FastQCFastQC Report
Mon 3 Jul 2023
SRR11218270_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11218270_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128552577
Total Bases6.5 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG46199423.5938151593802745TruSeq Adapter, Index 1 (100% over 49bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCT15876491.2350191937420283Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTG15117411.1759709803405964Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTG12353740.9609873476126426Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTC10507830.8173955159218628No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTGA10329390.8035148140204144Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT10135450.788428379774915No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACG9024520.7020100421635266No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACAC7768970.6043418328362254No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGT7369760.5732876128963171No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGT7349030.5716750431226283No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCAGATCGGAAGAGCA6776000.5270995073089823No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA6309260.49079218380818607No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCAGATCGGAAGAGC5956170.4633256010107055No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACGT5666380.440783073527962No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCT5144210.40016389558647275Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCAGATCGGAAGAGCAC5051230.39293105730583683No Hit
GCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCAGATCGGAAGAGCA5037510.391863789708393No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC4979700.3873667970110004No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC4485750.34894282982751873No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACAC4313220.33552186200047934No Hit
CGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCGAGATCGGAAGAGCA4048460.3149263977804195No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACG3916010.30462322042754536No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACACG3834790.2983051829447184No Hit
GTGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGC3668630.2853797322164922No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTC3618950.2815151655808502No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAAGATCGGAAGAGCACACGTCTGA3569660.27768093672676825Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCAGATCGGAAGAGCAC3251690.2529463100533566No Hit
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCAC2924140.22746646300213802No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA2886180.2245135855969655Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCAGATCGGAAGAGCACA2849650.2216719467241796No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGTC2482280.19309453438650243No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC2186530.17008838337017546No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCAGATCGGAAGAG2180600.1696270935120966No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAAGATCGGAAGAGCACACGTCTGAA2107710.16395703992771768Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCGAGATCGGAAGAGC1993680.15508673933467707No Hit
TGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCA1955480.15211519252546762No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGA1946890.15144698343931293Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACAC1758980.13682961797024107No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCAGATCGGAAGAGCAC1680250.13070527555429712No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGT1665880.12958744498758668No Hit
GCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCAGATCGGAAGAGCACA1556170.12105319366721057No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC1492540.1161034679219227No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCGAGATCGGAAGAG1430910.11130932054360916No Hit
CGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCAGATCGGAAGAGCACAC1415150.11008336301185156No Hit
ATTTAAGGAGAGGGTATCAAGCACATTAAAGATCGGAAGAGCACACGTCT1407350.10947660738065174Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAAGATCGGAAGAGCACACGT1395680.1085688076093566No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACGTC1360800.10585552088932453No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT1337930.10407648226297324No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCAGATCGGAAGAGC1296840.10088012471348591No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)