FastQCFastQC Report
Wed 30 Aug 2023
SRR11179729.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11179729.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93325194
Total Bases9.4 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTACACGGAGTCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG8892890.9528927419106141No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA6961390.7459282645584429Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA6910960.7405245790327529No Hit
ATGTATTTATTAGATACTGTAGGCACCATCAATAGATCGGAAGAGCACAC6673150.7150427139749638No Hit
TTGGAAGAGATGTATTTATTAGATCTGTAGGCACCATCAATAGATCGGAA6568900.7038720969602271No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCCTGTAGGCACCATCAATAGAT6553440.702215523923797No Hit
TTGGAAGAGATGTATTTATTAGATACTGTAGGCACCATCAATAGATCGGA5158690.5527649907698021No Hit
TTTGGAAGAGATGTATTTATTAGATCTGTAGGCACCATCAATAGATCGGA4990330.5347248461117584No Hit
AAATTCTAGAGCTAATACATGCTCTGTAGGCACCATCAATAGATCGGAAG4931730.5284457270991583No Hit
TTTGGAAGAGATGTATTTATTAGATACTGTAGGCACCATCAATAGATCGG4642860.49749267062868363No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGAGCCTGTAGGCACCATCA4403180.4718104309539394No Hit
TCCACAGAATTCGCACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC4231550.4534198985967283No Hit
TCTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCA3974480.4258742821365043No Hit
TACACGGAGTCGACCCGCAACGCCTGTAGGCACCATCAATAGATCGGAAG3609930.38681194705043953No Hit
TCCGATATAGTGTAACGGCTATCACATCACGCCTGTAGGCACCATCAATA3603940.38617010536297414No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGCTGTAGGCACCATCAATA3572680.3828205275415768No Hit
CTGTAGGCACCATCAACTGTAGGCACCATCAATAGATCGGAAGAGCACAC3526880.37791295670920333No Hit
ACGGAGTCGACCCGCAACGCCTGTAGGCACCATCAATAGATCGGAAGAGC3359970.36002818274345083No Hit
TGGTCCTATAGTGTAGTGGTTATCACTTTCGGCTGTAGGCACCATCAATA3000530.32151339540746093No Hit
TCCACAGAATTCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2882430.3088587204008384No Hit
TGATGTATTTATTAGATCTGTAGGCACCATCAATAGATCGGAAGAGCACA2758330.2955611321847346No Hit
TCCGATATAGTGTAACGGCTATCACATCACGCTGTAGGCACCATCAATAG2741890.29379954999075597No Hit
ATTAAATCAGTTATCGTTTATTTGATAGTTCTGTAGGCACCATCAATAGA2719500.2914004121973751No Hit
CTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA2618390.2805662530956003No Hit
TCGTTGGGCCCCCGGTTCGATTCCGGGCTTGCGCACCACTGTAGGCACCA2605800.2792172068777055No Hit
TGATGTATTTATTAGATACTGTAGGCACCATCAATAGATCGGAAGAGCAC2512150.26918240319971903No Hit
AAAATCAGTTATCGTTTATTTGATAGTTCTGTAGGCACCATCAATAGATC2409010.25813072512873636No Hit
ATGTACACGGAGTCGACCCGCAACGCCTGTAGGCACCATCAATAGATCGG2274330.243699466619914No Hit
ACACGGAGTCGACCCGCAACGCCTGTAGGCACCATCAATAGATCGGAAGA2269450.24317656387620262No Hit
GTCTAGTCGGTTATGGCATCTGCCTGTAGGCACCATCAATAGATCGGAAG2249480.24103673441064588No Hit
TCCCGCTAGAGACCACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC2211870.23700674010921427No Hit
TTTTGATGATTCATAATCTGTAGGCACCATCAATAGATCGGAAGAGCACA2211430.23695959314051895No Hit
