FastQCFastQC Report
Wed 30 Aug 2023
SRR11362467.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11362467.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6377675
Total Bases318.8 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC285160.4471221879446664Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAATCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC271970.4264406699933753Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC266710.41819315032515764Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CACTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC265360.41607639147494974Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACATCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC263570.41326972603652584Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAATCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC261940.41071393572108955Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAACCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC261100.40939684132540466Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAACCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC259530.40693512918108865Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TACTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC258680.4056023550902171Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACACCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC256400.40202738458764364Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAATCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC253060.3967903663952773Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATACCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC249660.3914592700317906Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC249280.39086344161469505Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AATCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC246510.386520166047972Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AATTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC245850.38548530616564813Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACTTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC242750.3806246006577632Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATATCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC238770.3743840819734464Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CATTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC236720.3711697444601677Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTACCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC235290.36892754804846595Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCACCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC234640.36790836786132874Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCATCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC233790.36657559377045706Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACCTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC233730.3664815155993368Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACTCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC230430.36130721618771733Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTATCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC229750.36024099691502Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CACCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC227580.3568385030595005Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TACCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC226980.355897721348297Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC222610.3490456945516979Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCCTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC222200.3484028270490422Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CATCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC220160.34520416923095015Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCATCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC219420.34404387178713247Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTACCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC218420.34247590226845986Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCACCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC218100.34197415202248466Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATTTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC217930.3417075972043103Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTATCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC214950.33703504803866613Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC210880.3306534120976688Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCTTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC207810.3258397456753441Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTTTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC205860.3227822051139326Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC205810.3227038066379989Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCTTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC202310.31721591332264504Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC202070.31683960063816363Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC198090.31059908195384683Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACCCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC197590.30981509719451056Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATTCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC191740.30064247551027606Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTTTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC189590.2972713410451301Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCTCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC184100.28866318838761773Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCTCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC178520.2799139184734249Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCCCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC174430.2735009231420541Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTTCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC173670.27230926630786295Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GAATCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC171800.26937716330794526Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC167020.26188226900869044Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GAACCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC159870.2506712869501817Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTTCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC155780.24425829161881094Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC153410.240542203859557Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGCTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC152640.2393348673301791Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGATCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC151630.23775121811631983Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGATCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC150490.23596373286503308Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGACCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC149400.23425464608968002Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGACCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC148700.23315706742660924Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC143770.22542697769955353Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGATCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC143770.22542697769955353Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGCTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC143200.22453323507391015Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GACTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC142850.22398444574237478Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGACCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC139990.21950005291897123Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGTTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC138870.21774392705805798Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GATTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC137790.21605051997789163Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAGTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC135080.211801322582289Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACGTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC134410.21075078300477837Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGTTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC132210.20730125006369876Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC127890.20052762174303332Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC126370.19814430807465105Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTATCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC125550.19685857306933952Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC122670.19234282085556256Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAGTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC121260.19013198383423427Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATGTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC117190.18375034789323696Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC112470.17634953176510249Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCTTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC109140.1711281932679229Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTC107930.16923095015032907Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCACCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC107790.1690114344177149Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTGTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC105870.1660009329418636Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCGTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC104570.16396257256758928Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC101710.15947817974418577Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTACCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC101590.15929002340194506Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAGCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC100390.157408459979538Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC96340.15105818342891414Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTGTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC94660.14842399463754424Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC93010.14583684493173454Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTTTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC92820.14553893072318674Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACGCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC90490.1418855617446797Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGCCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC89140.13976880289447172Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GACCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC87980.13794995825281156Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GATCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC85940.13475130043471953Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC84750.1328854167074992Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAGCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC77730.12187827068641786Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGATCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC75940.11907160524799397Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GAGTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC75050.11767611237637539Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC74550.11689212761703913Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGGTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC74330.11654717432293116Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCTCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC74200.11634333828550374Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGCCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC71780.11254885205031614Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATGCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC68860.10797038105579226Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCGCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC67370.10563410647297015Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC64660.10138490907736752Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph