FastQCFastQC Report
Wed 30 Aug 2023
SRR11459635_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11459635_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54108012
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length18-45
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG557980910.312352632730251No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG20691503.824110189078837No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG16357243.0230717033181707No Hit
AAGGCGAGCGAGAAAACCAATGTTAAGGAACTC15025992.7770360515185812No Hit
AAGGCGAGCGAGAAAACCAATGTTAAGG13499042.4948320038074954No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG11758582.1731679958968No Hit
AAGGCGAGCGAGAAAACCAATGTTAAGGAACT8001011.4787107683793668No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG7666311.4168530161485142No Hit
GGAGTGGACGTACCGCTGGTGTTCCAGTT7126981.3171764654742812No Hit
CGCTCAACGGATAAAAGCTACCCCGGGG6859401.2677235304819552No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTC6642141.2275705121082623No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTC6002381.1093329394545117No Hit
TAAGGCGAGCGAGAAAACCAATGTTAAGGAACTC4747960.8774966635255421No Hit
AGGCGAGCGAGAAAACCAATGTTAAGG4550430.8409900552250931No Hit
AGGGCTTCTGTCGTGAGTGGCACACGTAGG4023950.7436883838940525No Hit
GTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG3943920.7288975983815483No Hit
AGGGCTTCTGTCGTGAGTGGCACACGTAGGG3063020.5660936129015421No Hit
GGGAGATACCATGATCACGAAGGTGGT2867490.5299566356272709No Hit
GGAGTGGACGTACCGCTGGTGTTCCAGT2862390.5290140765105175No Hit
AGGATTCAACCCGGCGGCGGGTCCGGCCGTGTCGG2776930.5132197427619407No Hit
CGGAGTGGACGTACCGCTGGTGTTCCAGTT2720570.5028035404442507No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGAGG2642300.48833803023478295No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCAAGG2557350.4726379523978814No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGAGG2489750.4601444237130723No Hit
CGGCCACACTGGGACTGAGATACGGCCCAGA2433350.4497208287748587No Hit
GGAGTGGACGTACCGCTGGTGTTCCAGTTGT2422140.44764904687313223No Hit
TAAGGCGAGCGAGAAAACCAATGTTAAGGAACT2393720.4423965899911459No Hit
AAGGCGAGCGAGAAAACCAATGTTAAGGA2367890.43762280528805975No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCAAGG2344540.43330736305743406No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGAGG2193170.4053318388411683No Hit
TGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG2002450.3700838241848546No Hit
TAAGGCGAGCGAGAAAACCAATGTTAAGG1959750.3621922017759588No Hit
AGGCGAGCGAGAAAACCAATGTTAAGGAACT1947430.35991527465470363No Hit
TCTTGAGTTGTGATGCATAGTGAAG1897070.35060796541554695No Hit
GACTGCATAATTTGTGGTAGTGGGGG1890260.34934937177141157No Hit
GGGCTTCTGTCGTGAGTGGCACACGTAGG1872900.3461409744641884No Hit
AAGGCGAGCGAGAAAACCAATGTTAAGGAAC1832850.33873911316497823No Hit
AGGCGAGCGAGAAAACCAATGTTAAGGAACTC1794650.3316791605649825No Hit
ATGTACACGGAGTCGACCCAACGCGTA1782570.32944658916686864No Hit
AGGGGAGATACCATGATCACGAAGGTGGT1761940.3256338451318448No Hit
GAGTGGACGTACCGCTGGTGTTCCAGTTGT1690950.31251379185766426No Hit