TTCCGATATAGTGTAACGGCTATCACATCACGCCTGTAGGCACCATCAAT2063830.2211439281872803No Hit
TGGAAGAGATGTATTTATTAGATCTGTAGGCACCATCAATAGATCGGAAG2051510.21982381306381213No Hit
GTCTAGTGGTATGATTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAG2018590.21629636258779167No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGCCTGTAGGCACCATCAATAGA2008040.21516590686112047No Hit
TGTACACGGAGTCGACCCGCAACGCCTGTAGGCACCATCAATAGATCGGA1939260.20779597843643377No Hit
CGTGGAGACCGGGGTTCGACTCCCCGTATCGGAGCCACTGTAGGCACCAT1912950.20497680401285853No Hit
TGGTCCTATAGTGTAGTGGTTATCACTTTCGGTCTGTAGGCACCATCAAT1899520.2035377499456363No Hit
CGTTGGGCCCCCGGTTCGATTCCGGGCTTGCGCACCACTGTAGGCACCAT1892100.2027426806099112No Hit
CGTGGAGACCGGGGTTCGACTCCCCGTATCGGAGCCTGTAGGCACCATCA1872000.200588921358149No Hit
TCGACCCGCAACGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1852960.19854874344006185No Hit
ATTAAATCAGTTATCGTTTATTTGATAGTTCCTGTAGGCACCATCAATAG1767350.18937544346278026No Hit
CCCCCCATCGTGAGTGCCACTGTAGGCACCATCAATAGATCGGAAGAGCA1723390.18466503268131432No Hit
GCCAGATCGGGGTTCAATTCCCCGTCGCGGAGCCTGTAGGCACCATCAAT1672680.179231344539182No Hit
AACGCGATGTACACGGAGTCGACCCGCAACGCCTGTAGGCACCATCAATA1671300.17908347450100132No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA1651470.1769586463436658No Hit
TAGAGATGTATTTATTAGATACTGTAGGCACCATCAATAGATCGGAAGAG1645180.17628465899572626No Hit
AAATTCTAGAGCTAATACATGCTTCTGTAGGCACCATCAATAGATCGGAA1634520.17514241652688126No Hit
CCGTGGAGACCGGGGTTCGACTCCCCGTATCGGAGCCTGTAGGCACCATC1615900.17314724253345778No Hit
TCCACAGAATTCGCACCCTGTAGGCACCATCAATAGATCGGAAGAGCACA1567300.1679396455366597No Hit
TTCCGATATAGTGTAACGGCTATCACATCACGCTGTAGGCACCATCAATA1556200.16675025609911936No Hit
ATCCGATTGGAAGCACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC1490970.15976071799004243No Hit
CCGTGATAGTTTAATGGTCAGAATGGGCCTGTAGGCACCATCAATAGATC1488780.15952605466858177No Hit
ATCCGGGTAGGACCTCCACTGTAGGCACCATCAATAGATCGGAAGAGCAC1485410.15916495174925646No Hit
TGGAAGAGATGTATTTATTAGATACTGTAGGCACCATCAATAGATCGGAA1473160.15785233727989892No Hit
AGAGATGTATTTATTAGATCTGTAGGCACCATCAATAGATCGGAAGAGCA1464990.15697690379298865No Hit
GCGTAGTCGGTAGCGCTCTCCCCTGTAGGCACCATCAATAGATCGGAAGA1459710.15641114016864516No Hit
CCGTGGAGACCGGGGTTCGACTCCCCGTATCGGAGCCACTGTAGGCACCA1425700.15276689379290226No Hit
TATCGTTGGGCCCCCGGTTCGATTCCGGGCTTGCGCACCACTGTAGGCAC1424340.15262116679875318No Hit
TGGGAGAGGTCTCCGGTTCGATTCCGGACTCGTCCACCCTGTAGGCACCA1407340.15079957937188967No Hit
TAGAGATGTATTTATTAGATCTGTAGGCACCATCAATAGATCGGAAGAGC1406490.15070850000054647No Hit
TCCCGCTAGAGACCACCCTGTAGGCACCATCAATAGATCGGAAGAGCACA1397740.14977091823671965No Hit
TCCACAGAATTCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1386870.1486061738055428No Hit
AGAGATGTATTTATTAGATACTGTAGGCACCATCAATAGATCGGAAGAGC1380240.14789575470906602No Hit
ATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAG1328930.14239777524598557No Hit
TCTTTGATGATTCATAATCTGTAGGCACCATCAATAGATCGGAAGAGCAC1318190.14124696060101413No Hit
