FastQCFastQC Report
Wed 30 Aug 2023
SRR11362469.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11362469.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9746667
Total Bases487.3 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC259730.266480839039643Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC245590.25197331559598785Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CACTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC238970.24518125016479994Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAATCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC238560.24476059354443935Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAACCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC236370.24251367159665965Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAACCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC235320.24143638025183378Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACACCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC235200.24131326124099653Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TACTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC232960.239015039705368Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC231260.23727085371850706Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACATCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC230890.23689123676842555Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAATCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC228180.2341107991070178Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATACCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC227120.23302324784462217Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AATCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC223730.22954513578847005Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AATTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC223350.22915525892081878Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAATCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC220570.22630300183642266Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACTTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC217900.22356360384529397Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCACCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC212180.2176949309953854Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CATTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC211730.21723323470474576Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCATCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC211280.21677153841410607Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTACCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC210000.2154582689651755Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATATCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC208870.21429889827979143Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACTCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC207490.21288302965516312Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CACCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC205230.21056428828439508Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACCTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC204760.21008207215861585Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTATCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC203540.20883036221510387Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TACCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC201460.20669629936059167Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATTTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC197740.20287961002463714Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CATCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC196740.20185361826766013Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC196180.20127906288375297Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTACCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC195590.20067372774713654Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCACCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC192480.19748289338293798Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCCTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC191880.19686729832875177Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCATCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC188420.19331736684961126Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTATCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC188110.1929993094049484Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC185760.1905882287760524Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTTTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC185410.1902291316611104Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCTTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC182840.18759233284567944Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC181960.18668946009953968Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCTTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC179970.18464773650315539Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC178690.1833344670542248Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACCCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC176890.18148768189166614Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC175420.1799794740089099Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATTCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC172760.17725033593535103Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTTTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC167360.17170998044767508Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCTCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC164380.16865252501188355Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCTCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC156020.1600752339235556Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTTCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC154550.15856702604079936Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GAATCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC153930.15793091115147365Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCCCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC151850.15579684829696142Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GAACCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC145860.14965115767266904Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC141720.14540355179878414Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGCTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC141470.14514705385953988Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGACCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC139460.14308481042801607Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC139170.1427872728184927Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGATCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC135930.13946305952588714Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTTCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC135580.1391039624109452Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGATCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC133420.13688782021587484Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGACCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC131000.13440492016399042Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GACTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC127550.1308652486024197Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGCTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC127290.13059849074560564Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTC127030.13033173288879163Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGACCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC126350.12963405849404724Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGATCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC125200.12845416797352366Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACGTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC124640.12787961258961653Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC124160.12738713654626754Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GATTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC123630.12684336091506973Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGTTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC123490.12669972206909294Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAGTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC121540.12469903814298776Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC117360.12041039259882379Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGTTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC116700.11973323803921895Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTATCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC114990.11797879213478824Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC110380.11324897013512414Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATGTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC106670.10944254071673938Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC106490.1092578622004835Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAGTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC106280.10904240393151833Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC99510.10209643973678385Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCACCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC98500.10106018806223707Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)