FastQCFastQC Report
Wed 30 Aug 2023
SRR11294608_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11294608_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80155659
Total Bases2.7 Gbp
Sequences flagged as poor quality0
Sequence length18-50
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC22873552.8536413130855802No Hit
AGCCAGGGATTGTGGGTTCGGGTCCCATCTGGGGTGCC19032562.374449943702665No Hit
AGCCAGGGATTGTGGGTTCGTGTCCCATCTGGGGTGCC15497541.9334305516719663No Hit
TCGCTGCGATCTATTGAAA11261421.4049438480694172No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGC10056801.25465876339436No Hit
GGGGATGTAGCTCAGTGGTAGAGCATGC9245051.1533870615423423No Hit
ATCAGAAGATTCCAGGTTCGGCTCCTGGCTGGCTCGCC8749311.0915398998840495No Hit
TCGCTGCGATCTATTGAA8699531.0853294837236631No Hit
ATCTGAGGGTCCAGGGTTCAGGTCCCTGTTCGGGCGCC8184871.0211219147983053No Hit
AGCCAGGGATTGTGGGTTCGGGTCCCACCTGGGGTGCC7100280.8858114434565375No Hit
TTCTGGGCTGTAGTGCGC5918720.738403261084785No Hit
AGCCAGGGATTGTGGGTTCGTGTCCCACCTGGGGTGCC5768930.719715871838818No Hit
ATCAGAAGATTCTAGGTTCGGCTCCTGGCTGGCTCGCC5652880.7052377923809472No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCC5191770.6477109744678164No Hit
ATCAGAAGATTGAGGGTTCGTGTCCCTTCGTGGTCGC4956730.6183880292219917No Hit
ATCAGAAGATTGAGGGTTCGGGTCCCTTCGTGGTCGC4943240.616705053850284No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTT4622240.5766579749534589No Hit
TCAGAAGATTCCAGGTTCGGCTCCTGGCTGGCTCGCC4224510.5270382718704865No Hit
TTCAAAGGTTCCGGGTTCGGGTCCCGGCGGAGTCGCC4046510.5048314804572936No Hit
AGCTGGGGATTGTGGGTTCGGGTCCCATCTGGGTCGCC3903880.4870373531580596No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG3775050.47096487597962355No Hit
AGCCAGGGATTGTGGGTTCGGGTCCCATCTGGGGTGC3715190.4634969066875241No Hit
ATCTGAGGGTCCAGGGTTCAGGTCCCTGTTCGGGCGC3660760.4567063692907821No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGC3495420.4360790047275389No Hit
AGCTGGGGATTGTGGGTTCGGGTCCCATCTGGGTCGC3460060.43166758818613166No Hit
TGTATGAGGCCCCGGGTTCGATCCCCGGCATCTCCACC3437160.42881064704364785No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGT3365240.419838105254677No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT3342080.41694872722585935No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTC3314980.4135678056118284No Hit
ACAGGAGATCCTGGGTTCGAATCCCAGCGGTGCC3227790.4026902205370179No Hit
AGCTGGGGATTGTGGGTTCGTGTCCCATCTGGGTCGCC3206950.4000902793401025No Hit
TTCAAAGGTTCCGGGTTCGTGTCCCGGCGGAGTCGCC3190230.39800433803432395No Hit
AGCCAGGGATTGTGGGTTCGAGTCCCATCTGGGGTGCC3188320.39776605167702506No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC3116200.3887685584370281No Hit
GGGGGTGTAGCTCAGTGGTAGAGCGTGC3078490.38406396234606466No Hit
AACAGGAGATCCTGGGTTCGAATCCCAGCGGTGCC3015890.37625415817490815No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACA2955640.3687375335533078No Hit
AGCCAGGGATTGTGGGTTCGTGTCCCATCTGGGGTGC2949610.36798524730487214No Hit
ATCAGAAGATTGAGGGTTCGGGTCCCTTCGTGGTCGCC2930070.36554749053962615No Hit
ATCAGAAGATTGAGGGTTCGTGTCCCTTCGTGGTCGCC2875280.3587120405310373No Hit
TTCTGGGCTGTAGTGCGCTATGCCG2849930.3555494441134842No Hit
TCAGAAGATTGAGGGTTCGTGTCCCTTCGTGGTCGC2818280.35160087698861037No Hit
AGCTGGGGATTGTGGGTTCGTGTCCCATCTGGGTCGC2810580.3506402461241071No Hit
ATCAGAAGATTCCAGGTTCGTCTCCTGGCTGGCTCGCC2746050.3425896604505491No Hit
TGCACGAGGCCCCGGGTTCAATCCCCGGCACCTCCACC2725640.34004336487333975No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGC2716290.33887688453787146No Hit
ATCTCAGGGTCGTGGGTTCGTGCCCCACGTTGGGCGCC2618480.32667437741357724No Hit
ATCAGAAGATTCCAGGTTCGACTCCTGGCTGGCTCGCC2552120.31839548596313083No Hit
TCAGAAGATTGAGGGTTCGGGTCCCTTCGTGGTCGC2524060.31489479738417475No Hit
ATCAGAAGATTCCAGGTTCGGCTCCTGGCTGGCTCGC2521810.31461409356013154No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGCACC2521590.3145866469640029No Hit
AACAGGAGATCCTGGGTTCGTATCCCAGCGGTGCC2519400.3143134285752675No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTT2513110.313528705440498No Hit
ATCTCAGGGTCGTGGGTTCGGGCCCCACGTTGGGCGCC2454920.3062690807644661No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAC2447220.3053084498999628No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCC2372540.2959915780868323No Hit
TCAGAAGATTCTAGGTTCGGCTCCTGGCTGGCTCGCC2358210.29420380662081513No Hit
TTCTGGGCTGTAGTGCGCTATGCC2106150.26275749289267275No Hit
ACAGGAGATCCTGGGTTCGTATCCCAGCGGTGCC2105860.262721313288685No Hit
TCTGAGGGTCCAGGGTTCAGGTCCCTGTTCGGGCGCC2098230.2617694154320408No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGC2076590.2590696684310212No Hit
TCTCGGCGCCCCCTCGATGCTC2074790.2588451053717867No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTT2053950.2562451641748713No Hit
TGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT2039630.2544586402814054No Hit
GGGGATGTAGCTCAGTGGTAGAGCCATGC2019460.25194228644542743No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCC1996000.24901548124007067No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCT1963380.2449458995777204No Hit
ATCAGAAGATTGCAGGTTCGGGTCCTGCCGCGGTCGCC1866460.23285442641049212No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC1800110.224576782532597No Hit
AGCCAGGGATTGTGGGTTCGGGTCCCACCTGGGGTACC1710430.21338855189251207No Hit
TTCAAAGGTTCCGGGTTCGGGTCCCGGCGGAGTCGC1655930.20658928148791092No Hit
TCAGAAGATTCCAGGTTCGTCTCCTGGCTGGCTCGCC1652610.20617508740087837No Hit
ATTCAAAGGTTCCGGGTTCGGGTCCCGGCGGAGTCGCC1645060.2052331701246446No Hit
TCAGAAGATTGAGGGTTCGTGTCCCTTCGTGGTCGCC1634040.20385834517310875No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGG1610980.2009814428698041No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTG1570820.19597119150377143No Hit
GGGGATGTAGCTCAGTGGTAGAGCGCATGC1558830.19447535201475918No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGT1558030.19437554621065495No Hit
CAGCCAGGGATTGTGGGTTCGGGTCCCATCTGGGGTGCC1538500.19193903701796028No Hit
TCGCTGCGATCTATTGAAAGT1520590.18970463457857667No Hit
ATCAGAAGATTCTAGGTTCGTCTCCTGGCTGGCTCGCC1519670.1895898579038568No Hit
CAGGAGATCCTGGGTTCGAATCCCAGCGGTGCC1506450.18794056699103429No Hit
TCAGAAGATTGAGGGTTCGGGTCCCTTCGTGGTCGCC1470370.1834393252259332No Hit
ATCAGAAGATTGCAGGTTCGTGTCCTGCCGCGGTCGCC1458160.1819160391407923No Hit
ATCAGAAGATTGCAGGTTCGGGTCCTGCCGCGGTCGC1454370.18144320914384848No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCG1447600.18059860252661636No Hit
ACCGAAAGGTTGGTGGTTCGTGCCCACCCAGGGACGC1397020.174288380562126No Hit
AGCCAGGGATTGTGGGTTCGTGTCCCACCTGGGGTACC1378880.17202528395406244No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTC1371460.17109958512099563No Hit
ATCTAAAGGTCCCTGGTTCGTTCCCGGGTTTCGGC1347880.16815780904502325No Hit
