FastQCFastQC Report
Tue 18 Jul 2023
SRR11549066_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11549066_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65414551
Total Bases3.3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAAA11632331.7782480842832662No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCAAAAAAAAAAAAAA9310381.42328883370307No Hit
CCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAA7901111.2078520572586366No Hit
GCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAA6939581.0608618256815674No Hit
GCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAAA4623480.7067968715400951No Hit
GCGCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAGAT4087380.6248426286683524No Hit
ACCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAA3963280.6058713144725246No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAGATC3862780.5905077602688124No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAG3686780.5636024315140525No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCAAAAAAAAAAAAAAA2932050.44822596122382613No Hit
GCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCAAAAAAAAAAAAA2931950.4482106741052155No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAAAG2919520.44631048526191064No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAAA2431350.37168335834025673No Hit
GGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGAAAAAAAAAAAAAAAGAT2201030.336474066756187No Hit
GCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCAAAAAAAAAAAAAA2161610.3304478845998653No Hit
GGCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAGATC1945630.297430765824564No Hit
CCGCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAGAT1804930.27592178993936683No Hit
GGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTAAAAAAAA1712340.26176744681775777No Hit
CCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCAAAAAAAAAAAAA1642930.2511566577900993No Hit
GGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCAAAAAAAAAAAAAAAGATC1583500.2420715231997847No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAAG1564410.23915321225700992No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAGATCG1428600.21839177647187397No Hit
GCGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCAAAAAAAAAAAAAAAGA1395020.2132583620424147No Hit
GCGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCCAAAAAAAAAAAAAAAG1388370.21224176865480585No Hit
CCCCCGCCGCGCGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGA1365260.20870891554388257Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAGA1362320.2082594742567292No Hit
ACAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAAA1284220.19632023462180453No Hit
CCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCAAAAAAAAAAAAAA1270880.19428093299914265No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAGAT1110650.1697863828492838No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAGA1094710.16734961614274477No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCACAAAAAAAAAAAAAA1079910.16508712258836725No Hit
GCGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTAAAAAAA1066830.16308756747409303No Hit
GGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCAAAAAAA1045400.1598115379558288No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAAAAAAAAAAAAAGA1018100.15563815457511893No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAA1017000.15546999627040167No Hit
TCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAA988580.1511253971612524No Hit
GAGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAA968530.14806032987981527No Hit
CCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAGATCG942070.1440153582954349No Hit
GGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCAAAAAAAAA930190.14219924860448863No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAGAT907130.13867403905287065No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTAAAAAAAA901700.13784394851231188No Hit
GCGCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAGATC883720.13509532458611542No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAGATCG856630.1309540441544879No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAAAAAAAAAAAAAGA830640.12698092202757763No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCAAAAAAAAAAAAAAAGAT825490.1261936354191287No Hit
CCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAGA759930.11617140045798068No Hit
GCGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCAAAAAA711700.10879842315205984No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCCAAAAAAAAAAAAAAAGA710780.10865778166084178No Hit
GCGCGCGCGGGTCGGGGGGCGGGGCGGACTGCAAAAAAAAAAAAAAAGAT686840.10499804546545002No Hit
CCGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCCAAAAAAAAAAAAAAAG655890.10026668225545107No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)