FastQCFastQC Report
Wed 30 Aug 2023
SRR11179728.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11179728.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85829293
Total Bases8.6 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA32941633.8380404694700214Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA13619671.5868323650295009No Hit
AAATTCTAGAGCTAATACATGCTCTGTAGGCACCATCAATAGATCGGAAG12778181.488790080095382No Hit
TTGGAAGAGATGTATTTATTAGATCTGTAGGCACCATCAATAGATCGGAA8531280.9939823225620651No Hit
ATTAAATCAGTTATCGTTTATTTGATAGTTCTGTAGGCACCATCAATAGA5082460.5921591361587937No Hit
TCCACAGAATTCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT5057300.5892277360364602No Hit
CTGTAGGCACCATCAACTGTAGGCACCATCAATAGATCGGAAGAGCACAC4252820.4954974987385717No Hit
TTGGAAGAGATGTATTTATTAGATACTGTAGGCACCATCAATAGATCGGA4044450.4712202394583397No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCCTGTAGGCACCATCAATAGAT3756570.43767924314604345No Hit
AAAATCAGTTATCGTTTATTTGATAGTTCTGTAGGCACCATCAATAGATC3737880.4355016649152639No Hit
AATTCTAGAGCTAATACATGCTCTGTAGGCACCATCAATAGATCGGAAGA3675210.42819996198733684No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA3570920.4160490987616547No Hit
TTTGGAAGAGATGTATTTATTAGATCTGTAGGCACCATCAATAGATCGGA3237640.3772185330712208No Hit
TCTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCA3031580.3532104126734447No Hit
TCCACAGAATTCGCACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC2734510.3185986863482611No Hit
TCTAGAGCTAATACATGCTCTGTAGGCACCATCAATAGATCGGAAGAGCA2475830.2884597919267493No Hit
TTTTGATGATTCATAATCTGTAGGCACCATCAATAGATCGGAAGAGCACA2316990.2699532897235912No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGAGCCTGTAGGCACCATCA2309530.269084122596699No Hit
TAGAGATGTATTTATTAGATCTGTAGGCACCATCAATAGATCGGAAGAGC2191040.2552788125611148No Hit
TGGTCCTATAGTGTAGTGGTTATCACTTTCGGCTGTAGGCACCATCAATA2150510.25055664853257037No Hit
ATTAAATCAGTTATCGTTTATTTGATAGTTCCTGTAGGCACCATCAATAG2014880.2347543512912311No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGCTGTAGGCACCATCAATA2012360.23446074523764282No Hit
TTTGGAAGAGATGTATTTATTAGATACTGTAGGCACCATCAATAGATCGG1933820.22531002323414223No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGCCTGTAGGCACCATCAATAGA1888120.21998550075438697No Hit
TGATGTATTTATTAGATACTGTAGGCACCATCAATAGATCGGAAGAGCAC1874150.21835785132239177No Hit
GCCCCCTATGAGGAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1782960.20773327353401363No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT1657930.19316598588316464No Hit
ACCTCCCCTGGAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1632730.1902299253472821No Hit
ATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAG1612520.18787525140163977No Hit
CTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA1600960.18652839188597303No Hit
TCCCGCTAGAGACCACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC1598540.1862464368662573No Hit
AAATTCTAGAGCTAATACATGCTTCTGTAGGCACCATCAATAGATCGGAA1595310.18587010847217394No Hit
TGGAAGAGATGTATTTATTAGATCTGTAGGCACCATCAATAGATCGGAAG1566950.18256587526591883No Hit
TAGAGATGTATTTATTAGATACTGTAGGCACCATCAATAGATCGGAAGAG1517300.17678113694819786No Hit
ATGTATTTATTAGATACTGTAGGCACCATCAATAGATCGGAAGAGCACAC1508930.17580594541306546No Hit
TCCTGAGTGCGAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1479290.1723525789732417No Hit
AAAATCAGTTATCGTTTATTTGATAGTTCCTGTAGGCACCATCAATAGAT1469620.17122592399776612No Hit
ATGTCTAAGTATAAGCAACTGTAGGCACCATCAATAGATCGGAAGAGCAC1467460.17097426166611904No Hit
TCTACATGGTATAACTGTGGTAATTCTAGAGCTAATACATGCTCTGTAGG1445300.16839239255996202No Hit
GTCTAGTGGTATGATTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAG1405550.16376110659562348No Hit
ACCTCCCCTGGAGCACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC1364500.15897835719094178No Hit
