FastQCFastQC Report
Mon 3 Jul 2023
SRR11218278_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11218278_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105326761
Total Bases5.3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG19417881.8435846517676546TruSeq Adapter, Index 3 (100% over 49bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGT14641511.3901035084521398No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA10348890.982550863782852Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCTGGGGCGATCCACGGGAAGGGCCCGGCTCGCGTCCAGATCGGAAGAG9636190.914885249343232No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC6621070.6286218181531282No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACAC6137220.582683825243615No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACG6005550.5701827287748837No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA5914240.5615135169683989Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT5600010.5316796934446697No Hit
GCGTCGATGAAGAACGCAGCTAGCTGCGAGAATTAGATCGGAAGAGCACA5590460.5307729913008528No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA5577870.5295776635531402Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCAC5522190.524291257755472No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTC5169720.4908268279511605No Hit
ACGGACCAAGGAGTCTAACGCGTGCGCGAGTCAGATCGGAAGAGCACACG5013230.4759692553348337No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA4966980.4715781585650393No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTC4964030.47129807779810107No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACA4822820.4578912286118815No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCT4740230.4500499165639395Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGATCGGAAGAGCACACG4514650.42863275744328644No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT4304640.40869385511627004No Hit
AAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAATAGATCGGAAGA4289980.4073019961185363No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG4188430.3976605717515609No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACA4035940.3831827696666757No Hit
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACAGATCGGAAGAGCA3780460.3589268258234961No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA3256320.30916359423603657No Hit
GCGAGTGCCTAGTGGGCCACTTTTGGTAAGCAGATCGGAAGAGCACACGT3195610.3033996269950806No Hit
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACAGATCGGAAGAGC3137400.2978730163362756No Hit
TGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCGGAAGAGCACACG3048430.28942597029068423No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTAGATCGGAAGAGCACACGT2972150.28218374625609155No Hit
GAACGGAAGGACGGACGGCGCCGGACGCGCAGATCGGAAGAGCACACGTC2627550.24946651497239147No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG2567060.2437234351106648No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG2340010.22216671031970686Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAGATCGGAAGAGCAC2241380.21280251844068382No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGCAGATCGGAAGAGCACAC2226490.21138882263739223No Hit
ACGCGCGCGCGCGCGCGCCGCCCCCGCCGCTCCCGTCCAGATCGGAAGAG2195770.20847218495592018No Hit
AAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGTAGATCGGAAGAGCACA2171880.2062040054568848No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACA2157700.20485771892292406No Hit
AGATCGGAAGAGCACACGTCTACAGAGTTCTACAGTCCGACGATCAGATC2156640.204757079732092Illumina DpnII expression Sequencing Primer (100% over 23bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACG2117140.20100684573410552No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACAC2067950.19633661762370153No Hit
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA2013940.19120876602291037Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCT2006570.19050903881872908Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTG1922850.18256044159565488Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT1892060.1796371579298826Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCAGATCGGAAGAG1784410.1694165835024586No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACG1777750.16878426556760823No Hit
AATGATAGGAAGAGCCGACATCGAAGGATCAGATCGGAAGAGCACACGTC1753680.16649899639465796No Hit
ATGCGAGTGCCTAGTGGGCCACTTTTGGTAAGCAGATCGGAAGAGCACAC1698600.16126955617670613No Hit
TACAGTGAAACTGCGAATGGCTCATTAAATCAGATCGGAAGAGCACACGT1679890.1594931795158877No Hit
AGGGCGAAGCCAGAGGAAACTCTGGTGGAGGTCCAGATCGGAAGAGCACA1675240.15905169627308677No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACAAGATCGGAAGAGCACACG1650740.15672560176800654No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACAC1633590.15509733561445033No Hit
AGGGCGAAGCCAGAGGAAACTCTGGTGGAGGTCAGATCGGAAGAGCACAC1623810.15416879666507546No Hit
AAACGATGCCGACTGGCGATGCGGCGGCGTTAGATCGGAAGAGCACACGT1547570.14693037033579717No Hit
AACCCCCGGGACGGGGACCGGCGGGCCACGGGCCCGGCTCAGATCGGAAG1513860.1437298541820725No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACACG1489430.14141040566129248No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT1444680.13716172284078879No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTAGATCGGAAGAGCACACGT1443540.1370534882393279No Hit
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACCAGATCGGAAGAGC1420540.1348698076835383No Hit
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC1398290.132757334102394Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGCGAGTGCCTAGTGGGCCACTTTTGGTAAGCAGATCGGAAGAGCACACG1395780.13251902809391433No Hit
AGACGTGGCGACCCGCTGAATTTAAGCATAGATCGGAAGAGCACACGTCT1342250.12743674895689616Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC1339300.1271566681899579No Hit
CGGACCAAGGAGTCTAACGCGTGCGCGAGTCAGATCGGAAGAGCACACGT1313980.12475272072593213No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTG1298820.1233133904117682Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACAC1284460.12195001420389259No Hit
GAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGAGATCGGAAGAGCAC1283800.12188735206620473No Hit
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC1267430.12033314116627968Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCCGCGGACGCTACGCCGCGACGAGTAGGAGGGCAGATCGGAAGAGCACA1263600.11996951088242427No Hit
ATTGTTAGGACCTAAACTCAATAACGAAAGTAGATCGGAAGAGCACACGT1255350.11918623416132582No Hit
CTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAA1209850.11486634436617679Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAAGATCGGAAGAGCACACGTC1177480.11179305134048506No Hit
ATGATTAAGAGGGACGGCCGGGGGCATTCGTAGATCGGAAGAGCACACGT1162030.1103261876627916No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCAGATCGGAAGAGC1143250.10854316501767294No Hit
CCATGACCCGCCGGGCAGCTTCCGGGAAACCAGATCGGAAGAGCACACGT1139610.10819757383406102No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACA1073990.1019674382657604No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)