FastQCFastQC Report
Tue 18 Jul 2023
SRR11453099_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11453099_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47733393
Total Bases3.1 Gbp
Sequences flagged as poor quality0
Sequence length61-75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGAATGTAGC3718930.779104473046783No Hit
TTTTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGAATGTAG3332300.6981066692661048No Hit
ATTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGAATGTAGC3324110.6963908892879247No Hit
CTTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGAATGTAGC3020490.6327834268978113No Hit
ACTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGAATGTAGC2525530.5290908190834036No Hit
ATTTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGAATGTAG2511720.5261976662752635No Hit
TCTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGAATGTAGC2388820.5004504917553211No Hit
ACGTTTAGTGGTAAAATCCAACGTTGCCATCGTTGGGCCCCCGGTTCGAT2206050.4621607351482431No Hit
CTTTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGAATGTAG2160030.4525196857470408No Hit
TTACGGACCAAGGAGTCTAACGTCTATGCGAGTGTTTGGGTGTAAAACCC2073860.4344673340108045No Hit
GTTTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGAATGTAG2062750.43213982295371295No Hit
TCTTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGAATGTAG2017990.42276273970299993No Hit
TGTTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGAATGTAG1989970.4168926353087869No Hit
GTTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGAATGTAGC1971790.4130839808517278No Hit
CCTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGAATGTAGC1791840.3753850056290782No Hit
ATACGGACCAAGGAGTCTAACGTCTATGCGAGTGTTTGGGTGTAAAACCC1775780.37202048469506455No Hit
TCACGGACCAAGGAGTCTAACGTCTATGCGAGTGTTTGGGTGTAAAACCC1711560.3585665908979066No Hit
CTGTTTAGTGGTAAAATCCAACGTTGCCATCGTTGGGCCCCCGGTTCGAT1685600.35312805021004895No Hit
TCGTTTAGTGGTAAAATCCAACGTTGCCATCGTTGGGCCCCCGGTTCGAT1662430.3482740059982746No Hit
ATGTTTAGTGGTAAAATCCAACGTTGCCATCGTTGGGCCCCCGGTTCGAT1645270.34467903842494496No Hit
ACTTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGAATGTAG1629460.3413668917271395No Hit
TGTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGAATGTAGC1595700.33429427486958657No Hit
TGACGGACCAAGGAGTCTAACGTCTATGCGAGTGTTTGGGTGTAAAACCC1540130.3226525296452318No Hit
GTACGGACCAAGGAGTCTAACGTCTATGCGAGTGTTTGGGTGTAAAACCC1514430.3172684581630307No Hit
CTACGGACCAAGGAGTCTAACGTCTATGCGAGTGTTTGGGTGTAAAACCC1508260.3159758620134127No Hit
TTGTTTAGTGGTAAAATCCAACGTTGCCATCGTTGGGCCCCCGGTTCGAT1355120.2838934998817285No Hit
AGTTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGAATGTAG1339860.28069657650358104No Hit
TATTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGAATGTAG1312560.2749773099096475No Hit
ACACGGACCAAGGAGTCTAACGTCTATGCGAGTGTTTGGGTGTAAAACCC1297030.27172382235639525No Hit
CCTTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGAATGTAG1290420.27033904755105087No Hit
ACGGCGCCAGTGAAATACCACTACCTTTATAGTTTCTTTACTTATTCAAT1216160.25478180442777243No Hit
ACGGTAAGTATTATAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGACT1179770.24715821060530938No Hit
TAACGGACCAAGGAGTCTAACGTCTATGCGAGTGTTTGGGTGTAAAACCC1170760.2452706431323665No Hit
AGTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGAATGTAGC1158520.24270640052761386No Hit
GCTTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGAATGTAG1127780.23626646444345578No Hit
GCTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGAATGTAGC1111010.23275320067861088No Hit
