FastQCFastQC Report
Tue 4 Jul 2023
SRR11218276_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11218276_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences120416195
Total Bases6.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT11446500.9505781178353958No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA10857360.9016528050898802Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT9816330.8152001481196113No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA9147400.7596486502500764No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG9083990.7543827472708302Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA6634780.5509873485040778Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA6216500.5162511570806567Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC4156630.3451886185242774No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT3676340.3053027875527872No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC3657510.3037390444034542No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACAC3548300.29466966631855457No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT3423110.28427322421207546Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG3410120.2831944656613672No Hit
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA3212020.2667431901497967Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACG3178000.2639179887721913No Hit
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACG3164060.2627603371788986No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGT2895060.2404211493312839No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACAC2891840.24015374343957638No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACG2804880.232932123457314No Hit
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC2679770.22254232497547363Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGT2480600.20600219098436054No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACGT2430460.20183829924205793No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG2205230.1831340045248897No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACG2093230.17383293003071554No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC2093040.17381715142219867No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG2002850.16632729509514896TruSeq Adapter, Index 5 (100% over 49bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAGATCGGAAGAGCACACGT1943390.16138942108243828No Hit
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC1779700.1477957346185868Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA1748260.14518479013557936No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT1553840.12903912135738885No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACACG1548760.12861725119283168No Hit
AGGGCTTCTGTCGTGAGTGGCACACGCAGAGATCGGAAGAGCACACGTCT1523360.12650790037004575Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT1440150.11959770029272225Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACAC1410200.11711049331861051No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTC1396450.11596862033383466No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTC1385370.1150484783213753No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACAC1295690.10760097510139728No Hit
GACCTGGTATTGCAGTACCTCCAGGAGATCGGAAGAGCACACGTCTGAAC1229740.10212413703987243Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGC1217030.1010686311753996No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)