GTTTAGTGGTAAAATCCAACGCTGTAGGCACCATCAATAGATCGGAAGAG1315270.14093407617239992No Hit
GCCCCCTATGAGGAGCCACTGTAGGCACCATCAATAGATCGGAAGAGCAC1300860.13939001294762912No Hit
GTTTAGTGGTAAAATCCAACGTTGCCTGTAGGCACCATCAATAGATCGGA1295220.13878567453071675No Hit
GCCAGATCGGGGTTCAATTCCCCGTCGCGGCTGTAGGCACCATCAATAGA1282440.1374162693945217No Hit
ACCTCCCCTGGAGCACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC1270350.136120799277417No Hit
TCGTTGGGCCCCCGGTTCGATTCCGGGCTTGCGCCTGTAGGCACCATCAA1237610.132612636197681No Hit
TAATCAGAAGATTATGGGTTCCCCCCCATCGTGAGTGCCACTGTAGGCAC1228180.13160219093677963No Hit
AAAATCAGTTATCGTTTATTTGATAGTTCCTGTAGGCACCATCAATAGAT1216420.13034208104619638No Hit
AAATGGTCAGAATGGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA1205820.12920626770944618No Hit
TCGTGGAGACCGGGGTTCGACTCCCCGTATCGGAGCCTGTAGGCACCATC1186680.12715537457120102No Hit
ATGTATTTATTAGATAAAAAATCAATGTCTGTAGGCACCATCAATAGATC1164060.1247315917714567No Hit
TCCTTGTTAGCTCAGTTGGTAGAGCTTTCGGCTGTAGGCACCATCAATAG1131100.12119985520737304No Hit
GCCCCCTATGAGGAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1130130.12109591757184024No Hit
TCCGATGTAGTGTAACGGCTATCACATCACGCTGTAGGCACCATCAATAG1107550.11867642086015916No Hit
TGATGTACACGGAGTCGACCCGCAACGCCTGTAGGCACCATCAATAGATC1105030.11840639731217704No Hit
TCGTGGAGACCGGGGTTCGACTCCCCGTATCGGAGCCACTGTAGGCACCA1096790.11752346317115611No Hit
TCGACCCGCAACGCGATGTACACGGAGTCGACCCGCAACGCCTGTAGGCA1091800.11698877368527087No Hit
ACGCGATGTACACGGAGTCGACCCGCAACGCCTGTAGGCACCATCAATAG1091210.11692555388633857No Hit
TCACCGTGGAGACCGGGGTTCGACTCCCCGTATCGGAGCCTGTAGGCACC1083120.11605869257555468No Hit
CCGATGAAACCCTGGTTCGATTCTAGGAGATGGCCTGTAGGCACCATCAA1082820.11602654691508063No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGAGCCCTGTAGGCACCATC1055470.11309593420186194No Hit
CCGATATAGTGTAACGGCTATCACATCACGCCTGTAGGCACCATCAATAG1047920.11228693507993137No Hit
ACGGAGTCGACCCGCAACGCGATGTACACGGAGTCGACCCGCAACGCCTG1046590.11214442265182968No Hit
TTGGGAGAGGTCTCCGGTTCGATTCCGGACTCGTCCACCCTGTAGGCACC1028600.11021675454540175No Hit
TCCTGGGCGAAATCACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC1010090.1082333672941521No Hit
GCGATGTACACGGAGTCGACCCGCAACGCCTGTAGGCACCATCAATAGAT1005980.10779297174565744No Hit
CCCCCCATCGTGAGTGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA995770.10669894776752353No Hit
TCCTGGGCGAAATCACCCTGTAGGCACCATCAATAGATCGGAAGAGCACA992220.10631855745191379No Hit
GTTTAGTGGTAAAATCCAACGTTGCCATCGTTGGGCCCCCGGTTCGATTC982450.10527168044247516No Hit
CTTTGATGATTCATAATCTGTAGGCACCATCAATAGATCGGAAGAGCACA960140.10288111482522073No Hit
GCAACGCGATGTACACGGAGTCGACCCGCAACGCCTGTAGGCACCATCAA952520.10206461504917953No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGACTGTAGGCACCATCAAT938490.10056126966100923No Hit
TATCGTTGGGCCCCCGGTTCGATTCCGGGCTTGCGCCTGTAGGCACCATC937540.10045947506950803No Hit
TCACCGTGGAGACCGGGGTTCGACTCCCCGTATCGGAGCCACTGTAGGCA933940.10007372714381926No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)