FastQCFastQC Report
Wed 30 Aug 2023
SRR11362468.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11362468.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7550171
Total Bases377.5 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC308890.4091165617308535Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC279490.3701770463212025Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACACCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC278250.3685346994127683Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAATCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC277110.36702479983565933Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAACCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC275420.36478643993626103Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAACCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC272630.361091159392284Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CACTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC271650.35979317554529555Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATACCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC271320.359356099351922Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACATCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC268160.3551707636820411Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAATCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC265580.3517536225338473Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AATCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC265200.35125032267481093Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TACTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC261690.34660142134529137Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAATCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC260370.34485311657179685Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AATTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC258130.34188629635010914Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACTTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC258080.3418200726844465Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CATTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC254070.33650893469830023Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATATCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC253040.3351447271856492Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC252200.33403216960251625Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCACCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC249400.3303236443254067Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTACCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC248440.32905214994468335Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCATCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC247790.3281912422910686Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACTCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC246610.32662836378142956Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTATCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC243520.32253574124347645Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATTTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC239410.31709215592600487Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTACCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC239190.3168007717970891Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CATCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC237220.31419155936997983Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACCTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC234990.31123798388142465Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC231800.3070129140121462Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTATCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC231230.30625796422359175Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TACCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC229740.3042844989868441Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CACCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC228650.3028408230753979Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCCTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC228290.3023640126826267Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCACCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC227870.30180773389106025Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC227050.3007216657741924Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCATCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC225030.2980462296814204Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTTTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC220310.29179471564286424Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCTTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC216480.2867219828531036Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC214330.28387436522960874Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC213990.28342404430310253Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATTCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC210510.2788148771729806Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCTTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC209330.27725199866334155Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC206790.2738878364476778Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTTTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC201520.2669078620868322Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCTCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC198130.262417897554903Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACCCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC197680.261821884563939Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCTCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC190210.2519280689139358Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTTCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC185020.24505405241815054Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GAATCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC176760.23411390285067715Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GAACCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC175200.2320477244820018Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCCCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC170950.22641871290067472Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTTCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC170610.22596839197416854Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC167800.2222466219639264Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGACCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC167620.2220082167675408Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGATCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC162550.21529313706934583Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC160080.21202168798560983Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGCTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC159840.21170381439042904Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGACCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC157490.20859130210428345Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGATCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC157180.2081807153771749Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAGTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC153630.2034788351151252Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACGTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC152840.202432501197655Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGATCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC150380.19917429684705154Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GACTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC149240.19766439726994264Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGACCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC149100.19747897100608713Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GATTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC148280.19639290288921935Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGCTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC148170.19624721082476146Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGTTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC147920.1959160924964481Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC142680.1889758523350001Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC142040.1881281894145179Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGTTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC141150.18694940816572234Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAGTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC135500.17946613394584043Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTATCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC134360.1779562343687315Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC133660.17702910304945413Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATGTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC131790.1745523379536702Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC129010.17087030214282564Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC124990.16554591942354685Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCACCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC120600.15973148157836425Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCGTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC119500.15827456093378547Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCTTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC117650.15582428530426662Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTGTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC116240.15395677793257928Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAGCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC109040.14442057007715453Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC105720.14002331867715315Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTGTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC105620.1398908713458278Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTACCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC103460.1370300089892004Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTTTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC102250.13542739628016373Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC101210.13404994403438014Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACGCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC99620.1319440314663072Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC95630.12665938294642598Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GATCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC94630.12533490963317256Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GACCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC93220.12346740226148521Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGCCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC93190.12342766806208759Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTC90140.11938802445666463Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGCCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC86620.11472587839401252Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GAGTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC83910.11113655571509573Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAGCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC83820.11101735311690292Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGATCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC81300.10767968036750426Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGGTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC80830.10705717791027515Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC78300.10370626042774396Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCTCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC78040.10336189736629806Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph