FastQCFastQC Report
Mon 3 Jul 2023
SRR11218279_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11218279_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117231120
Total Bases5.9 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG68640315.855127034528033TruSeq Adapter, Index 4 (100% over 49bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGT15549591.3264046270307748No Hit
AAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAATAGATCGGAAGA14067491.19997915229335No Hit
GCGTCGATGAAGAACGCAGCTAGCTGCGAGAATTAGATCGGAAGAGCACA11116300.9482379764008055No Hit
ATCGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAGAGCACAC9144190.7800138734493025No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA6925210.5907313689402609No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT6596030.5626517941652354No Hit
AGATCGGAAGAGCACACGTCTACAGAGTTCTACAGTCCGACGATCAGATC6331290.5400690533366909Illumina DpnII expression Sequencing Primer (100% over 23bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA5866350.5004089357842866Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCAC5817280.4962231871537182No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA5619120.4793198256572146Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACAC5413240.4617579359473833No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGATCGGAAGAGCACACG5262910.4489345491197218No Hit
ATCGACACTTCGAACGCACTTGCGGCCCCGGGAGATCGGAAGAGCACACG4665970.39801462273839916No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACG4573360.3901148432259284No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC4184290.35692655670269124No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACA3966200.33832313467618497No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTC3940370.3361197948121625No Hit
AAACGATGCCGACTGGCGATGCGGCGGCGTTAGATCGGAAGAGCACACGT3861400.32938352887868No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA3565590.3041504678962378Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTC3508330.29926609930878423No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACAC3407790.290689878250758No Hit
ACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAGATCGGAAGAGCA3403640.29033587668530336No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT3302220.28168459023508435Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT3275340.2793916837099228No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACA3245750.2768676098974402No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG2990390.2550849979084052No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACA2828900.2413096454252079No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA2792380.23819443164920712No Hit
AGCTGGGGCGATCCACGGGAAGGGCCCGGCTCGCGTCCAGATCGGAAGAG2597440.22156574124686346No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCTCAGATCGGA2479560.21151039075631112No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT2427850.20709944594916435No Hit
ACGGACCAAGGAGTCTAACGCGTGCGCGAGTCAGATCGGAAGAGCACACG2411200.20567917460824395No Hit
TGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCGGAAGAGCACACG2350480.2004996625469415No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG2293720.19565794475050652Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCT2244440.1914542827877103Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCTCAGATCGGAA2239760.19105507138377592No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCAGATCGGAAGAG2184580.18634813008695986No Hit
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC2085070.177859769658432Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAGAGCACACGTCTGA2068320.17643096815930787Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAA1969210.16797672836359492Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCGAGATCGGAAGA1914920.16334570547479202No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC1858380.15852275402640528No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGAA1808400.15425938095618297No Hit
AGCGCCGAATCCCCGCCGCGCGTCGCGGCGTGGGAAATAGATCGGAAGAG1745100.14885979081322434No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACG1703810.14533768849090584No Hit
GCGAGTGCCTAGTGGGCCACTTTTGGTAAGCAGATCGGAAGAGCACACGT1676150.1429782467317552No Hit
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA1628330.13889912507873337Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCATGACCCGCCGGGCAGCTTCCGGGAAACCAGATCGGAAGAGCACACGT1462240.12473138531816468No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACG1461860.12469897071699051No Hit
TACAGTGAAACTGCGAATGGCTCATTAAATCAGATCGGAAGAGCACACGT1458630.12442344660701014No Hit
AGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAATAGATCGGAAGAG1449140.12361393459347654No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG1445840.1233324393727536No Hit
GTGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGC1428010.12181151216502921No Hit
GAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGAGATCGGAAGAGCAC1408520.12014898433112299No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTAGATCGGAAGAGCACACGT1344000.11464532625807891No Hit
AGACGTGGCGACCCGCTGAATTTAAGCATAGATCGGAAGAGCACACGTCT1331130.11354749489725936Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGTAGATCGGAAGAGCA1324560.11298706350327456No Hit
AGATCGGAAGAGCACACTTCTGAACTCCAGTCACTGACCAATCTCGTATG1273040.10859232599671487TruSeq Adapter, Index 4 (97% over 49bp)
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCAGATCGGAAGAGC1259340.1074236943228044No Hit
ACGCGCGCGCGCGCGCGCCGCCCCCGCCGCTCCCGTCCAGATCGGAAGAG1205980.10287200190529613No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACACG1199720.10233801400174287No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTG1197040.10210940576188303Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGGAAGAGCACACGTCTCAGAGTTCTACAGTCCGACGATCAGATCG1194720.10191150609155657Illumina DpnII expression Sequencing Primer (100% over 24bp)
AGGGCTTCTGTCGTGAGTGGCACACGCAGAGATCGGAAGAGCACACGTCT1178570.10053388554165481Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTAGATCGGAAGAGCACACGT1174680.10020206238752986No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)