FastQCFastQC Report
Wed 30 Aug 2023
SRR11362451.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11362451.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1054509
Total Bases105.4 Mbp
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC132891.260207357168123Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTCCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC60570.5743905457421417Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCCCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC54720.5189144900612512Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCCTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC24820.23537020547003393Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC14900.14129798797354978Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTCTACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCCA14870.14101349538031444Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAATCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC14280.1354184743800195Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CACTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC14250.13513398178678418Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC14190.1345649966003135Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC14190.1345649966003135Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAACCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC14150.1341856731426664Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCCTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC13680.1297286225153128Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AATCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC13670.12963379165090105Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CACCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC13580.128780313871195Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACACCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC13490.12792683609148903Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACATCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC13450.12754751263384193Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACCTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC13400.12707335831178304Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCACCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC13330.12640954226090056Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACCCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC13170.12489224843031212Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAATCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC13160.12479741756590033Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TACTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC13140.12460775583707677Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACTTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC12990.1231852928709001Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCCA12900.1223318150911941Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AATTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC12830.12166799904031166Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATACCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC12610.11958172002325253Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACTCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC12540.11891790397237008Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTACCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC12380.11740061014178162Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CATCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC12140.11512466939589895Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAACCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC12130.11502983853148717Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAATCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC12130.11502983853148717Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATATCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC11880.1126590669211927Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCCCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC11460.10867617061589802Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCATCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC11310.10725370764972135Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCTTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC11300.10715887678530957Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CATTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC11210.10630539900560357Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATTTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC10940.10374496566648556Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TACCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC10840.10279665702236775Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCTCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC10790.10232250270030886Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC10730.10175351751383818Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC10700.10146902492060286Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC10570.10023622368324973Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)