FastQCFastQC Report
Tue 18 Jul 2023
SRR11549067_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11549067_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63534458
Total Bases3.2 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAAA8955171.4094981340676582No Hit
GCGCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAGAT7690931.2105131989951028No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCAAAAAAAAAAAAAA7433521.1699981764226273No Hit
GGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCAAAAAAAAAAAAAAAGATC5793560.9118768275319198No Hit
CCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAA5698070.8968471880251186No Hit
GCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAA5296950.8337129436124252No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAGATC5010930.7886948527994052No Hit
GGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGAAAAAAAAAAAAAAAGAT4619900.7271487229811577No Hit
ACCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAA3638360.5726593276360364No Hit
GCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAAA3512640.55287164014211No Hit
CCGCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAGAT3102240.4882767710082614No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAG3041750.4787559531868517No Hit
GGCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAGATC2759610.43434855460638383No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCAAAAAAAAAAAAAAA2312440.3639662748047682No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAGATCG2300550.36209484938078795No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAAAG2230640.35109137155148157No Hit
GCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCAAAAAAAAAAAAA2187950.3443721830443568No Hit
GCGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCAAAAAAAAAAAAAAAGA1810940.2850327297983718No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAAA1722200.2710655059023247No Hit
GCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCAAAAAAAAAAAAAA1658080.26097334457468735No Hit
GCGCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAGATC1534140.2414658200121893No Hit
GCGCGCGCGGGTCGGGGGGCGGGGCGGACTGCAAAAAAAAAAAAAAAGAT1528490.2405765387972618No Hit
GCGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCCAAAAAAAAAAAAAAAG1507850.23732790795193376No Hit
CCCCCGCCGCGCGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGA1362690.21448046349903543Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCAAAAAAAAAAAAA1322960.20822716391158952No Hit
CCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAGATCG1307650.20581744791149395No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAGAT1285460.20232485496295569No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAAG1182410.18610530997210994No Hit
ACAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAAA1170030.1841567610445343No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAGAT1139370.17933103324813127No Hit
GCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAGA1080470.17006047332614374No Hit
GCGCGCGCGGGTCGGGGGGCGGGGCGGACTGAAAAAAAAAAAAAAAGATC1077790.16963865497994804No Hit
GCGCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAAGA1066740.16789944127641726No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAGATCG1055340.1661051393560326No Hit
CCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCAAAAAAAAAAAAAA1050670.1653701051482961No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGCAAAAAAAAAAAAAAAGATC1004520.15810633026884402No Hit
GGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTAAAAAAAA948850.1493441558909655No Hit
GGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCAAAAAAAAAAAAAAGATCG940110.14796852441867056No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCACAAAAAAAAAAAAAA909010.14307354286393692No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGAAAAAAAAAAAAAAAGATCG903660.1422314801205985No Hit
GGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCAAAAAAAAAAAAAAAAGAT882620.13891989131315166No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAGA867780.13658415091854564No Hit
GGGGGGTCTCCCCCGCGGGGGCGCGCCGGCAAAAAAAAAAAAAAAGATCG867340.136514897160215No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCAAAAAAAAAAAAAAAGATCGGAA858220.13507945562390725No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCAAAAAAAAAAAAAAAGAT857290.13493307836198115No Hit
ACGCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAGAT856040.13473633473036004No Hit
TCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAA796710.12539809499909482No Hit
GGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGAAAAAAAAAAAAAAAAGA795170.1251557068449376No Hit
CGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAGATCGG766910.12070772682124713No Hit
CCGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCAAAAAAAAAAAAAAAGA757520.11922978866050922No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAA740910.11661545928352769No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAAAAAAAAAAAAAGA737720.11611336953563058No Hit
GGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGAAAAAAAAAAAAAAAGATC728110.11460080449572733No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCCAAAAAAAAAAAAAAAGA681860.10732129012574562No Hit
CCGCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAGATC678320.10676411216099459No Hit
GGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGAAAAAAAAAAAAAAGATC663050.1043606919571109No Hit
CCGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCCAAAAAAAAAAAAAAAG652110.1026387916931628No Hit
TGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAAA644010.10136389296025788No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAAAAAAAAAAAAAGA642150.10107113843640565No Hit
CCGCGCGCGGGTCGGGGGGCGGGGCGGACTGCAAAAAAAAAAAAAAAGAT638310.10046674200006554No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)