCACCACTGTAGGCACCA876960.12912348409366853No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGCCTGTAGGCACCATCAATAGA869450.12801771260404135No Hit
GTCTAGTGGTATGATTCTCGCTTTGGGTGCGAGAGGCCCTGGGTTCATTT867390.12771439845375746No Hit
GCCCCCTATGAGGAGCCACTGTAGGCACCATCAATAGATCGGAAGAGCAC841940.12396714353884247No Hit
ATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAG839620.12362554702007615No Hit
CGTGGAGACCGGGGTTCGACTCCCCGTATCGGAGCCTGTAGGCACCATCA830390.1222665229389498No Hit
TTCCGATATAGTGTAACGGCTATCACATCACGCTGTAGGCACCATCAATA827880.12189695084321554No Hit
ATCCGGGTAGGACCTCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA797110.11736637977319846No Hit
TATGCATGTCTAAGTATAAGCAACTGTAGGCACCATCAATAGATCGGAAG785900.11571582073209051No Hit
CCGTGATAGTTTAATGGTCAGAATGGGCCTGTAGGCACCATCAATAGATC781390.1150517688787991No Hit
TGGTCCTATAGTGTAGTGGTTATCACTTTCGGTCTGTAGGCACCATCAAT780960.11498845573092431No Hit
TCCACAGAGTTCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT770400.11343360261102244No Hit
TATTCTAGAGCTAATACATGCTCTGTAGGCACCATCAATAGATCGGAAGA756000.1113133483566108No Hit
TCCGATATAGTGTAACGGCTATCACATCACGCTTTCACCGTGGAGACCGG751150.11059923494453466No Hit
GCCAGATCGGGGTTCAATTCCCCGTCGCGGAGCCTGTAGGCACCATCAAT736480.10843922592285282No Hit
ATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATAGATCGGAAGA735850.10834646479922229No Hit
TCTTTGATGATTCATAATCTGTAGGCACCATCAATAGATCGGAAGAGCAC732700.10788265918106976No Hit
TCGTTGGGCCCCCGGTTCGATTCCGGGCTTGCGCCTGTAGGCACCATCAA731500.10770597132653545No Hit
TATCGTTTATTTGATAGTTCTGTAGGCACCATCAATAGATCGGAAGAGCA726390.10695357554597688No Hit
TTTGCTCGAATATATTAGCATGGAATAATCTGTAGGCACCATCAATAGAT708160.10426939255584325No Hit
ATACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGAT698360.10282644174381313No Hit
TTTCTAGAGCTAATACATGCTCTGTAGGCACCATCAATAGATCGGAAGAG696750.1025893855389796No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCTCTGTAGGCACCATCAATAG691560.10182521056811875No Hit
TCTACATGGTATAACTGTGGTAATTCTAGAGCTAATACATGCTTCTGTAG689850.10157343037540736No Hit
TCTACATGGTATAACTGTGGTAATTCTCTGTAGGCACCATCAATAGATCG689180.1014747796566257No Hit
TCGTGGAGACCGGGGTTCGACTCCCCGTATCGGAGCCTGTAGGCACCATC684780.10082692418999994No Hit
CAGGGCTCATAACCCTGATGTCCTCGGCTGTAGGCACCATCAATAGATCG683800.10068262910879693No Hit
CCGTGGAGACCGGGGTTCGACTCCCCGTATCGGAGCCTGTAGGCACCATC683350.10061637116334655No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)