FastQCFastQC Report
Wed 30 Aug 2023
SRR11459636_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11459636_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81333991
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length18-45
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG1101375413.541391323093933No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTC23965222.946519616872114No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG20094442.4706078913550424No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTC19120382.3508473843365194No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG17556742.1585981192045525No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG14071441.730081092418052No Hit
AAGGCGAGCGAGAAAACCAATGTTAAGGAACT13156511.6175906085808576No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG12996361.597900194028349No Hit
AGGGGAGATACCATGATCACGAAGGTGGT12801291.5739163715696676No Hit
AAGGCGAGCGAGAAAACCAATGTTAAGG8384361.03085559886026No Hit
TGGCAGGGGAGATACCATGATCACGAAGGTGGT6990780.8595151810514253No Hit
AAGGCGAGCGAGAAAACCAATGTTAAGGAACTC6533560.8033000618400737No Hit
TTGGAAACAATAGCTAATACCGGATATAGTTATT5959720.7327465339798708No Hit
GGAGTGGACGTACCGCTGGTGTTCCAGTT5920260.727894933865965No Hit
ATCGCATGATGAGTAATAGAAAGGAGCTT5811900.7145720907756758No Hit
GGCTTGGTCTAGGGGTATGATTCTC5142240.6322375106368505No Hit
AAGGCGAGCGAGAAAACCAATGTTAAGGAAC4649430.5716466071362464No Hit
GGGAGATACCATGATCACGAAGGTGGT4648400.5715199688159899No Hit
GCGTTGGTGGTATAGTGGTGAGCATAGCT4350190.5348551013560862No Hit
AGGGCTTCTGTCGTGAGTGGCACACGTAGGG4208730.517462619042019No Hit
TAAGGCGAGCGAGAAAACCAATGTTAAGGAACT3948350.4854489434804693No Hit
GTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG3948070.4854145175293316No Hit
CGCTCAACGGATAAAAGCTACCCCGGGG3625260.44572508436233016No Hit
TAGACTACGAGGTTGATAGGTTGGAAGTGTAAGTG3587080.44103086002505404No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTC3452690.4245076329772137No Hit
AGGATTCAACCCGGCGGCGGGTCCGGCCGTGTCGG3441980.42319084034619675No Hit
TAGACTACGAGGTTGATAGGTTGGAAGTGTAAGT3154040.38778866759409364No Hit
AAGGCGAGCGAGAAAACCAATGTTAAGGA3050110.3750104922307329No Hit
GGAACAGTACTCAAGTGGCCGAAGAGGAC3022040.37155929062917864No Hit
AGACTACGAGGTTGATAGGTTGGAAGTGTAAGTG2918470.358825377202995No Hit
GGGAGCGTAGCTCAGATGGTTAGAGCACACG2891410.35549835492518744No Hit
GGAGTGGACGTACCGCTGGTGTTCCAGTTGT2807620.3451963890472312No Hit
TAAGGCGAGCGAGAAAACCAATGTTAAGGAACTC2729510.33559277817806826No Hit
TAGACTACGAGGTTGATAGGTTGGAAGTGTA2697140.33161289232689933No Hit
GGAACAGTACTCAAGTGGCCGAAGAGGACA2669730.32824283761016965No Hit
TGGCAGGGGAGATACCATGATCACGAAGGTGGTT2614630.3214683022255726No Hit
CGCTCAACGGATAAAAGCTACCCCGGGGA2544290.3128200115004808No Hit
AGGGCTTCTGTCGTGAGTGGCACACGTAGG2538570.31211673849866783No Hit
GTGATGCGAGACAGAGACTGAGTTTTAGAGC2427390.29844717690049166No Hit
ACGAATTGAATGATTTCATGTTTCCAAGAAAAGCTTC2425140.2981705397931352No Hit
AAGTAGTCAGAGCACGTTAAAGTGTGATG2410280.29634350538632737No Hit
TGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG2285270.28097354770159993No Hit
GGGCTTCTGTCGTGAGTGGCACACGTAGGG2225450.27361868914068166No Hit
AGGCGAGCGAGAAAACCAATGTTAAGGAACT2169910.2667900558328682No Hit
CAAGTAGTCAGAGCACGTTAAAGTGTGATG2166360.2663535839523724No Hit
