FastQCFastQC Report
Thu 24 Aug 2023
SRR11549049_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11549049_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86874356
Total Bases8.6 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGCCCTGGCGGAGCGCTGAGAAGACGGTCGAACTTGACTATCTAGAGG12000461.3813581536074926No Hit
CCGGCCCTGGCGGAGCGCTGAGAAGACGGTCGAACTTGACTATCTAGAGG7529170.8666734749665367No Hit
GCGCTGAGAAGACGGTCGAACTTGACTATCTAGAGGAAGTAAAAGTCGTA6186940.7121710346836989No Hit
GGGCCCTGGCGGAGCGCTGAGAAGACGGTCGAACTTGACTATCTAGAGGA5145140.592250721260023No Hit
GGCCCTGGCGGAGCGCTGAGAAGACGGTCGAACTTGACTATCTAGAGGAA5139480.5915992056390035No Hit
GAGCGCCGAAGGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGT4713920.5426135187695665No Hit
GATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCCGG3573010.4112847754520333No Hit
CGGCCCTGGCGGAGCGCTGAGAAGACGGTCGAACTTGACTATCTAGAGGA3449120.39702395031279425No Hit
GCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGC3445730.3966337315927844No Hit
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCCGG3385810.38973641427626815No Hit
GGGGCCGCCGGTGAAATACCACTACTCTCATCGTTTTTTCACTGACCCGG3365620.38741236827125375No Hit
GGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCA3191950.36742142871251904No Hit
CCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGAAAAAAA3126800.35992209254477814No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTAGAGGAAGTAAAAGTCGTAA3060320.3522696617169743No Hit
CCGCTGAGAAGACGGTCGAACTTGACTATCTAGAGGAAGTAAAAGTCGTA2806190.32301707076827135No Hit
CGCCCTGGCGGAGCGCTGAGAAGACGGTCGAACTTGACTATCTAGAGGAA2358670.27150359537629265No Hit
CCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGC2344780.26990473460315495No Hit
CCCCTGGCGGAGCGCTGAGAAGACGGTCGAACTTGACTATCTAGAGGAAG2340330.2693925005901627No Hit
GCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGAAAAAAA2306060.26544772314628723No Hit
GGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCG2232150.2569400341799368No Hit
CGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCA2230620.25676391776648105No Hit
CAGCGCCGAAGGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGT1755710.2020976132473431No Hit
GCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAAAA1712540.19712836777748313No Hit
GTACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGC1627260.1873118921307457No Hit
GCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGG1593760.18345574843743304No Hit
CCCCTGTAATTGGAATGAGTCCACTTTAAATCCTTTAACGAGGATCCATT1580500.181929406187483No Hit
CCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGG1531560.17629598313223754No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAAAAA1525270.1755719489880305No Hit
GCTACTTGGATAACTGTGGTAATTCTAGAGCTAAAAAAAAAAAAAAAGAT1479490.17030226963639306No Hit
GCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTCC1434130.16508093596688073No Hit
CCTACTTGGATAACTGTGGTAATTCTAGAGCTAAAAAAAAAAAAAAAGAT1424670.16399200703139602No Hit
GTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC1371140.15783023473578325No Hit
GGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCAC1301760.14984398848378228No Hit
CCCTGGCGGAGCGCTGAGAAGACGGTCGAACTTGACTATCTAGAGGAAGT1291440.14865606600870804No Hit
CTACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGC1282020.1475717414239019No Hit
CGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCG1250720.14396883701791124No Hit
CCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA1249860.1438698434783217No Hit
CTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC1231950.1418082454619865No Hit
GTACTTGGATAACTGTGGTAATTCTAGAGCTAAAAAAAAAAAAAAAGATC1182660.13613453433830347No Hit
GGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACC1148340.13218400145607986No Hit
CTACTTGGATAACTGTGGTAATTCTAGAGCTAAAAAAAAAAAAAAAGATC1112010.1280020999522575No Hit
GCACCTCACCCCGAGTGTTACAGCCCTCCGGCCGCAGCTTTCGCCGAATC1068180.12295688269620093No Hit
ACCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGAAAAAAA1066440.12275659344168262No Hit
GCGGGCCGCCGGTGAAATACCACTACTCTCATCGTTTTTTCACTGACCCG1058550.12184838527033225No Hit
CCCGTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCT1053820.12130392080258989No Hit
GCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA1051060.12098622060576772No Hit
GGGGGGGCGGGGCGGACTGTCCCCAGTGCGCCCCGGGCGTCGTCGCGCCG959630.11046182604219823No Hit
CCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTCC942120.10844627153264884No Hit
CTCCGTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTC941580.10838411279848797No Hit
GCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAG923750.10633172348350992No Hit
TCGGCCCTGGCGGAGCGCTGAGAAGACGGTCGAACTTGACTATCTAGAGG923340.10628452888905444No Hit
CCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGGAAAAAA902180.10384882738008441No Hit
GATCTAGAGGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCG896370.10318004544402032No Hit
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAAAAAAAAA883310.10167672494746322No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)