FastQCFastQC Report
Fri 14 Jul 2023
SRR11626659_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11626659_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60962537
Total Bases3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG59012009.68004333546683TruSeq Adapter, Index 5 (100% over 49bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGT20105143.297950018057812No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG15751132.5837392561270867No Hit
AGATCNGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG12188351.9993180401924544TruSeq Adapter, Index 5 (97% over 49bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG11910441.953731026646742Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA10373321.7015892891727915Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA9123021.4964961185916523Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT7236841.18709626536704Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACG6594701.0817627225717328No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA6408581.051232497098997Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA5701290.9352120631068881Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG5464590.8963849388354688Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT5387430.8837279852706917No Hit
GTGGGNGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGT4214190.6912753647375272No Hit
GTGGGNGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG3298670.5410978877076589No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGTC3277380.5376055789804155No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC3230450.5299074085450216No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG3171110.5201735616744428Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACGT3073860.5042211415840518No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT2528350.4147383170749603Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGACNGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG2498580.40985499012286847Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGACTNCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA2187140.3587678773932916Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC2177330.3571586923949704Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGACNGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA1924060.31561350538938365Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGA1841160.30201498996014553Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC1544840.2534080889711004No Hit
CTCGANTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT1517230.2488790779819416Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC1411330.231507753688138TruSeq Adapter, Index 5 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC1405720.23058751639552008TruSeq Adapter, Index 5 (100% over 50bp)
AGTCCNCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACG1388180.22771033954836886No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC1386380.22741507624592458No Hit
CGACTNCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA1336720.21926908980182372Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC1263310.20722726811713887No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACAC1236130.20276879225023065No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC1192020.1955332009886662No Hit
TCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCG1187330.1947638760506309TruSeq Adapter, Index 5 (100% over 50bp)
GACTGNATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA1177460.19314484894222822Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCGANTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG1143720.18761030237307874Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGCCNAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT1131560.18561563472989978No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACACGTCTG1064730.17465316445081672Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAAC1043140.17111164517316593Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCA1009110.16552952840528928No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT810580.13296362649736837No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCT744090.1220569281754137Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACG728280.11946353216894501No Hit
TGGGGNGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGTC684610.11230011638131136No Hit
GTTGANCCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC676820.11102228242239984No Hit
CGACTNCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG670560.10999542227056594Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGGNGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACGT641880.10529089365162084No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACGT638450.10472825302529651No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT637930.10464295473792372No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTC611980.10038624212768572No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)