CCCCCCATCGTGAGTGCCACTGTAGGCACCATCAATAGATCGGAAGAGCA1324340.1542993019877258No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAAT1294880.15086690740887262No Hit
ATGTACACGGAGTCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1284240.14962723740483333No Hit
TTCCGATATAGTGTAACGGCTATCACATCACGCCTGTAGGCACCATCAAT1282920.14947344375771568No Hit
TCCACAGAATTCGCACCCTGTAGGCACCATCAATAGATCGGAAGAGCACA1257940.14656301549635273No Hit
CCGTGATAGTTTAATGGTCAGAATGGGCCTGTAGGCACCATCAATAGATC1224950.14271933942179857No Hit
TCCCGCTAGAGACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1207400.1406745829771661No Hit
AGAGATGTATTTATTAGATCTGTAGGCACCATCAATAGATCGGAAGAGCA1204420.1403273821677641No Hit
TTCCGATATAGTGTAACGGCTATCACATCACGCTTTCACCGTGGAGACCG1194880.13921587353632286No Hit
TCGTGGAGACCGGGGTTCGACTCCCCGTATCGGAGCCTGTAGGCACCATC1157330.1348409103171804No Hit
TAATTCTAGAGCTAATACATGCTCTGTAGGCACCATCAATAGATCGGAAG1155500.13462769639731276No Hit
GTTTAGTGGTAAAATCCAACGTTGCCATCGTTGGGCCCCCGGTTCGATTC1149900.13397523850044996No Hit
GTCTAGTGGTATGATTCTCGCTTTGGGTGCGAGAGGCCCTGGGTTCATTT1141600.13300820268902833No Hit
ACTGTAGGCACCATCAACTGTAGGCACCATCAATAGATCGGAAGAGCACA1123200.13086441245647917No Hit
TCCACAGAATTCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1122760.13081314790743995No Hit
TCCCGCTAGAGACCACCCTGTAGGCACCATCAATAGATCGGAAGAGCACA1101790.12836992610436626No Hit
TGATGTATTTATTAGATCTGTAGGCACCATCAATAGATCGGAAGAGCACA1097920.1279190310934986No Hit
CGTGGAGACCGGGGTTCGACTCCCCGTATCGGAGCCTGTAGGCACCATCA1097280.12784446447671427No Hit
TCCGATATAGTGTAACGGCTATCACATCACGCTTTCACCGTGGAGACCGG1075000.12524861412991017No Hit
GTCTAGTCGGTTATGGCATCTGCCTGTAGGCACCATCAATAGATCGGAAG1062420.12378291406874341No Hit
CAGGGCTCATAACCCTGATGTCCTCGGCTGTAGGCACCATCAATAGATCG1056010.12303608279751296No Hit
TATTCTAGAGCTAATACATGCTCTGTAGGCACCATCAATAGATCGGAAGA1052890.1226725705406894No Hit
GCCAGATCGGGGTTCAATTCCCCGTCGCGGAGCCTGTAGGCACCATCAAT1047050.12199215016253251No Hit
TCCGATATAGTGTAACGGCTATCACATCACGCCTGTAGGCACCATCAATA1034630.12054509175556181No Hit
TATGCATGTCTAAGTATAAGCAATCTGTAGGCACCATCAATAGATCGGAA1026630.11961300904575783No Hit
AAATGGTCAGAATGGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA1019030.11872753047144405No Hit
TCGTTGGGCCCCCGGTTCGATTCCGGGCTTGCGCACCACTGTAGGCACCA1015530.1183197442859048No Hit
TTTCTAGAGCTAATACATGCTCTGTAGGCACCATCAATAGATCGGAAGAG1014520.11820206884379206No Hit
ACCTCCCCTGGAGCACCCTGTAGGCACCATCAATAGATCGGAAGAGCACA1007710.11740863343707142No Hit
GCCCCCTATGAGGAGCCACTGTAGGCACCATCAATAGATCGGAAGAGCAC1004180.1169973519413704No Hit
AGAGCTAATACATGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1002870.11684472339764No Hit
TTCCGATATAGTGTAACGGCTATCACATCACGCTGTAGGCACCATCAATA999820.11648936686452724No Hit
TGGTCCTATAGTGTAGTGGTTATCACTTTCGGTCTGTAGGCACCATCAAT997440.11621207225836055No Hit
GCGTAGTCGGTAGCGCTCTCCCCTGTAGGCACCATCAATAGATCGGAAGA982630.11448655414183592No Hit
CCGTGGAGACCGGGGTTCGACTCCCCGTATCGGAGCCTGTAGGCACCATC973230.11339135695781624No Hit
ATGTCTAAGTATAAGCAATCTGTAGGCACCATCAATAGATCGGAAGAGCA957920.1116075836719289No Hit
ATCCGATTGGAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT951090.11081181805843374No Hit
TATGCATGTCTAAGTATAAGCAACTGTAGGCACCATCAATAGATCGGAAG919670.10715106321567858No Hit
AGTTATCGTTTATTTGATAGTTCTGTAGGCACCATCAATAGATCGGAAGA887560.10340991623920286No Hit
ATAACTGTGGTAATTCTAGAGCTAATACATGCTCTGTAGGCACCATCAAT878620.10236831381099691No Hit
ATGTATTTATTAGATAAAAAATCAATGTCTGTAGGCACCATCAATAGATC859690.10016277309892324No Hit
ACCTGCGGAAGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCA859370.10012548979053107No Hit
ATCCGGGTAGGACCTCCACTGTAGGCACCATCAATAGATCGGAAGAGCAC858830.1000625742076193No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)