FastQCFastQC Report
Tue 18 Jul 2023
SRR11549060_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11549060_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55562300
Total Bases5.5 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCTGAGAAGACGGTCGAACTTGACTATCTAGAGGAAGTAAAAGTCGTA18561373.340641046176994No Hit
CCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCG10868471.9560871310222938No Hit
GCGGCCCTGGCGGAGCGCTGAGAAGACGGTCGAACTTGACTATCTAGAGG10721281.9295961470277507No Hit
GGCCCTGGCGGAGCGCTGAGAAGACGGTCGAACTTGACTATCTAGAGGAA10308881.8553731576986552No Hit
CTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC9816701.7667915115105026No Hit
GTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC9646861.73622402240368No Hit
CCGCTGAGAAGACGGTCGAACTTGACTATCTAGAGGAAGTAAAAGTCGTA8275091.4893353946830854No Hit
CCCCTGGCGGAGCGCTGAGAAGACGGTCGAACTTGACTATCTAGAGGAAG8159021.4684453307368486No Hit
CCGGCCCTGGCGGAGCGCTGAGAAGACGGTCGAACTTGACTATCTAGAGG7170521.2905369288168416No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTAGAGGAAGTAAAAGTCGTAA7029701.2651924056419552No Hit
GCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCG6994991.2589453640328063No Hit
GGGCCCTGGCGGAGCGCTGAGAAGACGGTCGAACTTGACTATCTAGAGGA6505341.1708190625658044No Hit
GGGGCCGCCGGTGAAATACCACTACTCTGATCGTTTTTTCACTGACCCGG6042801.0875719687629923No Hit
CGCCCTGGCGGAGCGCTGAGAAGACGGTCGAACTTGACTATCTAGAGGAA4752520.8553497605390705No Hit
CCCTGGCGGAGCGCTGAGAAGACGGTCGAACTTGACTATCTAGAGGAAGT4224190.7602619042048295No Hit
CCCCTGGCGGACGCTGAGAAGACGGTCGAACTTGACTATCTAGAGGAAGT4009060.7215432046549549No Hit
GGCCCTGGCGGACGCTGAGAAGACGGTCGAACTTGACTATCTAGAGGAAG3804470.6847214748129578No Hit
GGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCG3540810.6372684356119167No Hit
CGGCCCTGGCGGAGCGCTGAGAAGACGGTCGAACTTGACTATCTAGAGGA3191200.5743462743622924No Hit
GCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCA2811830.5060679633492494No Hit
CTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCCCAAAGATTAAGCC2528680.455107149991991No Hit
GTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCCCAAAGATTAAGCC2478980.4461622359045612No Hit
GATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCCGG2354410.4237423576777779No Hit
GCGGGCCGCCGGTGAAATACCACTACTCTGATCGTTTTTTCACTGACCCG2245210.40408874362652375No Hit
GCGGCCCTGGCGGACGCTGAGAAGACGGTCGAACTTGACTATCTAGAGGA2234200.40210718418783964No Hit
GGGCCCTGGCGGACGCTGAGAAGACGGTCGAACTTGACTATCTAGAGGAA2183240.39293549763058766No Hit
CCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCA1928680.3471202596004845No Hit
CCCTGGCGGACGCTGAGAAGACGGTCGAACTTGACTATCTAGAGGAAGTA1915990.34483633686870413No Hit
CCCCTGTAATTGGAATGAGTCCACTTTAAATCCTTTAACGAGGATCCATT1911090.34395444393050684No Hit
GCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTCCCGTGGATCG1792470.322605435700106No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCAC1785020.3212645984777448No Hit
CGCCCTGGCGGACGCTGAGAAGACGGTCGAACTTGACTATCTAGAGGAAG1758950.3165725680902338No Hit
GCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAG1735150.3122890881047041No Hit
GCCCTGGCGGAGCGCTGAGAAGACGGTCGAACTTGACTATCTAGAGGAAG1730130.3113855977884285No Hit
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCCGG1710340.3078238301870153No Hit
CGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCG1685640.3033783698658983No Hit
GGGCCGCCGGTGAAATACCACTACTCTGATCGTTTTTTCACTGACCCGGT1472440.265007028146783No Hit
CGGGCCGCCGGTGAAATACCACTACTCTGATCGTTTTTTCACTGACCCGG1445160.2600972241969825No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGC1418120.25523061500333855No Hit
GGACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC1383870.2490663633434901No Hit
GTACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGC1368160.24623890659673914No Hit
CGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTCCCGTGGATCGC1333670.24003146018073407No Hit
CGGCCCTGGCGGACGCTGAGAAGACGGTCGAACTTGACTATCTAGAGGAA1256590.22615874432843852No Hit
CCGGCCCTGGCGGACGCTGAGAAGACGGTCGAACTTGACTATCTAGAGGA1246790.22439495845204394No Hit
CCGGGCCGCCGGTGAAATACCACTACTCTGATCGTTTTTTCACTGACCCG1223460.22019606819732085No Hit
CCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCAAA1121230.20179690185611465No Hit
CTACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGC1117440.20111478466514165No Hit
GCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGG1114080.20051005807894923No Hit
CCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTCCCGTGGATCG1069350.1924596354002624No Hit
CCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAG1034680.18621979291713986No Hit
GATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGG1004470.18078265298592752No Hit
CCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGG984820.17724608232560565No Hit
GCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAAAA972420.17501435325751455No Hit
CCCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGG914770.1646386128723973No Hit
GGCTGAGAAGACGGTCGAACTTGACTATCTAGAGGAAGTAAAAGTCGTAA912020.1641436729581029No Hit
CGGGACCGGGGTCCGGTGCGGAGTGCCCTTCGTCCTGGGAAACGGGGCGC906370.1631267964069162No Hit
GCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTT905740.1630134101720051No Hit
GAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGC872950.1571119266121093No Hit
GCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAAT864080.1555155204158215No Hit
GGGGGGGGATGCGTGCATTTATCAGATCAAAACCAACCCGGTAAAAAAAA863770.15545972718911924No Hit
GCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCAAA857930.15440865478930857No Hit
CCTAAACCATTCGTAGACGACCTGCTTCTGGGTCGGGGTTTCGTACGTAG853390.15359155398534619No Hit
CGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGA830410.14945565608335148No Hit
GCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTC793380.142791065164689No Hit
GCGGGACCGGGGTCCGGTGCGGAGTGCCCTTCGTCCTGGGAAACGGGGCG776870.13981962589741603No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC769980.13857957643942023No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAAAAA753120.13554514481941893No Hit
CCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCAAAA736820.132611500963783No Hit
GTGGCGCTAAACCATTCGTAGACGACCTGCTTCTGGGTCGGGGTTTCGTA735300.13233793417479117No Hit
TCGCTGAGAAGACGGTCGAACTTGACTATCTAGAGGAAGTAAAAGTCGTA667720.1201750107536945No Hit
GTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGC651730.11729716012476087No Hit
GCTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA650730.11711718197410835No Hit
CCTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA649810.11695160207550803No Hit
GCGGCGGGGGACCGTCCCCCGACCGGCGACCGGCCGCCGCCGGGCGCATT649780.11694620273098846No Hit
GGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACTCTAGATAACCTCGGG628240.11306947336593338No Hit
CCCGTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCT625290.11253853782150847No Hit
GCTAAACCATTCGTAGACGACCTGCTTCTGGGTCGGGGTTTCGTACGTAG611980.11014302863632354No Hit
CCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTT610080.10980107015008378No Hit
GGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT600840.10813807203805459No Hit
GCCCTGGCGGACGCTGAGAAGACGGTCGAACTTGACTATCTAGAGGAAGT595360.10715179177247883No Hit
GATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAAAAAAAAA566350.10193062562204949No Hit
GTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAAA557530.10034321833329433No Hit

[FAIL]Adapter Content

Adapter graph