FastQCFastQC Report
Tue 25 Jul 2023
SRR11549065_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11549065_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59193462
Total Bases3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCAAAAAAAAAAAAAA8950461.5120690187034507No Hit
GCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAA7732611.3063283914699904No Hit
CCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAA7635811.2899752340891972No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAAA6707941.1332231252160923No Hit
GCGCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAGAT4529760.7652466753845214No Hit
GCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAAA4178240.7058617385818725No Hit
ACCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAA3899780.6588193810998925No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAGATC3318870.5606818536817462No Hit
GCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCAAAAAAAAAAAAA2881580.48680714096431793No Hit
GGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTAAAAAAAA2747880.4642201870199787No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCAAAAAAAAAAAAAAA2468280.4169852407010761No Hit
GGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGAAAAAAAAAAAAAAAGAT2460240.41562698258804326No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAG2191060.3701523658136434No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAAA2092090.35343261389239233No Hit
GGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCAAAAAAA2053610.3469318959583746No Hit
GGCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAGATC1964850.3319369966906142No Hit
CCGCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAGAT1943090.3282609150314607No Hit
GCGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTAAAAAAA1933530.3266458718025312No Hit
GGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCAAAAAAAAAAAAAAAGATC1868900.31572743624963173No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAGATCG1791930.30272431100583375No Hit
CCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCAAAAAAAAAAAAA1713920.2895454906827379No Hit
GCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCAAAAAAAAAAAAAA1689980.28550112510736403No Hit
GCGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCAAAAAA1572430.2656425130194277No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAAAG1525050.25763825065680396No Hit
GGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCAAAAAAAAA1499820.25337595560807036No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTAAAAAAAA1294200.2186390111799847No Hit
GCGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCAAAAAAAAAAAAAAAGA1284490.21699862731461797No Hit
GCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAGA1217700.20571528659702318No Hit
GCGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCCAAAAAAAAAAAAAAAG1168450.1973951109668159No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCACAAAAAAAAAAAAAA1140950.19274932761999966No Hit
ACAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAAA1093970.18481264028787503No Hit
CCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAGATCG1061300.17929344967185734No Hit
CCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCAAAAAAAAAAAAAA1044770.17650091153648015No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCAAAAAAA982520.16598454741505067No Hit
TCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAA979940.16554868846833118No Hit
GCGCGCGCGGGTCGGGGGGCGGGGCGGACTGCAAAAAAAAAAAAAAAGAT974710.16466514494455486No Hit
GCGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCAAAAAAAA939090.15864758847860597No Hit
GCGCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAGATC921070.15560333335461946No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAAG901480.15229384623592382No Hit
GGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCAAAAAA853470.14418315320026392No Hit
GGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCCCAAAAAAA805270.1360403620251169No Hit
CCCCCGCCGCGCGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGA802830.1356281543390721Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTAAAAAAA777020.13126787549611474No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAGAT748970.1265291764823622No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGCAAAAAAAAAAAAAAAGATC732200.12369609332868552No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCAAAAAAAAA717310.12118061281835484No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAGATCG703420.11883406988427202No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAGAT670930.11334528803197894No Hit
CCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCG652290.11019629161071877No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCAAAAAAAAAAAAAA642750.10858462713331414No Hit
GCGCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAAGA639920.1081065337925327No Hit
GCGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCCCAAAAAA639000.10795111122238465No Hit
GCGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCAAAAA627220.10596102657418484No Hit
CCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAGA624240.10545759259696619No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCAAAAAAAAAAAAAAAGAT623930.10540522194832935No Hit
CCGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCAAAAAA615790.10403007007767176No Hit
GCGCGCGCGGGTCGGGGGGCGGGGCGGACTGAAAAAAAAAAAAAAAGATC608900.10286609017732397No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)