FastQCFastQC Report
Tue 18 Jul 2023
SRR11549062_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11549062_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98962432
Total Bases10.1 Gbp
Sequences flagged as poor quality0
Sequence length103
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTT2520180.25466027350661713TruSeq Adapter, Index 5 (98% over 50bp)
CCGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTT2367990.2392817104575603TruSeq Adapter, Index 5 (100% over 48bp)
TCGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTT2330400.23548329935949835TruSeq Adapter, Index 5 (100% over 48bp)
CTGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTT1998980.20199382327224943TruSeq Adapter, Index 5 (100% over 48bp)
TCTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC1894250.19141101948666744No Hit
ACTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC1719560.17375886639487598No Hit
CTTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC1586740.1603376117514978No Hit
TTTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC1572220.1588703883105864No Hit
TTGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTT1542470.15586419703185952TruSeq Adapter, Index 5 (100% over 48bp)
CCTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC1524620.1540604822646234No Hit
GCGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTT1451950.14671729166882236TruSeq Adapter, Index 5 (100% over 48bp)
GTTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC1308460.13221785010295625No Hit
ACGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATGCCGTCTT1175560.11878851158387052Illumina PCR Primer Index 5 (97% over 36bp)
TCGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATGCCGTCTT1167490.1179730506218764TruSeq Adapter, Index 5 (97% over 48bp)
AGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC1091710.11031559935794626No Hit
GCTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC1086260.10976488532537275No Hit
TTTCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAG1079850.10911716478430927No Hit
ACGGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCT1052550.1063585421991246TruSeq Adapter, Index 5 (97% over 48bp)
CCGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATGCCGTCTT1038470.10493578007460447TruSeq Adapter, Index 5 (97% over 48bp)
GATCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAG1005250.10157895068706477No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)