GCACGGACCAAGGAGTCTAACGTCTATGCGAGTGTTTGGGTGTAAAACCC1103190.23111493457001897No Hit
AGACGGACCAAGGAGTCTAACGTCTATGCGAGTGTTTGGGTGTAAAACCC1077520.22573714799616276No Hit
CCGTTTAGTGGTAAAATCCAACGTTGCCATCGTTGGGCCCCCGGTTCGAT1071370.2244487417854415No Hit
CCACGGACCAAGGAGTCTAACGTCTATGCGAGTGTTTGGGTGTAAAACCC1070680.2243041888935069No Hit
AATTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGAATGTAG1046930.21932863645372958No Hit
GGTTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGAATGTAG1033340.21648157297345277No Hit
TTTACGGACCAAGGAGTCTAACGTCTATGCGAGTGTTTGGGTGTAAAACC953020.19965477836448794No Hit
GGACGGACCAAGGAGTCTAACGTCTATGCGAGTGTTTGGGTGTAAAACCC951210.1992755888943407No Hit
CGTTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGAATGTAG939910.19690827341773084No Hit
AAACGGACCAAGGAGTCTAACGTCTATGCGAGTGTTTGGGTGTAAAACCC906710.18995297484928425No Hit
TATGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGAATGTAGC901670.18889711024732728No Hit
ATTACGGACCAAGGAGTCTAACGTCTATGCGAGTGTTTGGGTGTAAAACC860150.18019879709787234No Hit
CGACGGACCAAGGAGTCTAACGTCTATGCGAGTGTTTGGGTGTAAAACCC829530.17378400064709418No Hit
AATGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGAATGTAGC774630.16228261837577732No Hit
GCGTTTAGTGGTAAAATCCAACGTTGCCATCGTTGGGCCCCCGGTTCGAT758740.15895371192238525No Hit
CATTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGAATGTAG751330.15740133956117472No Hit
CTTACGGACCAAGGAGTCTAACGTCTATGCGAGTGTTTGGGTGTAAAACC736220.15423584072475216No Hit
GTGTTTAGTGGTAAAATCCAACGTTGCCATCGTTGGGCCCCCGGTTCGAT697660.14615763853200212No Hit
GGTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGAATGTAGC693710.14533012559991282No Hit
CAACGGACCAAGGAGTCTAACGTCTATGCGAGTGTTTGGGTGTAAAACCC684030.14330219517393203No Hit
CGTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGAATGTAGC680210.14250191684467098No Hit
TCTACGGACCAAGGAGTCTAACGTCTATGCGAGTGTTTGGGTGTAAAACC666650.13966113827273918No Hit
TCGGTAAGTATTATAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGACT646990.13554242833732771No Hit
GATTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGAATGTAG644210.13496002683069272No Hit
TGTACGGACCAAGGAGTCTAACGTCTATGCGAGTGTTTGGGTGTAAAACC640260.13413251389860345No Hit
GAACGGACCAAGGAGTCTAACGTCTATGCGAGTGTTTGGGTGTAAAACCC638340.1337302797645246No Hit
GTTACGGACCAAGGAGTCTAACGTCTATGCGAGTGTTTGGGTGTAAAACC614880.12881548143874877No Hit
TCGGCGCCAGTGAAATACCACTACCTTTATAGTTTCTTTACTTATTCAAT613010.12842372215191156No Hit
TTTTTAGTGGTAAAATCCAACGTTGCCATCGTTGGGCCCCCGGTTCGATT602090.12613601551433815No Hit
ACTACGGACCAAGGAGTCTAACGTCTATGCGAGTGTTTGGGTGTAAAACC590810.12377288997662496No Hit
CTGGCGCCAGTGAAATACCACTACCTTTATAGTTTCTTTACTTATTCAAT571600.11974845366638824No Hit
TGGTTTAGTGGTAAAATCCAACGTTGCCATCGTTGGGCCCCCGGTTCGAT550430.11531340334427934No Hit
CATGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGAATGTAGC548080.11482108552392242No Hit
ACGTCAGAATGGGCGCTTGTCGCGTGCCAGATCGGGGTTCAATTCCCCGT545990.11438323690922202No Hit
CCGGCGCCAGTGAAATACCACTACCTTTATAGTTTCTTTACTTATTCAAT532110.11147541931494373No Hit
CTGGTAAGTATTATAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGACT527040.1104132698046418No Hit
CTGTCAGAATGGGCGCTTGTCGCGTGCCAGATCGGGGTTCAATTCCCCGT506060.10601802390205113No Hit
CCGGTAAGTATTATAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGACT503850.10555503565397079No Hit
ATGTCAGAATGGGCGCTTGTCGCGTGCCAGATCGGGGTTCAATTCCCCGT501840.10513394679485702No Hit
TCGTCAGAATGGGCGCTTGTCGCGTGCCAGATCGGGGTTCAATTCCCCGT501020.10496215930009417No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)