FastQCFastQC Report
Wed 30 Aug 2023
SRR11362466.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11362466.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34841979
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC379910.10903800843229945Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC359080.10305958797575762Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAATCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC355310.10197755988544738Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)