FastQCFastQC Report
Mon 3 Jul 2023
SRR11218275_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11218275_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109137992
Total Bases5.5 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG70653026.473732813409285TruSeq Adapter, Index 4 (100% over 49bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT27082042.481449356334135No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT13149241.204827004696953No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGT11595011.0624173844063394No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACA11230891.0290541171034189No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA11176241.0240466949401086No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCGAGATCGGAAGAG9677300.8867031381702534No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACG8158140.7475068810135338No Hit
TGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCGGAAGAGCACACG7582140.6947296593105726No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG7503140.6874911167506179No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCAGATCGGAAGAG6622700.6068189343267374No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACAC5727090.5247567684771037No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACA5439180.49837640406651423No Hit
GCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCGAGATCGGAAGAGC5352480.4904323326747665No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTC5337920.4890982417928305No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGT4761500.4362825366990443No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACG4750330.43525906175733925No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCT4126420.3780919846866891Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCAGATCGGAAGAGC4112770.3768412744848742No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT4018490.3682026695158548No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACG4018470.36820083697343453No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACA3798890.3480813537416008No Hit
GCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCAGATCGGAAGAGCA3753520.34392423126128246No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA3633430.33292073029894115Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACAC3614730.3312073031360152No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC3551490.3254128040032109No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA3275740.3001466253841284Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTC3246850.29749951785808926No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTG3097280.2837948493683116Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCGAGATCGGAAGA2869700.2629423491683813No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACAC2866370.262637230855411No Hit
CGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCGAGATCGGAAGAGCA2767230.25355331807827286No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACAC2758100.25271676246343255No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCT2628220.24081623198638288Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCAGATCGGAAGAGCA2592660.23755797156319314No Hit
ACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAGAGCACACGTCTGA2566300.23514268065331456Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAAGATTAAGCCATGCATGTCTAAGTACGCAGATCGGAAGAGCACACGTC2542410.23295370873233587No Hit
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC2334380.21389251874819173Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGT2284660.20933681829147086No Hit
AGATCGGAAGAGCACACGTCTACAGAGTTCTACAGTCCGACGATCAGATC2258410.2069316063649036Illumina DpnII expression Sequencing Primer (100% over 23bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCAGATCGGAAGAGCACACGT2242820.20550313954832522No Hit
CAGGAAGAAACCATGGTGCTCTCTGGGGAAGATCGGAAGAGCACACGTCT2221540.20355331441318805Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCAGATCGGAAGAGCAC2167340.19858712445433302No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTGA2074270.1900593883017382Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACACG2072050.1898559760930914No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC1952620.17891294903061805No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTG1914350.17540637910948553Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCT1889400.173120282440234Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC1837220.1683391792658234TruSeq Adapter, Index 4 (100% over 50bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC1713210.1569764999891147No Hit
GTGAAGGCCCATGGCAAGAAAGTGATAACAGATCGGAAGAGCACACGTCT1535560.14069894194131774Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCAGATCGGAAGAGCAC1522490.1395013754696898No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCAGATCGGAAGAGC1512980.13863000154886485No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA1489790.1365051686125946Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGAGATCGGAAGAGCA1451920.13303524953986692No Hit
GTGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGC1429590.13098921592766707No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG1400850.12835585246977974Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCAC1334280.12225623502400519No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCACAC1305690.1196366156342697No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGATCGGAAGAGCACACG1262360.11566641248081602No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGTC1257350.11520736060454549No Hit
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACAC1209370.11081109133838563No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG1181630.10826935500151039No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAAGATCGGAAGAGCACAC1179030.10803112448687896No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCTCAGATCGGAA1178820.10801188279146642No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC1147720.10516227932799056No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAGATCGGAAGAGCACACG1147410.10513387492047682No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACAC1125530.10312907351273239No Hit
AGTGAAACTGCGAATGGCTCATTAAATCAGATCGGAAGAGCACACGTCTG1109490.10165937449169854Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAGGAAGAAACCATGGTGCTCTCTGGGGAAAGATCGGAAGAGCACACGTC1108650.1015824077100484No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)