AGCCAGGGATTGTGGGTTCGAGTCCCACCTGGGGTGCC1337130.16681666855237257No Hit
CCAGGAGATCCTGGGTTCGAATCCCAGCGGTGCC1306620.16301032469834725No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGCACC1305300.16284564512157526No Hit
ATCAGAAGATTGAGGGTTCGAGTCCCTTCGTGGTCGC1291340.16110403383995633No Hit
TTCAAAGGTTCCGGGTTCGTGTCCCGGCGGAGTCGC1283850.1601696019990304No Hit
ATCAGAAGATTCTAGGTTCGGCTCCTGGCTGGCTCGC1270760.15853652952937486No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTG1254140.1564630639491093No Hit
TCAGAAGATTCCAGGTTCGACTCCTGGCTGGCTCGCC1250630.15602516598360197No Hit
TCAGAAGATTCTAGGTTCGTCTCCTGGCTGGCTCGCC1241380.15487116137364673No Hit
ATTCAAAGGTTCCGGGTTCGTGTCCCGGCGGAGTCGCC1237940.15444199641599854No Hit
CAGCCAGGGATTGTGGGTTCGTGTCCCATCTGGGGTGCC1231220.15360362766152294No Hit
TCAGAAGATTGCAGGTTCGGGTCCTGCCGCGGTCGCC1230380.15349883156721347No Hit
CAGGAGATCCTGGGTTCGTATCCCAGCGGTGCC1228730.1532929820962485No Hit
ATCTAAAGGTCCCTGGTTCGTTCCCGGGTTTCGGCACC1220310.1522425260080514No Hit
TTCTGGGCTGTAGTGCGCT1196550.14927829362615558No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGC1175710.1466783524292402No Hit
GGGGATGTAGCTCAGTGGTAGAGCTCATGC1152540.14378772682787125No Hit
ATCAGAAGATTGCAGGTTCGTGTCCTGCCGCGGTCGC1137460.1419063874205064No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCC1126780.14057397993571483No Hit
TGGGCGCGCGCCGCGGCTGGACG1120950.13984664513830522No Hit
ATCTAAAGGTCCCTGGTTCGTTCCCGGGTTTCGG1068820.1333430494283629No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCG1045210.13039753063473658No Hit
AACCAGGGGTCGCGAGTTCATTTCTCGCTGGGGCC1039810.129723841457033No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCG1029870.12848375434103787No Hit
ATCTGAGGGTCCAGGGTTCATGTCCCTGTTCGGGCGCC992820.12386149803846039No Hit
TCAGAAGATTGCAGGTTCGTGTCCTGCCGCGGTCGCC984620.12283848854639197No Hit
ATCTGAGGGTCCAGGGTTCAGGTCCCTGTTCGGGCGCCA976640.12184292565045221No Hit
ATTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGC960880.11987675130959874No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCC959010.1196434552425051No Hit
TCAGAAGATTCCAGGTTCGGCTCCTGGCTGGCTCGC958350.11956111545411909No Hit
AGCCAGGGATTGTGGGTTCGTCCCATCTGGGGTGCC944410.11782199931760276No Hit
TCTGAGGGTCCAGGGTTCAGGTCCCTGTTCGGGCGC915830.11425643696597891No Hit
TCCCTGGTCTAGTGGTTAGGATTCGGCGC912370.11382477686322809No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCC904850.11288660230464825No Hit
ATTCAAAGGTTCCGGGTTCGGGTCCCGGCGGAGTCGC904620.11285790813596829No Hit
TCAGAAGATTGCAGGTTCGGGTCCTGCCGCGGTCGC892450.11133961234103258No Hit
TTCAAAGGTTCCGGGTTCGAGTCCCGGCGGAGTCGCC879720.10975145248322393No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGC875100.10917507396452195No Hit
AACAGGAGATCCTGGGTTCGGATCCCAGCGGTGCC874300.10907526816041771No Hit
GCGTTGGTGGTATAGTGGTGAGCATAGCTGCC855420.10671985118355773No Hit
ATCGGTCGTCCGACCTGGGTATAGGGGCG855330.10670862303059601No Hit
ATCAGAAGATTCTAGGTTCGACTCCTGGCTGGCTCGCC825290.10296091508648192No Hit
AGCCAGGGATTGTGGGTTCGGGTCCCACCTGGGGTGC824990.10292348790994282No Hit
TGTATGAGGTCCCGGGTTCGATCCCCGGCATCTCCACC814440.10160729886831821No Hit
ATCAGAAGATTGAGGGTTCGAGTCCCTTCGTGGTCGCC810680.10113821158902829No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGG802350.10009898365379293No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)