GAGTGGACGTACCGCTGGTGTTCCAGTTGT2151060.26447245162234817No Hit
GTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG2126480.26145034491176017No Hit
AGACTACGAGGTTGATAGGTTGGAAGTGTAAGT2067950.2542540916257263No Hit
GGTAGCTAAGTACGGAAAAGATAACCGCTG2004980.24651194111450894No Hit
GGGGAGATACCATGATCACGAAGGTGGT2001150.24604104328287543No Hit
GGCTTGGTCTAGGGGTATGATTCTCG1894500.2329284443941771No Hit
AGAGATAGGAAGATAGGAAGTTGCTTTT1795050.22070108424901957No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCG1717660.21118599725421075No Hit
GATTCAACCCGGCGGCGGGTCCGGCCGTGTCG1703320.2094228967566586No Hit
AAGGCGAGCGAGAAAACCAATGTTAAGGAA1655790.2035790915510343No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCG1600240.19674922874496592No Hit
GTGATGCGAGACAGAGACTGAGTTTTAGAGCT1526900.1877320885433988No Hit
AGGCGAGCGAGAAAACCAATGTTAAGGAAC1526490.18768167911494715No Hit
AGCACATATACTAAAATTGGAACGATACAGAGAAGA1473980.1812255837783738No Hit
GGCTTTGGTCTAGGGGTATGATTCTC1470500.18079771838566241No Hit
ATCTTGAGTTGTGATGCATAGTGAAG1413850.17383261077155307No Hit
GCGTTGGTGGTATAGTGGTTAGCATAGCT1406230.17289573310130568No Hit
TGGCAGGGGAGATACCATGATCACGAAGGTGG1328520.1633413021623395No Hit
GGCAGGGGAGATACCATGATCACGAAGGTGGT1311050.1611933687109981No Hit
TAAGGCGAGCGAGAAAACCAATGTTAAGG1300730.1599245265119229No Hit
AGACTACGAGGTTGATAGGTTGGAAGTGTA1285670.1580729021400167No Hit
AGGCGAGCGAGAAAACCAATGTTAAGG1281450.1575540538764414No Hit
TAGACTACGAGGTTGATAGGTTGGAAGTGT1274170.15665897914686125No Hit
ACGAATTGAATGATTTCATGTTTCCAAGAAAAGCTT1261030.15504341843989924No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAG1149460.14132590640978138No Hit
GGGCTTCTGTCGTGAGTGGCACACGTAGG1139900.14015050607807997No Hit
GCGTTGTGGTATAGTGGTGAGCATAGCT1129830.13891240133537774No Hit
GCTGGTCCGAAGGTAGTGAGTTATCTCAA1121290.1378624098256779No Hit
AGCACATATACTAAAATTGGAACGATACAGAGAAG1113470.1369009421903322No Hit
AGACTACGAGGTTGATAGGTTGGAAGTGTAAGTGT1105760.13595299903579058No Hit
TGATGCGAGACAGAGACTGAGTTTTAGAGC1100050.13525095553223251No Hit
GCGTTGGTGGTATAGTGGTGAGCATAGCTG1041440.1280448662601593No Hit
AGAGATAGGAAGATAGGAAGTTGCTTT1017650.12511988991170986No Hit
CGGAGTGGACGTACCGCTGGTGTTCCAGTT1009930.12417071725891332No Hit
ATGTACACGGAGTCGACCCAACGCGTA1009160.12407604589328464No Hit
CGAATTGAATGATTTCATGTTTCCAAGAAAAGCTTC995060.12234245335385054No Hit
AAGGCGAGCGAGAAAACCAATGTTAAGGAACTCT982770.12083139999855656No Hit
GTTGGTCCGAGTGTTGTGGGTTATTGTT982670.1208191050160074No Hit
TTGGGAAAGGTTTCCTGGGCAAGGTTCGTCCACCCAGGG965930.118760924937275No Hit
GCGTTGGTGGTATAGTGGTAAGCATAGCT962950.11839453445730949No Hit
GCGTTGGTGGTATAGTGGTTAGCATAGCTG958290.11782158827051778No Hit
GGAACAGTACTCAAGTGGCCGAAGAGGA931560.11453513943512253No Hit
TAAGGCGAGCGAGAAAACCAATGTTAAGGAAC919920.1131040034663982No Hit
TAGACTACGAGGTTGATAGGTTGGAAGTGTAAGTGT911320.11204663496716888No Hit
TCGCTCAACGGATAAAAGCTACCCCGGGG866090.10648561436017569No Hit
TAGACTACGAGGTTGATAGGTTGGAAGTGTAAG857590.10544054084349555No Hit
AGTCGATGGACAACAGGTTAATATTCCTGTACTTGGTA855270.10515529724835462No Hit
AGACTACGAGGTTGATAGGTTGGAAGTGT852700.10483931619684075No Hit
AGGGGAGATACCATGATCACGAAGGTGG839400.10320408351780008No Hit
GAGTGGACGTACCGCTGGTGTTCCAGTT826990.10167827618344709No Hit
TCTTGAGTTGTGATGCATAGTGAAG823690.10127254175932422No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)