CGCTCAACGGATAAAAGCTACCCCGGGGA1680880.3106526996408591No Hit
GGGCTTCTGTCGTGAGTGGCACACGTAGGG1618910.29919968229474037No Hit
GGGAGCGTAGCTCAGATGGTTAGAGCACACG1450950.26815806871632986No Hit
ACGGCCACACTGGGACTGAGATACGGCCCAGA1443600.2667996746951265No Hit
GAGTGGACGTACCGCTGGTGTTCCAGTT1435730.2653451766071169No Hit
CGGAGTGGACGTACCGCTGGTGTTCCAGT1321320.2442004337546166No Hit
GGCAGGATCAACCAGGTAGGTAGG1275040.2356471718088626No Hit
CTAAGGCGAGCGAGAAAACCAATGTTAAGGAACTC1235700.22837652952394555No Hit
TGGCAGGGGAGATACCATGATCACGAAGGTGGT1181340.21832995823243329No Hit
TAGACTACGAGGTTGATAGGTTGGAAGTGTAAGTG1174150.21700113469332416No Hit
ATCTTGAGTTGTGATGCATAGTGAAG1165420.21538769526405813No Hit
AGGCGAGCGAGAAAACCAATGTTAAGGAAC1101530.2035798321328087No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGCGGAGG1100510.20339132030945806No Hit
TCGCTCAACGGATAAAAGCTACCCCGGGG1097930.20291449628568872No Hit
CTAAGGCGAGCGAGAAAACCAATGTTAAGG1078570.19933646795228774No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTC1074760.19863232084741905No Hit
GAGTGGACGTACCGCTGGTGTTCCAGT1044240.19299175138794603No Hit
AGTATGGGCCGGCGAGTTATGTTAATATGCAAGGTT1015120.18760992364679746No Hit
CGGCCACACTGGGACTGAGATACGGCCAAGG997380.18433129644460047No Hit
AAGGCGAGCGAGAAAACCAATGTTAAGGAA990910.18313554007491534No Hit
AGGCGAGCGAGAAAACCAATGTTAAGGA952010.17594621661575738No Hit
GGCTTGGTCTAGGGGTATGATTCTC944550.17456749288811424No Hit
GATTCAACCCGGCGGCGGGTCCGGCCGTGTCG865050.1598746595975472No Hit
GTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG860940.15911506783875187No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG842810.1557643625864502No Hit
AGTCGATGGACAACAGGTTAATATTCCTGTACTTGGTA818800.1513269421171859No Hit
ATCGCATGATGAGTAATAGAAAGGAGCTT816880.15097209633205522No Hit
GGCAGGATCAACCAGGTAGGTAGGC815480.15071335461373078No Hit
AGGATCAACCAGGTAGGTAGGG806140.1489871777214805No Hit
AGACTACGAGGTTGATAGGTTGGAAGTGTAAGTG766950.14174425776352678No Hit
GCGTTGGTGGTATAGTGGTGAGCATAGCT758990.1402731262793392No Hit
AGGATTCAACCCGGCGGCGGGTCCGGCCGTG742970.13731238175965513No Hit
TAGACTACGAGGTTGATAGGTTGGAAGTGTAAGT742060.1371441996427442No Hit
AGGCACAGCTGGGTAGCTAAGTACGGA714380.13202850624044365No Hit
GACTGCATAATTTGTGGTAGTGGGG712540.13168844569636007No Hit
AAGGCGAGCGAGAAAACCAATGTTAAGGAACTCT709770.13117650672510386No Hit
CGGAGTGGACGTACCGCTGGTGTTCCAGTTGT702750.12987910182321982No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCAAGG684760.12655427074275064No Hit
GGCACAGCTGGGTAGCTAAGTACGGAAA680670.12579837529421706No Hit
TAGACTACGAGGTTGATAGGTTGGAAGTGTA677750.1252587139959975No Hit
CGCTCAACGGATAAAAGCTACCCCGAGG673500.12447324806536969No Hit
GTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCAAGG665090.1229189496002921No Hit
AGGCGAGCGAGAAAACCAATGTTAAGGAA648180.1197937192739589No Hit
GAGTGGACGTACCGCTGGTGTTCCAGTTGTC612640.11322537593877963No Hit
TAAGGCGAGCGAGAAAACCAATGTTAAGGA578230.10686587413339083No Hit
GGTAGCTAAGTACGGAAAAGATAACCGCTG572550.10581612201904592No Hit
GTATGGGCCGGCGAGTTATGTTAATATGCAAGGTT547450.10117725264051469No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)