FastQCFastQC Report
Thu 24 Aug 2023
SRR11549064_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11549064_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76832728
Total Bases3.9 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAA16437432.139378677274091No Hit
GCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAA9854731.2826213849910417No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCAAAAAAAAAAAAAA9604451.2500467248800538No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAAA9174121.1940380406641293No Hit
GCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAAA8125991.0576209138376553No Hit
ACCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAA7589790.9878329453563071No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAGATC5513500.7175978445018899No Hit
GCGCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAGAT5333180.6941286791222616No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAAA5119950.6663761828162602No Hit
GGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTAAAAAAAA3977520.517685640421358No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCAAAAAAAAAAAAAAA3579460.4658769892954992No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAG2978730.3876902561627123No Hit
GGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGAAAAAAAAAAAAAAAGAT2956190.3847566104902588No Hit
GGCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAGATC2823870.36753478283369034No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAAAG2741700.3568401215690272No Hit
ACAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAAA2720850.35412643424557305No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAGATCG2680730.34890470113204886No Hit
GGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCAAAAAAAAA2471600.3216858315898923No Hit
CCGCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAGAT2397450.31203499633645704No Hit
GGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCAAAAAAAAAAAAAAAGATC2243510.291999263647127No Hit
GCGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTAAAAAAA2167150.2820607905526926No Hit
GGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCAAAAAAA2075780.2701687228911096No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTAAAAAAAA2002280.2606024870026742No Hit
CCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAGATCG1834540.23877064471796447No Hit
GCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCAAAAAAAAAAAAA1612980.20993397501127384No Hit
CCCCCGCCGCGCGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGA1476120.19212125332839933Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAGAT1357570.1766916306811337No Hit
TCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAA1316710.17137358444437897No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCAAAAAAAAA1268000.16503383818416548No Hit
GCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAGA1207900.15721165074341759No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAAG1204180.15672748206988044No Hit
GCGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCAAAAAA1203790.15667672245088055No Hit
GCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCAAAAAAAAAAAAAA1202850.15655437875380399No Hit
GCGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCAAAAAAAA1196910.15578127071057532No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCACAAAAAAAAAAAAAA1191770.155112284962731No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAGATCG1163580.1514432755791256No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAGAT1136050.1478601670892123No Hit
CGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAGATCGG1107670.14416642866045315No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGCAAAAAAAAAAAAAAAGATC1095190.14254212085245757No Hit
GCGCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAGATC1091470.14205795217892042No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCAAAAAAA1060590.1380388315770852No Hit
GCGCGCGCGGGTCGGGGGGCGGGGCGGACTGCAAAAAAAAAAAAAAAGAT1020850.13286655655386856No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAAA1017420.13242013221240823No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAGA991200.12900752398118678No Hit
CCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCAAAAAAAAAAAAA986850.12844135900003448No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGAAAAAAAAAAAAAAAGATCG977250.12719189145542248No Hit
GCGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCAAAAAAAAAAAAAAAGA933630.12151462329959183No Hit
CCGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTAAAAAAA928390.12083262226482444No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAAAAAAAAAAAAAGA868350.11301824399623035No Hit
GGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCCCAAAAAAA799520.10405982200710094No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAA780140.10153745940141551No Hit
GCGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCCAAAAAAAAAAAAAAAG775690.10095827913334016No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)