FastQCFastQC Report
Mon 17 Jul 2023
SRR11626660_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11626660_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62020417
Total Bases3.1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG37629356.067252014767976TruSeq Adapter, Index 7 (100% over 49bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC29742594.795612709279268No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC13871032.236526400652869No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC11859321.912163860491296No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT11812721.904650205754018No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA11037741.7796945802541122Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC9046651.4586567516951716No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT8479551.3672191207614743No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCT8238971.3284286689010816Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCNGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG7751701.2498626057286908TruSeq Adapter, Index 7 (97% over 49bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACG6911301.114358840895894No Hit
CACTCNCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC6164380.993927531960967No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT5644230.9100599887936903No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG5143850.8293801055868424No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC4224180.6810950658393671No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACAC4166130.6717352448629941No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA4117610.6639120146515622Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGATCGCAGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCACACGT3250040.5240274343850349No Hit
GTTGANCCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC2898460.46733965042511727No Hit
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC2617170.421985231089304Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACACG2614900.4216192225860074No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT2607040.4203518979886897Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA2545900.4104938539836003Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCGNGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC2473080.39875255917740765No Hit
CACTCNCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT2458180.39635012450819224No Hit
CGACTNCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA2297350.3704183414310162Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGA2290920.3693815860670527Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCT2272060.36634065198239474Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCGCNGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC1886780.3042191735021711No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG1811800.2921296062875553Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGCGNCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT1765930.28473365472534634No Hit
CACTCNCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCT1717090.27685882860155553Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT1713370.2762590261203823Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA1441700.23245570889986114Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTCCNCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACG1437210.23173175375457408No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG1333300.21497759358825339Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTC1314670.21197374406560343No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACG1278120.20608052345085004No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTG1270920.2049196154227728Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAAGATCGGAAGAGCACACGT1243840.2005533113393933No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG1213710.19569523371634215No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA1183500.19082425711520126No Hit
CCGCCNAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT1177450.18984877189716412No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC1172280.1890151754381142No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGAAGATCGGAAGAGCACACGTC1096630.17681757928199676No Hit
GGTGCNCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG1072370.17290596417628085No Hit
GAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAA1031680.16634522144538305Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC1027950.1657438065919486TruSeq Adapter, Index 7 (100% over 50bp)
GAATACAAGCTTGGGCTGCAGGTCGACAGATCGGAAGAGCACACGTCTGA1004930.16203212564662375Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCT1004860.16202083904079523Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA996800.16072126699825318Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC912980.1472063627047203TruSeq Adapter, Index 7 (100% over 50bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT894960.14430086789000468Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC892660.14393002226992443No Hit
CCGCCNAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC884440.1426046522712029No Hit
CGGTGNGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACAC866860.13977010183598088No Hit
TCGACNGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA863390.13921060866133808Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAA837210.13498941808146825Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACGT831120.1340074833743862No Hit
TCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCG818580.1319855685588183TruSeq Adapter, Index 7 (100% over 50bp)
ATAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGT768570.12392209488046493No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACAC742490.11971702802320726No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCT686700.11072160317786962Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCNCAGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCACACGT673520.10859649653758374No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC634020.10222762610577095No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)