FastQCFastQC Report
Fri 25 Aug 2023
SRR11549063_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11549063_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94552160
Total Bases9.7 Gbp
Sequences flagged as poor quality0
Sequence length103
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTT9865751.0434187859907167TruSeq Adapter, Index 9 (98% over 50bp)
TCGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTT9505041.005269472426648TruSeq Adapter, Index 9 (100% over 48bp)
CCGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTT9371330.9911280715321574TruSeq Adapter, Index 9 (100% over 48bp)
CTGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTT7873730.8327393049508336TruSeq Adapter, Index 9 (100% over 48bp)
TTGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTT6777740.7168255066832953TruSeq Adapter, Index 9 (100% over 48bp)
GCGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTT6226390.6585137769459735TruSeq Adapter, Index 9 (100% over 48bp)
ACGGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCT5459880.5774463534201651TruSeq Adapter, Index 9 (97% over 48bp)
CCGGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCT5049050.5339962619574211TruSeq Adapter, Index 9 (97% over 48bp)
TCGGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCT4945370.5230308858094833TruSeq Adapter, Index 9 (97% over 48bp)
TCTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC4623500.4889893578316984No Hit
CTGGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCT4342730.4592946369495948TruSeq Adapter, Index 9 (97% over 48bp)
ACTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC4036160.42687126343808546No Hit
TGGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTT4025840.42577980238632307TruSeq Adapter, Index 9 (100% over 48bp)
AGGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTT3987160.4216889386768108TruSeq Adapter, Index 9 (98% over 50bp)
GTGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTT3946910.417432029051478TruSeq Adapter, Index 9 (100% over 48bp)
CCTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC3756350.39727807381661084No Hit
CTTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC3630880.38400814957585316No Hit
ATGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTT3604210.38118748424150223TruSeq Adapter, Index 9 (98% over 50bp)
TTTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC3602200.3809749031645602No Hit
GCGGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCT3571550.377733306145518TruSeq Adapter, Index 9 (100% over 47bp)
TTTCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAG3424220.36215143049085285No Hit
CAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTT3296790.3486742132596442TruSeq Adapter, Index 9 (98% over 50bp)
TTGGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCT3282530.34716605099238346TruSeq Adapter, Index 9 (97% over 48bp)
GTTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC3100760.32794174136265103No Hit
GATCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAG3026220.3200582620217243No Hit
CGGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTT2943870.31134878357088824TruSeq Adapter, Index 9 (100% over 48bp)
GTTCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAG2898400.3065397977158851No Hit
TAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTT2810190.2972105555282925TruSeq Adapter, Index 9 (98% over 50bp)
CTTCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAG2796920.2958070973735555No Hit
TCTCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAG2673310.2827338899502666No Hit
GCTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC2630450.2782009422100986No Hit
AGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC2619690.27706294599721465No Hit
AGTCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAG2218530.23463557046184877No Hit
GATCGGGGGGGATGCGTGCATTTATCAGATCAAAACCAACCCGGTCAGCC2178740.23042731123223414No Hit
TGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC2174340.22996195962101765No Hit
CCTCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAG2095720.2216469724224174No Hit
TTTCGGGGGGGATGCGTGCATTTATCAGATCAAAACCAACCCGGTCAGCC2066760.21858411272677433No Hit
TGTCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAG2055090.21734987333975236No Hit
ATTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC2048940.21669943870134747No Hit
TATCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAG2021840.21383329582317315No Hit
AGGGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCT2016160.21323256919778458TruSeq Adapter, Index 9 (97% over 48bp)
GTGGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCT1979740.20938072699766985TruSeq Adapter, Index 9 (100% over 47bp)
ACTCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAG1974000.208773654668492No Hit
GGGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTT1947920.20601538875473602TruSeq Adapter, Index 9 (100% over 48bp)
GTTCGGGGGGGATGCGTGCATTTATCAGATCAAAACCAACCCGGTCAGCC1931220.20424916786670977No Hit
GATACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC1928950.204009088740014No Hit
TGGGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCT1912530.2022724811363379TruSeq Adapter, Index 9 (97% over 48bp)
GCTCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAG1818680.19234674279254962No Hit
GAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTT1803450.19073599164736163TruSeq Adapter, Index 9 (98% over 50bp)
ATGGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCT1769120.18710519146257473TruSeq Adapter, Index 9 (97% over 48bp)
CATACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC1717960.18169442136488473No Hit
TATACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC1707510.18058921128824554No Hit
TCTCGGGGGGGATGCGTGCATTTATCAGATCAAAACCAACCCGGTCAGCC1702940.18010588018295934No Hit
CGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC1653520.1748791354951595No Hit
CTTCGGGGGGGATGCGTGCATTTATCAGATCAAAACCAACCCGGTCAGCC1614670.17077029229157747No Hit
CATCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAG1609200.1701917756294515No Hit
AAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTT1595030.16869313191787474TruSeq Adapter, Index 9 (100% over 50bp)
CGGGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCT1591300.16829864066563893TruSeq Adapter, Index 9 (97% over 48bp)
CGTCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAG1572380.166297628737408No Hit
ATTCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAG1535140.16235906191883928No Hit
ACTCGGGGGGGATGCGTGCATTTATCAGATCAAAACCAACCCGGTCAGCC1508410.15953205088069908No Hit
AGTCGGGGGGGATGCGTGCATTTATCAGATCAAAACCAACCCGGTCAGCC1493680.15797418060042204No Hit
CAGGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCT1491660.15776054190618172TruSeq Adapter, Index 9 (100% over 47bp)
GGTCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAG1381520.14611194498359426No Hit
GGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC1367940.1446757006926124No Hit
TATCGGGGGGGATGCGTGCATTTATCAGATCAAAACCAACCCGGTCAGCC1349230.14269689872764407No Hit
CCTCGGGGGGGATGCGTGCATTTATCAGATCAAAACCAACCCGGTCAGCC1333270.14100894151968607No Hit
TGTCGGGGGGGATGCGTGCATTTATCAGATCAAAACCAACCCGGTCAGCC1318730.13947116596807518No Hit
TTTCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACTCTAGATAACCTC1237140.13084206643190383No Hit
GCTCGGGGGGGATGCGTGCATTTATCAGATCAAAACCAACCCGGTCAGCC1137690.12032406240111279No Hit
TAGGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCT1119910.11844361884487886TruSeq Adapter, Index 9 (100% over 47bp)
GATCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACTCTAGATAACCTC1119010.11834843328803911No Hit
GTTCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACTCTAGATAACCTC1108700.117258029853575No Hit
TCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCG1092930.11559016737428315No Hit
CATCGGGGGGGATGCGTGCATTTATCAGATCAAAACCAACCCGGTCAGCC1067310.11288055185624528No Hit
AATCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAG1019700.10784523589942314No Hit
ATTCGGGGGGGATGCGTGCATTTATCAGATCAAAACCAACCCGGTCAGCC1017960.10766121048953296No Hit
ACTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCG1015850.1074380532396087No Hit
CTTCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACTCTAGATAACCTC1004830.10627255897697101No Hit
CGTCGGGGGGGATGCGTGCATTTATCAGATCAAAACCAACCCGGTCAGCC1003290.10610968591304525No Hit
GGGGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCT999920.10575326888354535TruSeq Adapter, Index 9 (100% over 47bp)
TCTCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACTCTAGATAACCTC997170.10546242412653503No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)