FastQCFastQC Report
Wed 30 Aug 2023
SRR11362455.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11362455.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1803204
Total Bases180.3 Mbp
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC562093.117173653119669Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCCCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC337481.871557516509502Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTCCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC254871.4134285416403247Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCCTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC200121.1098023296310344Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTTTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC98430.5458616995082087Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTACCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC86580.48014534129249936Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTATCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC79200.4392181916189183Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTTCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC76260.42291387996033725Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTCTACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCCA56560.31366390047936893Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATCCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC50790.2816653024283442Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCCA50070.27767240977726315Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCCA45750.2537150538707767Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GACCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC45280.2511085822790987Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC42110.2335287632458668Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCCA41790.23175414428983077Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAATCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC39530.21922089791282626Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC39250.21766810632629477Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACACCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC39050.21655896947877223Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AATCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC38180.21173422419204926Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAACCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC38090.21123511261066413Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC38000.210736001029279Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CACTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC36690.20347115467800647Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCACCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC36280.20119742414058533Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACATCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC36210.20080922624395245Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CACCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC36160.2005319420320718Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAATCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC35540.19709361780475199Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATACCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC35470.1967054199081191Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CATCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC35260.19554082621822044Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AATTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC35150.19493080095208307Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACCCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC34460.19110427882813036Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACCTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC34370.19060516724674523Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAACCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC34270.19005059882298397Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTACCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC34270.19005059882298397Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCCTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC33970.18838689355170019Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCATCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC33600.18633499038378354Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACTTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC33550.1860577061719029Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TACTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC33060.18334032089547272Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAATCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC33050.1832848640530966Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACTCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC33050.1832848640530966Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TACCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC32820.1820093566784457Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCCCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC32720.18145478825468442Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATATCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC32500.18023473772240967Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CATTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC32070.17785009350023626Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTTTACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCCA32010.1775173524459795Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATCTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC31030.17208258189311915Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATTTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC30970.17174984083886238Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTGTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC30920.17147255662698174Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCTTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC30570.16953156714381734Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCTCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC29680.16459590817234213Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTGCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC29630.16431862396046149Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC29570.16398588290620475Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATTCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC29390.16298765974343446Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTACCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC29260.16226672079254484Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTATCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC28930.16043664499413268Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTACACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCCA28900.1602702744670043Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTTTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC28690.15910568077710563Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC28350.15722014813631735Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC27930.15489096075652006Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCACCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC27640.1532827123276124Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTTCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC27620.15317179864286015Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC27050.15001075862742097Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTATCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC26940.1494007333612836Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC26830.1487907080951462Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCATCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC26690.14801431230188042Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC26400.14640606387297278Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCTTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC26010.14424324702030386Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC25270.14013944068447054Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTTTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC24410.1353701522401237Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGACCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC23370.12960264063300658Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCTCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC23170.12849350378548408Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC22960.12732891009558542Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGATCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC22270.12350238797163271Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GAACCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC22210.12316964691737595Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGATCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC22050.12228233743935793Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GAATCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC22040.12222688059698181Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGACCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC21900.12145048480371606Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC21540.11945403847817551Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTTCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC21280.11801216057639623Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGATCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC21050.11673665320174532Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTATACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCCA21020.11657028267461696Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGCTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC20970.11629299846273632Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GACTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC20890.11584934372372731Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTC20870.11573843003897506Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGCTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC20530.11385289739818678Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGACCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC20520.11379744055581066Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCACCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC19820.10991546158948184Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGCTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC19760.10958272053522508Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAGTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC19630.10886178158433543Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCTTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC19240.10669896473166653Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGCCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC19010.10542345735701562Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAGCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC18980.10525708682988726Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GATTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC18630.10331609734672283Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC18590.10309426997721834Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGTTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC18040.10004414364653139Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph